Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
475,667 |
T→C |
S97P (TCA→CCA) |
ybaY → |
outer membrane lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 475,667 | 0 | T | C | 100.0%
| 90.7
/ NA
| 26 | S97P (TCA→CCA) | ybaY | outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/13); total (13/13) |
TCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCA > NC_000913/475539‑475800
|
tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAg < 1:249795/139‑1 (MQ=255)
tGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc < 1:154081/139‑1 (MQ=255)
tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGaa < 1:303702/139‑1 (MQ=255)
tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGaa < 2:652151/139‑1 (MQ=255)
gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCtt > 2:83787/1‑93 (MQ=255)
gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCtt < 2:312692/93‑1 (MQ=255)
gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCtt < 1:83787/93‑1 (MQ=255)
gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCtt > 1:312692/1‑93 (MQ=255)
gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTAtt > 2:79162/1‑139 (MQ=255)
gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTAtt > 1:656076/1‑139 (MQ=255)
aCGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc > 2:565229/1‑139 (MQ=255)
tcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGa > 1:506655/1‑139 (MQ=255)
cGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaa > 1:510002/1‑139 (MQ=255)
gTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaaa < 1:79162/139‑1 (MQ=255)
tCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAAc < 2:56328/139‑1 (MQ=255)
aaGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGt < 2:510002/139‑1 (MQ=255)
aaaGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATa < 1:565229/139‑1 (MQ=255)
tGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTAttt < 1:691504/122‑1 (MQ=255)
tGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTAttt > 2:691504/1‑122 (MQ=255)
tGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGTGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGcc < 2:546836/135‑1 (MQ=255)
tGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGcc > 1:546836/1‑135 (MQ=255)
aGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATc < 1:468782/139‑1 (MQ=255)
ggTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCa > 1:550583/1‑139 (MQ=255)
aaaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAAcc > 1:651850/1‑139 (MQ=255)
aaaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTACAGCCGGTGATCAAcc > 2:533879/1‑139 (MQ=255)
aaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCTATACCGTTCCGCCGGTGATCAACCa > 1:735366/1‑139 (MQ=255)
|
TCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCA > NC_000913/475539‑475800
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
