Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	913,349	G→A	L156L (CTG→TTG)	hcp ←	hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	913,349	0	G	A	100.0%	106.5 / NA	34	L156L (CTG→TTG)	hcp	hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/14); total (20/14)

TCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCA  >  NC_000913/913223‑913467

tCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACg                                                                                                            <  2:71792/139‑1 (MQ=255)

  atatCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCa                                                                                                          <  2:536643/139‑1 (MQ=255)

                      cAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCAcag                                                                                                                  >  2:758477/1‑111 (MQ=255)

                      cAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCAcag                                                                                                                  <  1:758477/111‑1 (MQ=255)

                           aCGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                  <  1:109483/138‑1 (MQ=255)

                           aCGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                  >  2:109483/1‑138 (MQ=255)

                                              cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGcac                                                                                                                    <  2:65878/85‑1 (MQ=255)

                                              cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGcac                                                                                                                    >  1:65878/1‑85 (MQ=255)

                                              cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCg                                                                                                     <  1:51871/100‑1 (MQ=255)

                                              cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCg                                                                                                     >  2:51871/1‑100 (MQ=255)

                                              cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                              >  2:72307/1‑139 (MQ=255)

                                              cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                              >  2:584871/1‑139 (MQ=255)

                                              cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                              >  1:644511/1‑139 (MQ=255)

                                               tGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                              >  2:95782/1‑138 (MQ=255)

                                               tGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                              <  1:95782/138‑1 (MQ=255)

                                                 ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTa                                                           >  2:610254/1‑139 (MQ=255)

                                                           tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctgctg                                                 >  2:174874/1‑139 (MQ=255)

                                                                   gTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGc                                                                                                       >  1:657746/1‑77 (MQ=255)

                                                                   gTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGc                                                                                                       <  2:657746/77‑1 (MQ=255)

                                                                        gACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAg                                    <  1:72307/139‑1 (MQ=255)

                                                                           cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTc                                 >  1:220513/1‑139 (MQ=255)

                                                                              gaACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGcc                              <  1:584871/139‑1 (MQ=255)

                                                                                    gcgcGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGtt                                                                  >  2:271714/1‑97 (MQ=255)

                                                                                    gcgcGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGtt                                                                  <  1:271714/97‑1 (MQ=255)

                                                                                     cgcgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtcg                       >  2:74270/1‑139 (MQ=255)

                                                                                                     gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCa       >  2:604697/1‑139 (MQ=255)

                                                                                                      ccgccgCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGcc                                                                                                      <  2:443417/43‑1 (MQ=255)

                                                                                                      ccgccgCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGcc                                                                                                      >  1:443417/1‑43 (MQ=255)

                                                                                                      ccgccgCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTc                 <  1:476851/128‑1 (MQ=255)

                                                                                                      ccgccgCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTc                 >  2:476851/1‑128 (MQ=255)

                                                                                                       cgccgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAgg     >  1:576725/1‑139 (MQ=255)

                                                                                                          cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                             <  1:438224/64‑1 (MQ=255)

                                                                                                          cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                             >  2:438224/1‑64 (MQ=255)

                                                                                                          cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCa  >  1:101983/1‑139 (MQ=255)

TCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCA  >  NC_000913/913223‑913467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A