Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,447,320	T→C	T52A (ACA→GCA)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,447,320	0	T	C	100.0%	163.2 / NA	46	T52A (ACA→GCA)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/17); total (29/17)

CTGTAATCCTGAGAGCGCTGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGTCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTT  >  NC_000913/2447187‑2447451

cTGTAATCCTGAGAGCGCTGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAg                                                                                                                                >  2:1190749/1‑139 (MQ=255)

   tAATCCTGAGAGCGCTGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCAGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGc                                                                                                                             <  1:1949668/139‑1 (MQ=255)

       ccTGAGAGCGCTGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGcgacg                                                                                                                         <  1:955441/139‑1 (MQ=255)

             agCGCTGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGccc                                                                                                                                   >  2:2019862/1‑123 (MQ=255)

             agCGCTGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGccc                                                                                                                                   <  1:2019862/123‑1 (MQ=255)

             agCGCTGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTc                                                                                                                   >  2:1756823/1‑139 (MQ=255)

                 cTGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAgg                                                                                                               >  1:1902154/1‑139 (MQ=255)

                  tGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGt                                                                                                              >  2:703636/1‑139 (MQ=255)

                  tGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGt                                                                                                              >  1:996965/1‑139 (MQ=255)

                       aGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGt                                                                                                         >  1:918789/1‑139 (MQ=255)

                              tCGATCAACAAGCCAATGCTGGTGCCGGTTGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGt                                                                                                  <  2:1391035/139‑1 (MQ=255)

                                   caacaaGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCt                                                                                             >  2:171511/1‑139 (MQ=255)

                                                  ggTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATg                                                                              <  2:996965/139‑1 (MQ=255)

                                                   gTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGc                                                                             >  1:22512/1‑139 (MQ=255)

                                                   gTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGc                                                                             >  1:1713405/1‑139 (MQ=255)

                                                    tGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGcc                                                                            <  1:1756823/139‑1 (MQ=255)

                                                        ggTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGa                                                                                 <  2:1595376/130‑1 (MQ=255)

                                                        ggTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGa                                                                                 >  1:1595376/1‑130 (MQ=255)

                                                        ggTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGc                                                                        <  2:1902154/139‑1 (MQ=255)

                                                             taACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGGTCCGGATCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCAGGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAac                                                                   >  2:1375617/1‑139 (MQ=255)

                                                                    ttCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTg                                                                                                       >  1:763935/1‑96 (MQ=255)

                                                                    ttCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTg                                                                                                       <  2:763935/96‑1 (MQ=255)

                                                                     tCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATcga                                                           >  2:577645/1‑139 (MQ=255)

                                                                             acCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAg                                                   >  1:1284049/1‑139 (MQ=255)

                                                                             acCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAg                                                   >  1:1221269/1‑139 (MQ=255)

                                                                               cGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGcc                                                                            <  2:89878/112‑1 (MQ=255)

                                                                               cGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGcc                                                                            >  1:89878/1‑112 (MQ=255)

                                                                               cGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGcc                                                 >  1:1535787/1‑139 (MQ=255)

                                                                               cGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGcc                                                 <  2:1713405/139‑1 (MQ=255)

                                                                                            gACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCg                                    >  1:1756587/1‑139 (MQ=255)

                                                                                                            cggcgCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCg                                                                                                                  >  1:382425/1‑45 (MQ=255)

                                                                                                            cggcgCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCg                                                                                                                  <  2:382425/45‑1 (MQ=255)

                                                                                                            cggcgCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAgg                    >  1:39535/1‑139 (MQ=255)

                                                                                                            cggcgCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAgg                    >  1:261678/1‑139 (MQ=255)

                                                                                                            cggcgCTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAgg                    >  2:949011/1‑139 (MQ=255)

                                                                                                                 cTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGtt               >  1:199111/1‑139 (MQ=255)

                                                                                                                 cTGGGTGGTATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGtt               <  2:918789/139‑1 (MQ=255)

                                                                                                                  tGGGTGGTATAGCGCGATGCCCCAGGGCGACGACgg                                                                                                                     <  2:595524/36‑1 (MQ=255)

                                                                                                                  tGGGTGGTATAGCGCGATGCCCCAGGGCGACGACgg                                                                                                                     >  1:595524/1‑36 (MQ=255)

                                                                                                                    ggtggtATAGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTAcg            <  1:1190749/139‑1 (MQ=255)

                                                                                                                         tataGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGaaa       >  2:1898591/1‑139 (MQ=255)

                                                                                                                            aGCGCGATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAagag    <  1:1815691/139‑1 (MQ=255)

                                                                                                                              cgcgATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAagag    <  1:1326036/137‑1 (MQ=255)

                                                                                                                              cgcgATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAagag    >  2:1326036/1‑137 (MQ=255)

                                                                                                                              cgcgATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGtt  >  1:1244986/1‑139 (MQ=255)

                                                                                                                              cgcgATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGtt  >  2:205820/1‑139 (MQ=255)

                                                                                                                                cgATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTa              <  2:1691486/125‑1 (MQ=255)

                                                                                                                                cgATGCCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTa              >  1:1691486/1‑125 (MQ=255)

CTGTAATCCTGAGAGCGCTGGTCAGTGTTTTCGATCAACAAGCCAATGCTGGTGCCGGTAGTAACGCCTTCAAAAACACCGGAGAGAATTTTGACCTGATCCGGCTCGCGGCGCTGGGTGGTATAGCGCGATGTCCCAGGGCGACGACGGTCGAGGTCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTT  >  NC_000913/2447187‑2447451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A