Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,491,878	G→A	intergenic (+28/+44)	ydcA → / ← hokB	putative periplasmic protein/toxic polypeptide, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,491,878	0	G	A	100.0%	112.6 / NA	36	intergenic (+28/+44)	ydcA/hokB	putative periplasmic protein/toxic polypeptide, small
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/18); total (18/18)

AAACCCTGTTCTGGTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGT  >  NC_000913/1491743‑1492002

aaaCCCTGTTCTGGTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAaaataaat                                                                                                                           >  2:462506/1‑139 (MQ=255)

                 gAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATgcgc                                                                                                          <  2:560027/139‑1 (MQ=255)

                  aaaGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGcgcg                                                                                                         <  1:554406/139‑1 (MQ=255)

                               cacaCTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCcg                                                                                            <  1:462506/139‑1 (MQ=255)

                                   cTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCt                                                                                        <  2:559290/139‑1 (MQ=255)

                                      tACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGc                                                                                     >  2:359651/1‑139 (MQ=255)

                                      tACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGc                                                                                     >  1:380729/1‑139 (MQ=255)

                                      tACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGc                                                                                     >  1:487686/1‑139 (MQ=255)

                                      tACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGc                                                                                     >  1:770468/1‑139 (MQ=255)

                                                ggcAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTgg                                                                           >  2:333221/1‑139 (MQ=255)

                                                ggcAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTgg                                                                           <  2:23695/139‑1 (MQ=255)

                                                                   atgGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACg                                                                        <  2:742992/123‑1 (MQ=255)

                                                                   atgGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACg                                                                        >  1:742992/1‑123 (MQ=255)

                                                                       ttCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagca                                                    <  1:490888/139‑1 (MQ=255)

                                                                        tCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCaa                                                   >  2:84214/1‑139 (MQ=255)

                                                                         cTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAc                                                  <  2:418075/139‑1 (MQ=255)

                                                                          tATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAcc                                                 <  2:487686/139‑1 (MQ=255)

                                                                           aTTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAcct                                                >  2:108188/1‑139 (MQ=255)

                                                                                 gCATCGATAAAAACATGCACTAACAGAAGTGTAAAAGGGGCGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTAt                                          >  2:490888/1‑139 (MQ=255)

                                                                                 gCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGACCGCAGCAAc                                                  <  1:585635/131‑1 (MQ=255)

                                                                                 gCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGACCGCAGCAAc                                                  >  2:585635/1‑131 (MQ=255)

                                                                                  cATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATc                                         >  1:205310/1‑139 (MQ=255)

                                                                                   aTCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCa                                        >  2:662937/1‑139 (MQ=255)

                                                                                       aaaaaaaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTgg                                                                           >  1:603197/1‑100 (MQ=255)

                                                                                       aaaaaaaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTgg                                                                           <  2:603197/100‑1 (MQ=255)

                                                                                                            aGTGTAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTaga               >  1:791334/1‑139 (MQ=255)

                                                                                                               gtAAAAGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCg            >  1:770487/1‑139 (MQ=255)

                                                                                                                 aaaaGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGtt          >  1:355714/1‑139 (MQ=255)

                                                                                                                    aGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGt       <  2:355714/139‑1 (MQ=255)

                                                                                                                    aGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGt       <  2:770468/139‑1 (MQ=255)

                                                                                                                    aGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGt       <  2:791334/139‑1 (MQ=255)

                                                                                                                    aGGGGTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGt       <  1:35039/139‑1 (MQ=255)

                                                                                                                     ggggTGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTc      >  1:320323/1‑139 (MQ=255)

                                                                                                                         tGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGt  <  2:205310/139‑1 (MQ=255)

                                                                                                                         tGCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGt  <  1:662937/139‑1 (MQ=255)

                                                                                                                          gCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTg                                                                      >  2:659510/1‑70 (MQ=255)

                                                                                                                          gCCATGAGAAAATAAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTg                                                                      <  1:659510/70‑1 (MQ=255)

AAACCCTGTTCTGGTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGT  >  NC_000913/1491743‑1492002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A