Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
757 |
38.1 |
1607544 |
96.5% |
1551279 |
117.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,117,123 |
G→A |
R34C (CGC→TGC) |
glpK ← |
glycerol kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,117,123 | 0 | G | A | 100.0%
| 93.8
/ NA
| 30 | R34C (CGC→TGC) | glpK | glycerol kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/15); total (15/15) |
CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAA > NC_000913/4116987‑4117254
|
ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACt > 1:530562/1‑139 (MQ=255)
aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCATGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg > 2:125470/1‑139 (MQ=255)
gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca > 1:376261/1‑139 (MQ=255)
tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCATGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg < 1:125470/139‑1 (MQ=255)
gAACTGATATCAGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTgg < 1:151397/139‑1 (MQ=255)
tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTAc > 2:80683/1‑139 (MQ=255)
ccAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc < 1:715021/139‑1 (MQ=255)
aGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa < 2:800363/114‑1 (MQ=255)
aGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa > 1:800363/1‑114 (MQ=255)
cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg > 2:252719/1‑139 (MQ=255)
gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTGGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg > 1:574413/1‑139 (MQ=255)
tGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa < 1:347693/85‑1 (MQ=255)
tGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa > 2:347693/1‑85 (MQ=255)
gCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg < 1:20412/139‑1 (MQ=255)
cAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa < 2:417746/139‑1 (MQ=255)
ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg < 1:186171/97‑1 (MQ=255)
ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg > 2:186171/1‑97 (MQ=255)
ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCt > 1:730925/1‑103 (MQ=255)
ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCt < 2:730925/103‑1 (MQ=255)
ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTc < 2:299203/118‑1 (MQ=255)
ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTc > 1:299203/1‑118 (MQ=255)
tCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttttt > 2:175721/1‑139 (MQ=255)
aaCCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa < 2:574413/139‑1 (MQ=255)
ggTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTcc > 2:796322/1‑139 (MQ=255)
ggTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGCGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTc > 2:510369/1‑139 (MQ=255)
tttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCg < 2:376261/139‑1 (MQ=255)
tGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTaa > 1:802609/1‑38 (MQ=255)
tGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTaa < 2:802609/38‑1 (MQ=255)
tGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAg < 1:796322/139‑1 (MQ=255)
aaTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGaa < 2:33924/139‑1 (MQ=255)
|
CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAA > NC_000913/4116987‑4117254
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
