Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,220,789 |
G→A |
R401C (CGT→TGT) |
bglX ← |
beta‑D‑glucoside glucohydrolase, periplasmic |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,220,789 | 0 | G | A | 100.0%
| 81.3
/ NA
| 26 | R401C (CGT→TGT) | bglX | beta‑D‑glucoside glucohydrolase, periplasmic |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (9/17); total (9/17) |
CCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACC > NC_000913/2220655‑2220920
|
cccGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGttt > 2:28166/1‑139 (MQ=255)
ggTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTc > 1:754745/1‑139 (MQ=255)
gTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCa > 1:768320/1‑139 (MQ=255)
tCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAg > 1:329146/1‑139 (MQ=255)
aCGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAaca < 1:511171/139‑1 (MQ=255)
aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTcgcgc > 2:599307/1‑139 (MQ=255)
aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTcgcgc < 2:161831/139‑1 (MQ=255)
aaCACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACt > 1:29660/1‑139 (MQ=255)
ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAc > 2:103711/1‑139 (MQ=255)
cgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCgg < 2:157624/139‑1 (MQ=255)
tttACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa < 1:28166/139‑1 (MQ=255)
tttACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTCTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa < 2:411131/139‑1 (MQ=255)
ccGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATc > 2:365394/1‑139 (MQ=255)
ggCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGt < 1:84923/139‑1 (MQ=255)
gCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTa < 1:14513/128‑1 (MQ=255)
gCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTa > 2:14513/1‑128 (MQ=255)
ccGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCttt < 1:405149/139‑1 (MQ=255)
aTGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGcc < 2:754745/139‑1 (MQ=255)
gCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAAt < 1:365394/139‑1 (MQ=255)
ccGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATg < 2:768320/139‑1 (MQ=255)
ttCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATg < 1:599307/139‑1 (MQ=255)
ttCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATg < 2:212759/139‑1 (MQ=255)
ttCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATg < 1:585870/139‑1 (MQ=255)
tttCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAaacaa < 2:371652/139‑1 (MQ=255)
tCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAAcc < 1:103711/139‑1 (MQ=255)
tCGAGACAGTTTGTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCTAATGGCTGTATGGGTCGTTAAACAAcc < 2:460670/139‑1 (MQ=255)
|
CCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACC > NC_000913/2220655‑2220920
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
