Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I2 R1 757 38.1 1607544 96.5% 1551279 117.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,293,196 IS5 (+) +4 bp intergenic (‑274/‑328) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1293196 =0 (0.000)48 (1.240) 36/232 0.1 100% intergenic (‑274/‑331) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 12931990 (0.000)41 (1.060) 34/232 0.2 100% intergenic (‑277/‑328) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 1299499 = NA (NA)noncoding (1/1195 nt) IS5 repeat region

TTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGTGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293327‑1293196
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT  <  NC_000913/1300693‑1300557
                                                                                                                                                                                                                                                                             
TTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGTGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGT                                                                                                                                    <  1:776837/139‑1
 TTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGTGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGA                                                                                                                                        >  2:235760/1‑134
 TTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGTGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGA                                                                                                                                        <  1:235760/134‑1
              CAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGTGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGG                                                                                                                      >  1:645444/1‑139
                            CTGTTGACCTCCAGGAGATAGTGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGC                                                                                                        >  1:303863/1‑139
                            CTGTTGACCTCCAGGAGATAGTGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGC                                                                                                        <  1:542880/139‑1
                                               AGTGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTT                                                                                     >  1:338356/1‑139
                                                 TGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCT                                                                                   >  1:145628/1‑139
                                                 TGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCT                                                                                   >  1:524395/1‑139
                                                      TACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATG                                                                              >  1:447121/1‑139
                                                           AGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTATGGA                                                                                                                                        <  1:517628/76‑1
                                                           AGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAG                                                                                                                           >  1:734462/1‑89
                                                           AGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAG                                                                                                                           <  2:734462/89‑1
                                                           AGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGA                                                                                                                                        >  2:517628/1‑76
                                                               CATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCG                                                                     <  1:137948/139‑1
                                                                ATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGA                                                                    >  1:521298/1‑139
                                                                ATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGA                                                                    >  2:328753/1‑139
                                                                ATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCAT                                                                                            <  2:290499/115‑1
                                                                ATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCAT                                                                                            >  1:290499/1‑115
                                                                 TGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAT                                                                   >  1:488605/1‑139
                                                                 TGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAT                                                                   <  2:131042/139‑1
                                                                  GCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATT                                                                  >  2:564422/1‑139
                                                                  GCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAC                                                                                                    >  2:528459/1‑105
                                                                  GCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAC                                                                                                    <  1:528459/105‑1
                                                                       TATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTC                                                             <  2:415901/139‑1
                                                                       TATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTC                                                             >  2:197629/1‑139
                                                                              ACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTA                                                      >  1:359994/1‑139
                                                                              ACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTA                                                      >  2:189909/1‑139
                                                                               CTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAA                                                     <  2:359994/139‑1
                                                                                TGTTCTACTTTTCATCATTCGCTTAATAGGAAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTCCTACATGTTTGAGACGATTTTTTCTCCCGTAAA                                                    >  1:422258/1‑139
                                                                                  TTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATG                                                  >  1:33242/1‑139
                                                                                  TTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATG                                                  >  1:156407/1‑139
                                                                                    CTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCC                                                <  2:524395/139‑1
                                                                                         TTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAA                                           <  1:197629/139‑1
                                                                                         TTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAA                                           <  2:86907/139‑1
                                                                                         TTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAA                                           <  2:798151/139‑1
                                                                                          TTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT                                          <  2:101504/139‑1
                                                                                            CATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCA                                        >  1:347097/1‑139
                                                                                              TCAGTCGCTTACTAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGC                                      <  2:521693/139‑1
                                                                                               CATTCGCTTAATAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCC                                     <  2:338356/139‑1
                                                                                                          TAGGGAATTCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCG                          >  2:768170/1‑139
                                                                                                                  TCTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCG                  <  1:787712/139‑1
                                                                                                                   CTCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGC                 <  1:189909/139‑1
                                                                                                                    TCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAC                                                                                                    <  2:525902/55‑1
                                                                                                                    TCGTAAACACAACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAC                                                                                                    >  1:525902/1‑55
                                                                                                                       TAAACACAACTCAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGA‑TTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTT            <  2:521298/139‑1
                                                                                                                              AACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCG      >  1:447943/1‑139
                                                                                                                                  AAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT  <  1:356543/139‑1
                                                                                                                                                                                                                                                                             
TTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGTGCAATACTAAGTCCATGCTCTTATTGCGACTGTTCTACTTTTCATCATTCGCTTAATAGGGAATTCTCGTAAACACAACTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293327‑1293196
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT  <  NC_000913/1300693‑1300557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A