Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I2 R1 762 35.5 1545288 95.5% 1475750 117.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,210,944 G→A pseudogene (1845/2001 nt) yehQ → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,210,9440GA100.0% 78.0 / NA 25pseudogene (1845/2001 nt)yehQpseudogene
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

GCGTTTTGCCTGTGCCGTCTACTGCTCATCAGTTGCTGATACGCTGCCAGGCGTTACTTATTCAACTGCTCCATAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTC  >  NC_000913/2210826‑2211076
                                                                                                                      |                                                                                                                                    
gCGTTTTGCCTGTGCCGTCTACTGCTCATCAGTTGCTGATACGCTGCCAGGCGTTACTTATTCAACTGCTCCATAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCAc                                                                                                                  >  2:299767/1‑139 (MQ=255)
                          catcaGTTGCTGATACGCTGCCAGGCGTTACTTATTCAACTGCTCCATAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGcac                                                                                        >  1:9782/1‑139 (MQ=255)
                           atcaGTTGCTGATACGCTGCCAGGCGTTACTTATTCAACTGCTCCATAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGcaca                                                                                       <  1:494991/139‑1 (MQ=255)
                                        aCGCTGCCAGGCGTTACTTATTCAACTGCTCCATAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACaa                                                                          <  2:24524/139‑1 (MQ=255)
                                                 ggCGTTACTTATTCAACTGCTCCATAACGGCTGGCGCTATCAGGAAC‑GAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGcaca                                                                                       >  1:707772/1‑116 (MQ=255)
                                                 ggCGTTACTTATTCAACTGCTCCATAACGGCTGGCGCTATCAGGAAC‑GAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGcaca                                                                                       <  2:707772/116‑1 (MQ=255)
                                                             tCAACTGCTCCATAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAg                                                     >  2:615522/1‑139 (MQ=255)
                                                              cAACTGCTCCATAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAgg                                                    <  1:456261/139‑1 (MQ=255)
                                                                  tGCTCCATAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACg                                                >  1:573941/1‑139 (MQ=255)
                                                                         tAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGaa                                         >  1:213645/1‑139 (MQ=255)
                                                                            cGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCa                                      <  1:299767/139‑1 (MQ=255)
                                                                                      tATCAGGAACAGAGTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACg                            <  2:246199/139‑1 (MQ=255)
                                                                                                  agTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCa                                                                                                                   >  1:155941/1‑40 (MQ=255)
                                                                                                  agTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCa                                                                                                                   <  2:155941/40‑1 (MQ=255)
                                                                                                  agTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTAt                                                                                                  <  1:30813/57‑1 (MQ=255)
                                                                                                  agTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTAt                                                                                                  >  2:30813/1‑57 (MQ=255)
                                                                                                  agTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTtttgcttt                >  1:523604/1‑139 (MQ=255)
                                                                                                  agTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTtttgcttt                >  2:403630/1‑139 (MQ=255)
                                                                                                  agTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAACACAGAAAg                                                         >  2:184372/1‑98 (MQ=255)
                                                                                                  agTGCTATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAACACAGAAAg                                                         <  1:184372/98‑1 (MQ=255)
                                                                                                       tATTGGTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGaa           >  1:465965/1‑139 (MQ=255)
                                                                                                            gTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACaa                                                                          <  1:601063/71‑1 (MQ=255)
                                                                                                            gTCAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACaa                                                                          >  2:601063/1‑71 (MQ=255)
                                                                                                              cAGGCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAattat    >  2:557034/1‑139 (MQ=255)
                                                                                                                ggCATAATTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTc  >  1:34765/1‑139 (MQ=255)
                                                                                                                      |                                                                                                                                    
GCGTTTTGCCTGTGCCGTCTACTGCTCATCAGTTGCTGATACGCTGCCAGGCGTTACTTATTCAACTGCTCCATAACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTC  >  NC_000913/2210826‑2211076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A