Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,629,481	G→A	64 (TAG→TAA)	yfgG →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,629,481	0	G	A	100.0%	100.5 / NA	32	64 (TAG→TAA)	yfgG	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/21); total (11/21)

ACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATC  >  NC_000913/2629352‑2629612

aCTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCt                                                                                                                            >  1:629256/1‑139 (MQ=255)

 cTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCtt                                                                                                                           >  1:558526/1‑139 (MQ=255)

       atttccTTTATCTTCTTCTTTGGCCGTTTGATCTACTCGTCCGTCGGTGCCAGGCAGCACCATCAGAGCAAAAAAGAAGATCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACATTAACGGTTACCTTCTCCAc                                                                                                                     <  2:488866/134‑1 (MQ=255)

         caGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACtt                                                                                                                   >  2:110282/1‑139 (MQ=255)

              ttATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCGCACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTcaca                                                                                                              >  1:409573/1‑139 (MQ=255)

                 tcttcttctTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGaa                                                                                                           <  1:250710/139‑1 (MQ=255)

                                             gtccgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCtt                                                                               >  1:590738/1‑139 (MQ=255)

                                                cgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTg                                                                            >  2:85504/1‑139 (MQ=255)

                                                cgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTg                                                                            <  1:110282/139‑1 (MQ=255)

                                                cgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTg                                                                            <  2:529862/139‑1 (MQ=255)

                                                    ggTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGt                                                                        <  2:558526/139‑1 (MQ=255)

                                                         cTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTggg                                                                   <  2:759759/139‑1 (MQ=255)

                                                              agcaCCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTAt                                                              <  2:697024/139‑1 (MQ=255)

                                                                 aCCATAAGCGCAAAAAAGAAGCTCAGCAATCCATGCTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCCCAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCaa                                                           <  2:760420/139‑1 (MQ=255)

                                                                  ccATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCaaa                                                          <  1:726429/139‑1 (MQ=255)

                                                                       agagCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGc                                                     <  2:567941/139‑1 (MQ=255)

                                                                           cAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGat                                                 <  2:461104/139‑1 (MQ=255)

                                                                            aaaaaaGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGata                                                <  1:85504/139‑1 (MQ=255)

                                                                                   aagCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTc                                         <  1:748422/139‑1 (MQ=255)

                                                                                     gCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCtt                                           <  1:37178/135‑1 (MQ=255)

                                                                                     gCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCtt                                           >  2:37178/1‑135 (MQ=255)

                                                                                         aGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGt                                                                                                                                 <  2:413087/45‑1 (MQ=255)

                                                                                         aGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGt                                                                                                                                 >  1:413087/1‑45 (MQ=255)

                                                                                                        ccGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGa                                                                                    >  2:80994/1‑75 (MQ=255)

                                                                                                        ccGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGa                                                                                    <  1:80994/75‑1 (MQ=255)

                                                                                                            cGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCATAGAACATAACGGCACTTCGCTGTCGGATGCTATTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCct                <  2:500863/139‑1 (MQ=255)

                                                                                                            cGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCct                >  1:303885/1‑139 (MQ=255)

                                                                                                               aTCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGat                                                 <  2:505226/103‑1 (MQ=255)

                                                                                                               aTCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGat                                                 >  1:505226/1‑103 (MQ=255)

                                                                                                                tCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgact            <  2:590738/139‑1 (MQ=255)

                                                                                                                  aCCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgactga          <  2:629256/139‑1 (MQ=255)

                                                                                                                          aaCGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATc  <  2:409573/139‑1 (MQ=255)

ACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATC  >  NC_000913/2629352‑2629612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A