Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
773 |
79.9 |
4112934 |
92.2% |
3792125 |
104.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,974,824 |
C→T |
L167L (CTG→CTA) |
cheA ← |
fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,974,824 | 0 | C | T | 100.0%
| 174.0
/ NA
| 53 | L167L (CTG→CTA) | cheA | fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/23); total (30/23) |
TGATGTCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCA > NC_000913/1974700‑1974957
|
tgATGTCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGa < 1:1169449/133‑1 (MQ=255)
tgATGTCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGa > 2:1169449/1‑133 (MQ=255)
tgATGTCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGAt > 2:575246/1‑139 (MQ=255)
aTGTCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATaa > 2:2056152/1‑139 (MQ=255)
cATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATtcg < 1:1317390/139‑1 (MQ=255)
tGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGa < 1:1036378/115‑1 (MQ=255)
tGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGa > 2:1036378/1‑115 (MQ=255)
cGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGa < 1:643845/139‑1 (MQ=255)
gagaGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCtt > 2:934325/1‑139 (MQ=255)
agagCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATtcgtcg < 2:984119/109‑1 (MQ=255)
agagCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATtcgtcg > 1:984119/1‑109 (MQ=255)
gagCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCgact < 1:1796053/116‑1 (MQ=255)
gagCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCgact > 2:1796053/1‑116 (MQ=255)
aTCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCa > 1:693726/1‑139 (MQ=255)
ccccTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACtttt > 2:660776/1‑139 (MQ=255)
ttCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCaa > 1:545758/1‑139 (MQ=255)
tCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAAc < 2:1649814/139‑1 (MQ=255)
cacGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACcaca > 2:680771/1‑139 (MQ=255)
acGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGAc > 2:189702/1‑103 (MQ=255)
acGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGAc < 1:189702/103‑1 (MQ=255)
cAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTaa > 2:426894/1‑139 (MQ=255)
cAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTaa > 1:930397/1‑139 (MQ=255)
tCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGc > 1:2028819/1‑139 (MQ=255)
aTGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGAt < 2:545758/139‑1 (MQ=255)
tCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGcgcg > 2:1923080/1‑47 (MQ=255)
tCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGcgcg < 1:1923080/47‑1 (MQ=255)
tCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGc > 1:325292/1‑139 (MQ=255)
cAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGt < 2:310960/94‑1 (MQ=255)
cAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGt > 1:310960/1‑94 (MQ=255)
cttcCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGAc > 1:1673691/1‑69 (MQ=255)
cttcCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGAc < 2:1673691/69‑1 (MQ=255)
cttcCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCttt > 1:1030590/1‑139 (MQ=255)
cttcCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCttt > 1:729728/1‑139 (MQ=255)
tcCAGCAGGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCg < 2:729728/139‑1 (MQ=255)
agcagGTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCtt > 2:1629053/1‑139 (MQ=255)
gTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGt > 1:167853/1‑76 (MQ=255)
gTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGt < 2:167853/76‑1 (MQ=255)
gTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGAt < 1:582507/115‑1 (MQ=255)
gTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGAt > 2:582507/1‑115 (MQ=255)
gTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAAt > 2:1406999/1‑139 (MQ=255)
gTCGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAAt > 1:1941928/1‑139 (MQ=255)
cGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGc < 2:2028819/139‑1 (MQ=255)
cGACTTCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGc < 1:680771/139‑1 (MQ=255)
ttCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGt > 2:191221/1‑139 (MQ=255)
ttCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGt < 2:930397/139‑1 (MQ=255)
tCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGtt > 1:1429405/1‑70 (MQ=255)
tCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGtt < 2:1429405/70‑1 (MQ=255)
tCCCCGGCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGtt < 1:934325/139‑1 (MQ=255)
ggCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAAc < 1:527106/78‑1 (MQ=255)
ggCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAAc < 1:1992756/78‑1 (MQ=255)
ggCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAAc > 2:527106/1‑78 (MQ=255)
ggCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAAc > 2:1992756/1‑78 (MQ=255)
gCCTTTAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCa < 1:1629053/139‑1 (MQ=255)
|
TGATGTCATCTTCGGCGATGTCGCCCGGTAATATTGCCGAGAGCGAATCCGCCCCTTTCACCACGTCAGTTAACGTTGTCAGATGTCCCAGTTCTTCTTCCAGCAGGTCGACTTCCCCGGCCTTCAGGCGCGAAAGGATAATTCGTCGCGGCGACTGACTGCGACTCTGCTCATCTTGCGGTTCACTTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCA > NC_000913/1974700‑1974957
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
