Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,131,667	T→C	D135G (GAC→GGC)	wcaC ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,131,667	0	T	C	100.0%	189.9 / NA	54	D135G (GAC→GGC)	wcaC	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/27); total (27/27)

TGCCCGCCACCAGTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGTCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGT  >  NC_000913/2131533‑2131797

tGCCCGCCACCAGTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTg                                                                                                                                >  1:268564/1‑139 (MQ=255)

            gTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTccagac                                                                                                                    >  2:1295614/1‑139 (MQ=255)

                      gcgATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACg                                                                                                          >  2:737692/1‑139 (MQ=255)

                                  cACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATgg                                                                                              <  2:268564/139‑1 (MQ=255)

                                  cACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATgg                                                                                              >  1:1850144/1‑139 (MQ=255)

                                        cggATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGt                                                                                                         <  1:765270/122‑1 (MQ=255)

                                        cggATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGt                                                                                                         >  2:765270/1‑122 (MQ=255)

                                        cggATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTc                                                                                        >  2:621888/1‑139 (MQ=255)

                                          gATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCAc                                                                                      >  1:823555/1‑139 (MQ=255)

                                                       ggTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAg                                                                         >  1:1423942/1‑139 (MQ=255)

                                                        gTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAgc                                                                        >  1:1724624/1‑139 (MQ=255)

                                                         tCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                              >  2:1480525/1‑84 (MQ=255)

                                                         tCGGGCAGTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                              <  1:1480525/84‑1 (MQ=255)

                                                           gggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGt                                                                                                         <  2:1650342/103‑1 (MQ=255)

                                                           gggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGt                                                                                                         >  1:1650342/1‑103 (MQ=255)

                                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                              <  1:1733716/81‑1 (MQ=255)

                                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                              >  2:1733716/1‑81 (MQ=255)

                                                            ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcac                                                                    >  1:1286694/1‑139 (MQ=255)

                                                             gCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacc                                                                   <  1:283259/139‑1 (MQ=255)

                                                              cATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGGGTCCGGTAACACTCCAGTGGCCGCGCAGCGTCCAGACCATAGTGACGTCCTGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                  <  2:1308974/139‑1 (MQ=255)

                                                               aTTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcaccac                                                                 <  2:823555/139‑1 (MQ=255)

                                                                tttCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACg                                                                <  1:1295614/139‑1 (MQ=255)

                                                                     ggCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCtt                                                           <  2:1850144/139‑1 (MQ=255)

                                                                     ggCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCtt                                                           <  1:621888/139‑1 (MQ=255)

                                                                           cTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGTACCACGCTCTTAAGAtt                                                     <  2:1423942/139‑1 (MQ=255)

                                                                           cTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGAtt                                                     <  2:1286694/139‑1 (MQ=255)

                                                                                ttCCAGCCTTGGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCAGGTTTATGGTTTTTCACATTGTCGCAAAAGCGCACCACGCTCTTAAGATTTAGcc                                                <  1:2036509/139‑1 (MQ=255)

                                                                                        ttCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCtg                                        >  2:1587853/1‑139 (MQ=255)

                                                                                          cGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCtgtg                                      <  1:737692/139‑1 (MQ=255)

                                                                                                cGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                  >  2:1346836/1‑105 (MQ=255)

                                                                                                cGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                  <  1:1346836/105‑1 (MQ=255)

                                                                                                    ggTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCtt                                                           <  2:1237702/108‑1 (MQ=255)

                                                                                                    ggTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCtt                                                           >  1:1237702/1‑108 (MQ=255)

                                                                                                    ggTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGcc                                                >  1:477955/1‑119 (MQ=255)

                                                                                                    ggTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGcc                                                <  2:477955/119‑1 (MQ=255)

                                                                                                            cgcAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTccagac                                                                                                                    >  2:389076/1‑43 (MQ=255)

                                                                                                            cgcAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTccagac                                                                                                                    <  1:389076/43‑1 (MQ=255)

                                                                                                                gCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACtt                                                                                    >  2:984648/1‑71 (MQ=255)

                                                                                                                gCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACtt                                                                                    <  1:984648/71‑1 (MQ=255)

                                                                                                                gCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                  <  2:1377753/89‑1 (MQ=255)

                                                                                                                gCGTCCGGTAACAATCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                  >  1:1377753/1‑89 (MQ=255)

                                                                                                                  gTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGaccac              <  1:1587853/139‑1 (MQ=255)

                                                                                                                             cTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCtg   >  1:1134026/1‑139 (MQ=255)

                                                                                                                              tCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTc                                                                                                        >  1:1094011/1‑37 (MQ=255)

                                                                                                                              tCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTc                                                                                                        <  2:1094011/37‑1 (MQ=255)

                                                                                                                              tCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCtt                                                           >  2:187656/1‑82 (MQ=255)

                                                                                                                              tCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCtt                                                           <  1:187656/82‑1 (MQ=255)

                                                                                                                              tCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCtgt  >  2:1909026/1‑139 (MQ=255)

                                                                                                                              tCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCtgt  >  2:210197/1‑139 (MQ=255)

                                                                                                                              tCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCtgt  <  2:1134026/139‑1 (MQ=255)

                                                                                                                                cAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                  <  2:1746779/73‑1 (MQ=255)

                                                                                                                                cAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                  >  1:1746779/1‑73 (MQ=255)

                                                                                                                                cAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGa                            >  2:1072436/1‑111 (MQ=255)

                                                                                                                                cAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGa                            <  1:1072436/111‑1 (MQ=255)

TGCCCGCCACCAGTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGTCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGT  >  NC_000913/2131533‑2131797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A