Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
757 |
38.1 |
1607544 |
96.5% |
1551279 |
117.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,850,810 |
(C)5→4 |
intergenic (+117/‑50) |
sppA → / → ansA |
protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,850,806 | 0 | C | . | 100.0%
| 147.2
/ NA
| 35 | intergenic (+113/‑54) | sppA/ansA | protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (18/17); total (18/17) |
CGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG > NC_000913/1850678‑1850940
|
cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCa < 1:320236/139‑1 (MQ=255)
cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCa > 1:238092/1‑139 (MQ=255)
cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGATGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCTCTcccctgc > 1:521276/1‑126 (MQ=255)
cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccctgc < 2:521276/129‑4 (MQ=255)
aGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGc < 2:802327/139‑1 (MQ=255)
tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGct < 2:620085/135‑1 (MQ=255)
tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGct > 1:620085/1‑135 (MQ=255)
tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCttt > 1:71323/1‑139 (MQ=255)
tACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCg > 2:501612/1‑139 (MQ=255)
aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc > 2:412005/1‑139 (MQ=255)
gTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtca < 1:722946/139‑1 (MQ=255)
ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt < 1:476855/139‑1 (MQ=255)
gCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattc < 2:238092/139‑1 (MQ=255)
gCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTccc > 1:599474/1‑85 (MQ=255)
gCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTccc < 2:599474/85‑1 (MQ=255)
aCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAttt > 2:386437/1‑139 (MQ=255)
aCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAttt > 1:337233/1‑139 (MQ=255)
cgacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGt < 1:412005/139‑1 (MQ=255)
cGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcggg < 2:462963/139‑1 (MQ=255)
ttCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATc > 2:271915/1‑139 (MQ=255)
aTCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg < 1:271915/139‑1 (MQ=255)
aTCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg < 2:406325/139‑1 (MQ=255)
tCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGaa > 1:270423/1‑86 (MQ=255)
tCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGaa < 2:270423/86‑1 (MQ=255)
cATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAggg > 2:551471/1‑139 (MQ=255)
aTAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAATAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGt > 1:575222/1‑139 (MQ=255)
aTAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGt > 2:452935/1‑139 (MQ=255)
tATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCttt > 1:705075/1‑44 (MQ=255)
tATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCttt < 2:705075/44‑1 (MQ=255)
tCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGata < 1:334772/47‑1 (MQ=255)
tCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGata > 2:334772/1‑47 (MQ=255)
tCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTAc < 1:741244/85‑1 (MQ=255)
tCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTAc > 2:741244/1‑85 (MQ=255)
cAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt > 1:7614/1‑51 (MQ=255)
cAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt < 2:7614/51‑1 (MQ=255)
cAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc < 2:337233/139‑1 (MQ=255)
gcgcT‑CCCCTGCCTCACGTATATACTTTTGCTCttt > 2:428066/1‑36 (MQ=255)
gcgcT‑CCCCTGCCTCACGTATATACTTTTGCTCttt < 1:428066/36‑1 (MQ=255)
gcgcT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg > 1:250488/1‑139 (MQ=255)
|
CGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG > NC_000913/1850678‑1850940
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
