Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
757 |
38.1 |
1607544 |
96.5% |
1551279 |
117.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,763,794 |
T→C |
E72E (GAA→GAG) |
sufB ← |
component of SufBCD Fe‑S cluster assembly scaffold |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,763,794 | 0 | T | C | 100.0%
| 95.2
/ NA
| 27 | E72E (GAA→GAG) | sufB | component of SufBCD Fe‑S cluster assembly scaffold |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/13); total (14/13) |
GTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGTTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGG > NC_000913/1763662‑1763929
|
gTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTcc > 1:186882/1‑139 (MQ=255)
tgcACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATgcgc < 2:13767/139‑1 (MQ=255)
cgcgCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAg > 2:399139/1‑139 (MQ=255)
cgcCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGc > 1:331907/1‑139 (MQ=255)
cgcCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGc > 2:438192/1‑139 (MQ=255)
aGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGa < 2:186882/139‑1 (MQ=255)
gcAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGAt < 2:451115/119‑1 (MQ=255)
gcAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGAt > 1:451115/1‑119 (MQ=255)
gcAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTcc < 2:134270/139‑1 (MQ=255)
tgtCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCAt < 2:191122/139‑1 (MQ=255)
gtcACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGATTAGACGAAACTCCAGCATCCAc > 2:153746/1‑139 (MQ=255)
cGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGc > 2:232765/1‑139 (MQ=255)
gCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCg < 1:399139/139‑1 (MQ=255)
agtagtAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAAt > 2:192054/1‑139 (MQ=255)
gtagCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTgcg < 1:438192/139‑1 (MQ=255)
gTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGcacc < 1:192054/139‑1 (MQ=255)
tCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTcc < 1:212456/41‑1 (MQ=255)
tCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTcc > 2:212456/1‑41 (MQ=255)
cTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTgcg < 2:373855/115‑1 (MQ=255)
cTTGTCGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTgcg > 1:373855/1‑115 (MQ=255)
cGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACg < 2:17720/94‑1 (MQ=255)
cGTAGTGCGCTTTCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACg > 1:17720/1‑94 (MQ=255)
tCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCAt < 1:652210/135‑1 (MQ=255)
tCAACCAGTGCGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCAt > 2:652210/1‑135 (MQ=255)
cGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGc > 1:431042/1‑117 (MQ=255)
cGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGc < 2:431042/117‑1 (MQ=255)
cGGCTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTgg > 1:393432/1‑139 (MQ=255)
|
GTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAGCTGTAATCCTGATAATTCAGCTTGTCGTAGTGCGCTTTCAACCAGTGCGGTTCTTCCATCTCCAGCCATGCGCGATAGGCGTTTAGACGAAACTCCAGCATCCACTCCGGCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGG > NC_000913/1763662‑1763929
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
