Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 773 79.9 4112934 92.2% 3792125 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 852,869 C→T *43* (TAG→TAA)  mntS ← Mn(2)‑response protein, MntR‑repressed

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913852,8690CT100.0% 273.5 / NA 84*43* (TAG→TAA) mntSMn(2)‑response protein, MntR‑repressed
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/48);  total (36/48)

CAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGA  >  NC_000913/852741‑853001
                                                                                                                                |                                                                                                                                    
cAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCgg                                                                                                                            <  1:992992/139‑1 (MQ=255)
cAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCgg                                                                                                                            <  1:1309718/139‑1 (MQ=255)
cAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCgg                                                                                                                            <  1:1897885/139‑1 (MQ=255)
cAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCgg                                                                                                                            <  2:1951740/139‑1 (MQ=255)
cAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCCTACCGCGACGCCGTTATTTATCgg                                                                                                                            <  1:1395801/139‑1 (MQ=255)
   gTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCgg                                                                                                                            <  2:815753/136‑1 (MQ=255)
   gTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCgg                                                                                                                            >  1:815753/1‑136 (MQ=255)
   gTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAg                                                                                                                         >  2:700482/1‑139 (MQ=255)
    tCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATGCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGCAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAgg                                                                                                                        >  2:1376811/1‑139 (MQ=255)
     cAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGt                                                                                                                       <  2:820985/139‑1 (MQ=255)
     cAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGt                                                                                                                       <  1:468236/139‑1 (MQ=255)
        ctactGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTa                                                                                                                    >  2:240682/1‑139 (MQ=255)
        ctactGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTa                                                                                                                    >  2:1473945/1‑139 (MQ=255)
                 aaCAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTtgctgc                                                                                                           >  1:419094/1‑139 (MQ=255)
                   cAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCgg                                                                                                         >  2:660657/1‑139 (MQ=255)
                    aaaTCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGt                                                                                                        <  2:1124234/139‑1 (MQ=255)
                     aaTCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGtt                                                                                                       <  1:240682/139‑1 (MQ=255)
                         ttttGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTttgtt                                                                                                   <  1:700482/139‑1 (MQ=255)
                           ttGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgt                                                                                                 >  2:1505386/1‑139 (MQ=255)
                              cGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGa                                                                                              <  2:419094/139‑1 (MQ=255)
                                aaGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGt                                                                                                        >  1:561135/1‑127 (MQ=255)
                                aaGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGt                                                                                                        <  2:561135/127‑1 (MQ=255)
                                aaGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGAcc                                                                                            >  2:1293881/1‑139 (MQ=255)
                                 aGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCa                                                                                           <  2:1174867/139‑1 (MQ=255)
                                  ggggATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCat                                                                                          >  1:1773240/1‑139 (MQ=255)
                                   gggATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCata                                                                                         >  2:885533/1‑139 (MQ=255)
                                       ttGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     <  1:1069260/139‑1 (MQ=255)
                                       ttGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGAGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     <  1:1293881/139‑1 (MQ=255)
                                        tGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                    <  1:1266586/139‑1 (MQ=255)
                                        tGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     >  2:622875/1‑138 (MQ=255)
                                        tGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     <  1:622875/138‑1 (MQ=255)
                                        tGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGCAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                    <  1:1473945/139‑1 (MQ=255)
                                               ccGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTa                                                                                                                                >  1:2008321/1‑88 (MQ=255)
                                               ccGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTa                                                                                                                                <  2:2008321/88‑1 (MQ=255)
                                               ccGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                    >  1:1117485/1‑132 (MQ=255)
                                               ccGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                    <  2:1117485/132‑1 (MQ=255)
                                               ccGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATa                                                                             <  1:1376811/139‑1 (MQ=255)
                                                cGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGt                                                                                                        >  1:1459391/1‑111 (MQ=255)
                                                cGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGt                                                                                                        <  2:1459391/111‑1 (MQ=255)
                                                    aaaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATagagag                                                                        <  1:660657/139‑1 (MQ=255)
                                                    aaaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATagagag                                                                        <  1:885533/139‑1 (MQ=255)
                                                            gggTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATagagag                                                                        >  1:1392503/1‑131 (MQ=255)
                                                            gggTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATagagag                                                                        <  2:1392503/131‑1 (MQ=255)
                                                             ggTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                    >  1:726469/1‑118 (MQ=255)
                                                             ggTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                    <  2:726469/118‑1 (MQ=255)
                                                             ggTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTaa                                                               <  2:472429/139‑1 (MQ=255)
                                                                 tttCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     <  2:248671/113‑1 (MQ=255)
                                                                 tttCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     >  1:248671/1‑113 (MQ=255)
                                                                  ttCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCg                                                                                      <  1:1908837/111‑1 (MQ=255)
                                                                  ttCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCg                                                                                      >  2:1908837/1‑111 (MQ=255)
                                                                  ttCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGcaca                                                                                  <  2:152841/115‑1 (MQ=255)
                                                                  ttCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGcaca                                                                                  >  1:152841/1‑115 (MQ=255)
                                                                         cAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTaaa                                                   <  2:1773240/139‑1 (MQ=255)
                                                                         cAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTaaa                                                   <  2:449644/139‑1 (MQ=255)
                                                                               gcgTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGgaga                                             <  2:615510/139‑1 (MQ=255)
                                                                                     tGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     <  1:290545/93‑1 (MQ=255)
                                                                                     tGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     >  2:290545/1‑93 (MQ=255)
                                                                                          gCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAATGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATACAGAGCAGCATTAACCGTACTTTAAAGGGAGAAAGACTAAaca                                  <  1:200770/139‑1 (MQ=255)
                                                                                          gCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAaca                                  <  1:1505386/139‑1 (MQ=255)
                                                                                          gCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAaca                                  >  2:1572834/1‑139 (MQ=255)
                                                                                                     ggAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATacac                       >  2:1779608/1‑139 (MQ=255)
                                                                                                     ggAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATacac                       <  1:1572834/139‑1 (MQ=255)
                                                                                                      gAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                    >  1:1953723/1‑77 (MQ=255)
                                                                                                      gAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                    <  2:1953723/77‑1 (MQ=255)
                                                                                                      gAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACAcc                      >  1:1417137/1‑139 (MQ=255)
                                                                                                           aaGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAgcagca                                                                   >  2:2045289/1‑89 (MQ=255)
                                                                                                           aaGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAgcagca                                                                   <  1:2045289/89‑1 (MQ=255)
                                                                                                             gcggcgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGaa               <  1:103891/139‑1 (MQ=255)
                                                                                                              cggcgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCATAACATAGAGAGCAGCATTAACCGTACtt                                                       <  2:1272105/98‑1 (MQ=255)
                                                                                                              cggcgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACtt                                                       >  1:1272105/1‑98 (MQ=255)
                                                                                                                gcgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtc            >  1:1506064/1‑139 (MQ=255)
                                                                                                                 cgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTtgctg                                                                                                            >  1:1651264/1‑42 (MQ=255)
                                                                                                                 cgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTtgctg                                                                                                            <  2:1651264/42‑1 (MQ=255)
                                                                                                                 cgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATc                                                                                       >  1:1828395/1‑63 (MQ=255)
                                                                                                                 cgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATc                                                                                       <  2:1828395/63‑1 (MQ=255)
                                                                                                                        cgACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     >  1:570610/1‑58 (MQ=255)
                                                                                                                        cgACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                     <  2:570610/58‑1 (MQ=255)
                                                                                                                        cgACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAacac                                 <  2:255859/110‑1 (MQ=255)
                                                                                                                        cgACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAacac                                 >  1:255859/1‑110 (MQ=255)
                                                                                                                          aCGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGtt                                                                                                       <  2:1347025/38‑1 (MQ=255)
                                                                                                                          aCGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAgg                                                 <  1:293844/92‑1 (MQ=255)
                                                                                                                          aCGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAgg                                                 >  2:293844/1‑92 (MQ=255)
                                                                                                                          aCGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGa  >  1:1656491/1‑139 (MQ=255)
                                                                                                                          aCGCCGTAATTTATCGGAAGGTTTATCTTGCTGCGGtt                                                                                                       >  1:1347025/1‑38 (MQ=255)
                                                                                                                                |                                                                                                                                    
CAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGA  >  NC_000913/852741‑853001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A