Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,068,254	G→A	58 (TAG→TAA)	ymdF →	KGG family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,068,254	0	G	A	100.0%	132.1 / NA	42	58 (TAG→TAA)	ymdF	KGG family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/25); total (17/25)

ACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAA  >  NC_000913/1068121‑1068387

aCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                  <  1:748965/139‑1 (MQ=255)

aCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                  <  1:676760/139‑1 (MQ=255)

                        gTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTg                                                                                                         >  1:14960/1‑139 (MQ=255)

                                  tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGTAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCg                                                                                              >  1:598378/1‑139 (MQ=255)

                                  tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCg                                                                                              >  1:248897/1‑139 (MQ=255)

                                              cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCAt                                                                                  >  1:309828/1‑139 (MQ=255)

                                              cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCAt                                                                                  >  1:601259/1‑139 (MQ=255)

                                               gggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATc                                                                                 >  2:708901/1‑139 (MQ=255)

                                                  ggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCAcc                                                                              <  1:290913/139‑1 (MQ=255)

                                                   gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAtc                                                                                                                 <  1:231072/105‑2 (MQ=255)

                                                   gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAtc                                                                                                                 >  2:231072/1‑104 (MQ=255)

                                                       tttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCgg                                                                         <  1:658325/139‑1 (MQ=255)

                                                            aGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                    <  2:112915/139‑1 (MQ=255)

                                                              aaTGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                  <  2:77418/139‑1 (MQ=255)

                                                              aaTGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                  <  2:232968/139‑1 (MQ=255)

                                                               aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                  <  1:743490/139‑1 (MQ=255)

                                                                  aCCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtgg                                                              >  1:81106/1‑139 (MQ=255)

                                                                   cccGCAGCGCGCATCTGAAGCTGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCCGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtggt                                                             <  2:448834/139‑1 (MQ=255)

                                                                                    aaGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                            >  2:769127/1‑139 (MQ=255)

                                                                                    aaGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                            <  2:14960/139‑1 (MQ=255)

                                                                                         ggT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CAttt                                                                                                            <  1:158947/71‑1 (MQ=255)

                                                                                         ggT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CAttt                                                                                                            >  2:158947/1‑71 (MQ=255)

                                                                                         ggT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                       <  2:24959/139‑1 (MQ=255)

                                                                                         ggT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                       <  2:403784/139‑1 (MQ=255)

                                                                                           t‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCt                                     >  2:629492/1‑139 (MQ=255)

                                                                                                     tggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                            >  1:135848/1‑123 (MQ=255)

                                                                                                     tggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                            <  2:135848/123‑1 (MQ=255)

                                                                                                     tggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATg                            >  2:374048/1‑139 (MQ=255)

                                                                                                      ggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATa                                                        <  1:299816/110‑1 (MQ=255)

                                                                                                      ggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATa                                                        >  2:299816/1‑110 (MQ=255)

                                                                                                      ggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                            <  1:630863/122‑1 (MQ=255)

                                                                                                      ggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                            >  2:630863/1‑122 (MQ=255)

                                                                                                       gtAAGGGCAGTCACGGCAAAAGCAACAACTAACCTGTATAATCAATAACGAAT‑CATTTTTG‑ATGTAGCTAGTCAAAAGGCCATCACCGCGGGTTAGCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                          <  2:309828/139‑1 (MQ=255)

                                                                                                         aaGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGcgcg                        <  1:769127/139‑1 (MQ=255)

                                                                                                         aaGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGcgcg                        <  1:708901/139‑1 (MQ=255)

                                                                                                         aaGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGcgcg                        <  2:601259/139‑1 (MQ=255)

                                                                                                               aGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAtcatt                                                                                                              <  1:475344/49‑5 (MQ=255)

                                                                                                               aGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACAAAtcatt                                                                                                              >  2:475344/1‑45 (MQ=255)

                                                                                                                 tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTg                                                           >  1:358800/1‑96 (MQ=255)

                                                                                                                 tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTg                                                           <  2:358800/96‑1 (MQ=255)

                                                                                                                  cACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATc               <  1:307104/139‑1 (MQ=255)

                                                                                                                               aCAACTAACCGGGCTAATCAATGACGAAT‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa  <  2:598378/139‑1 (MQ=255)

ACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAA  >  NC_000913/1068121‑1068387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A