Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F17 I0 R1 95 78.2 1797446 84.3% 1515246 230.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,336,951 A→C 22.1% G27G (GGT→GGG)  adiC ← arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,336,9510AC22.1% 161.2 / 13.0 76G27G (GGT→GGG) adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base A (40/16);  new base C (2/18);  total (42/34)
Fisher's exact test for biased strand distribution p-value = 2.29e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCGACAGTGCGTTCT  >  NC_000913/4336720‑4337186
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            
caTGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGGG‑CACCGATAATCGGCACCAACCATCCATAAATGG‑CAATCCCGCCAGGAGAGGCCAGGGTTGCAGGTAACAG‑AAAAACACCTGAc                                                                                                                                                                                                                                        >  1:348237/1‑238 (MQ=255)
   ggCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTTGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAAACATCCATAAATGG‑CAATCCCGCCAGTAAAGGCCAGGCTTTCAGGGAAAAG‑AAAAACACCTGAccccc                                                                                                                                                                                                                                    >  1:329967/1‑238 (MQ=255)
          ttGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGGAAGAAACAACAGGACTTGGTTCGCGGAACGACCTTTTGGCGGAAACCAACTAAGGCCCCCGTG‑CAGCGATAATCAGCAACAACCATCCATAAAAGA‑CAAACCCCCCCACAGAGGCCCAGCTGGCCAGGAAAAA‑AAAAACCCCAGCCCCCataaaaat                                                                                                                                                                                                                             >  2:654909/1‑235 (MQ=255)
                aTCCAGCAGGCCAGCCAGTAGGGGACGTTGGTTTGATAACCGAGAACCGGGCCAAAGCAGCGGCGGGCGTAAGGGTAAGAACCCCCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGa                                                                                                                                                                                                                       <  1:713290/239‑1 (MQ=255)
                      cagGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCCTAATATTCCCCGccacca                                                                                                                                                                                                                  >  1:204820/1‑238 (MQ=255)
                       agGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCCGTAGAGGCCAGGATTTCAGGTAACAG‑AAAAACACCTGCCCCCATAAAATTCCCCGACACCATc                                                                                                                                                                                                                >  1:404907/1‑239 (MQ=255)
                           cagccagTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCCGTAGAGGCCAGATTTGCCGGGAACAG‑AAAAACACCTGACCCCCTAATATTCCCCGACACCCTCAggg                                                                                                                                                                                                            >  1:382324/1‑239 (MQ=255)
                                   aggggACGTTGGTTTGAAAACCGAGAAGCGGGCAAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACTTTTTGGCGTATCCCATCGAGAGCCCCAGTG‑CACCGATAATCGTCCCCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑Cg                                                                                                                                                                                                      <  1:263308/233‑1 (MQ=255)
                                   agagGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAAAATTCCCCGACACCATCAGGGTGA‑Cgggg                                                                                                                                                                                                   >  1:47268/1‑239 (MQ=255)
                                          gTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGGTAATCGTCCCCAACCATCCATAAAAGG‑CCATCCCCCCAGTAGAGGCCAGGTTTGCAGGGAACAGAAAAAACCCCTAACCCCA‑AATATTTCCCGACACCACCAGGGGGACCGGGGATTaa                                                                                                                                                                                              >  2:400227/1‑238 (MQ=255)
                                            tGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGAAACAG‑AAAAAAACCTGACCCCATAATATTCCCCGAACCCATCAGGGGGA‑CGGGGATTAAGccc                                                                                                                                                                                          >  1:232845/1‑239 (MQ=255)
                                            tGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACGTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTTCAGGGAACAG‑AAAAACACCTGCCCCCCTAAAATTCCCCGAAACAATAAGGGGGA‑AGGGGATAAAAccc                                                                                                                                                                                          >  2:432373/1‑239 (MQ=255)
                                                 tGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAAAGCCCCAGTG‑CACCGGTAATCGTCACCAACCATCCATAAATGG‑CAATCCCCCCACTAGAGGCCAGGTTTGCAGGGAACAA‑AAAAACACCTCACCCCAAAATATTCCCCGCCACCATCAGGGGAA‑CGGGGAGTAAACCCACttt                                                                                                                                                                                     >  2:883174/1‑239 (MQ=255)
                                                        cGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCCTAATATTCCCCGACCCCATCAGGGGGA‑CGGGGATTAAACCCACttttgtggca                                                                                                                                                                              >  2:100297/1‑232 (MQ=255)
                                                        cGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGagca                                                                                                                                                                              >  1:626282/1‑239 (MQ=255)
                                                         gagaAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGGGA‑CGGGGATTAAACCCCCTTTGTGagcct                                                                                                                                                                             >  1:231020/1‑237 (MQ=255)
                                                                      aaGCAGGGGCGGGCGAAAGCGTAAGACCCCCCGGGATTTGGGTCGAGGAACGACATTTTGGCTTATACCATCGAGAGCCCCAGTC‑CACCGATAACCGTCACCACACATCAAAAAAGGC‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGa                                                                                                                                                                <  1:608389/239‑1 (MQ=255)
                                                                          aggggggggcgTAACCGTAAGACCCACAGGGTCGTGGGTGGAGGACCGACATTTTGGCGTATCCCATCGAGGCCCCCAGGG‑CACCGATAATCGTCACCAACCACCAATAAATGC‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACa                                                                                                                                                              <  1:344500/231‑1 (MQ=255)
                                                                                 ggcgTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaaa                                                                                                                                                      >  1:779965/1‑238 (MQ=255)
                                                                                            aagAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCGCCGAAga                                                                                                                                          >  1:249850/1‑238 (MQ=255)
                                                                                            aaccaccacgAGGACTGGGTCCGAGGAACGATTTTTGGGGTTATTCCATCGAGAGCCCCATTA‑CACCGAGAATCGACCCCAAACATCAATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACCCCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAgg                                                                                                                                          <  1:654909/235‑1 (MQ=255)
                                                                                                   accaGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACAAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGCCCAGGTATGCAGTTATGAG‑AAAAACAACTGACCCCCTAATATTTCCCGACACCATCAGGGTGA‑CGGGGATCAAGCCCACTTTCTGCGCATCAGCGTCCGAAGACCTAATAAAACTCCTCCAAAgacaaa                                                                                                                                      >  2:62170/1‑231 (MQ=255)
                                                                                                    ccaGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGGGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTc                                                                                                                                  >  1:404362/1‑239 (MQ=255)
                                                                                                    ccaGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAAGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGCTTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTc                                                                                                                                  >  1:311016/1‑239 (MQ=255)
                                                                                                                  gAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAAtt                                                                                                                     >  1:789492/1‑238 (MQ=255)
                                                                                                                   aGGCACGACTTTTTGGCGTATCCAATCGAGAGCCCC‑GTGCCCCCGATAATCGTCACCAACCAGCCATAAAGGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTa                                                                                                                    <  1:84868/238‑1 (MQ=255)
                                                                                                                   aGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTAc                                                                                                                   >  1:640546/1‑239 (MQ=255)
                                                                                                                   aGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAAACATAATTAAACCCCTGCGAAGGGGAGCTTCGTGACAAAAAATAAc                                                                                                                   >  2:519276/1‑239 (MQ=255)
                                                                                                                     gaccGACTTTTGGGCATATACCATCGAGAGCCCGAGCG‑CACGGATAATCGTCCCCAACCACCCAAAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg                                                                                                                  <  1:519276/235‑1 (MQ=255)
                                                                                                                                     tataCCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCt                                                                                                   >  1:804804/1‑237 (MQ=255)
                                                                                                                                      atcccATCGGGAGCCCGAGTG‑CACCGATAATCGTCCCCAACCATCCATAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCa                                                                                                <  1:883174/236‑1 (MQ=255)
                                                                                                                                           tgggaagaccccATGG‑CACGAATAAACGTCAACAACCATCGATAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                                                                                           <  1:365163/231‑1 (MQ=255)
                                                                                                                                            tCGAGAGCCCCAGTG‑CACCGCTAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTCCGAAGGCGGACTTCGGGACAATAAATTACGGAATAACAAAAGCCTGCATTCgct                                                                                          >  2:165566/1‑237 (MQ=255)
                                                                                                                                            tCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCACACGAAGACATATTTAAACTCCTGCGAAGCCGAGCTCCGCGACAAAAAATTACGTCATATCAAACCCCTTCATTCGac                                                                                          >  2:737706/1‑238 (MQ=255)
                                                                                                                                              gaacacccaAGTG‑CCCCGATAACCGACCCCAACCATCCA‑AAATGGCCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                                                                                           <  1:100297/231‑1 (MQ=255)
                                                                                                                                               agagCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGAGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGGGACAATAAATTACGTCATATAAAACGCCTGCATTCGAAAGt                                                                                       >  2:825083/1‑239 (MQ=255)
                                                                                                                                                 agCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGGGACAATAAATTACGTCATATCATACGCCTGCATTCAATAGTTg                                                                                     >  1:614420/1‑239 (MQ=255)
                                                                                                                                                 agCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGCTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCAAGCTTCGTGACAATAAATTACGTCAAATCATAACCCTGCATTCGAGAGTTg                                                                                     >  1:248091/1‑239 (MQ=255)
                                                                                                                                                   ccccAGTG‑AACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCGGCCAGTAGAGACCAGTGTTGCAGGTAACCG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGAGCGA‑CGGGGATTAAGCCCCCTTGGTGAGCATCAGCATACGAAGACATAATTAAACTCCTGCGAAGGCGAGCTACGAGACAATAAATTACGTCATATCATACCCCCGCATTCGAGAGGTGaa                                                                                   >  2:707050/1‑239 (MQ=255)
                                                                                                                                                        gTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTGGAattatt                                                                              >  1:189721/1‑239 (MQ=255)
                                                                                                                                                            cACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGCCAAATCATACGCCTGCATTCGATAGTTGAATTATtctc                                                                           >  1:285995/1‑239 (MQ=255)
                                                                                                                                                              acaaTACTCCTCACCAA‑CAGCCATAAAAGGCCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGa                                                                         <  1:436378/236‑1 (MQ=255)
                                                                                                                                                               ggatacgcGTCCCCAACCATCAAAAAAGGA‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAc                                                                        <  1:781683/232‑1 (MQ=255)
                                                                                                                                                                 aTAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAACCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTTTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGCGCTTCGTGACAATAAATTACGTCATATCAAACGCCTGCATTTGATAGTTGAATTATTATCAACCg                                                                      >  2:320419/1‑239 (MQ=255)
                                                                                                                                                                 aTAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCCTACGCCTGCATTCGTTAGTTGAATTATTTTCGACCg                                                                      >  1:569294/1‑239 (MQ=255)
                                                                                                                                                                 aTAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCg                                                                      >  1:652141/1‑239 (MQ=255)
                                                                                                                                                                    cactgcatcaaccaacAAAAAAGGG‑CAAGCCCTCCAGTAGAAGCCAAGGTTGCAGGTAACAG‑AAAAACCCCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCggtt                                                                   <  2:874935/231‑3 (MQ=255)
                                                                                                                                                                      cGTCACCAACCACCAAAAAATGG‑CAACCCCGACAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACCCCTGACCCCATAATTATCCCCGGAACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTa                                                                 <  1:737706/239‑1 (MQ=255)
                                                                                                                                                                         cACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATc                                                               >  1:835739/1‑238 (MQ=255)
                                                                                                                                                                         cACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATc                                                               >  1:874202/1‑238 (MQ=255)
                                                                                                                                                                               ccacccatAAAGGG‑CACTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACCCCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATg                                                        <  1:880271/239‑1 (MQ=255)
                                                                                                                                                                                 actcatAAAGGG‑GAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACCCCTGACCCCATAATATTCCCCGACACCATGAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGcc                                                      <  1:62170/236‑1 (MQ=255)
                                                                                                                                                                                  tccatAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCAAACGCCTGCATTCGATAGTTGAATTATTATAAACCGGGTTAACGCGGATGcgg                                                     >  2:778663/1‑237 (MQ=255)
                                                                                                                                                                                   ccatAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACACTAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGt                                                    >  1:467017/1‑239 (MQ=255)
                                                                                                                                                                                    aaaaaaGGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGtt                                                   <  1:129380/236‑1 (MQ=255)
                                                                                                                                                                                      tAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGc                                                                                                     <  1:656882/187‑1 (MQ=255)
                                                                                                                                                                                      tAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGc                                                                                                     >  2:656882/1‑187 (MQ=255)
                                                                                                                                                                                      aaagtGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACCCCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGttat                                                 <  1:432373/235‑1 (MQ=255)
                                                                                                                                                                                       aaagtgcaaaTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACCCCTGGCCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGttat                                                 <  2:789492/231‑1 (MQ=255)
                                                                                                                                                                                       aaaacgcccacccccccAGTTGAGGCCAGGTTTGCAGGTAACAG‑AAAAACCCCTGACCCCATAATATTCCCCGACCCCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGttat                                                 <  2:626282/228‑1 (MQ=255)
                                                                                                                                                                                                 ccccGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACCCCTGACCCCATAATATTCCCCGACACCAGCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGct                                       <  1:400227/238‑1 (MQ=255)
                                                                                                                                                                                                 ccccGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGct                                       <  2:47268/238‑1 (MQ=255)
                                                                                                                                                                                                           tagggCCAGGTTTGGAGGGAACAA‑AAAAAACCCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑GGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACCTAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGtt                                <  2:231020/233‑1 (MQ=255)
                                                                                                                                                                                                           agagGCCAGGTTTGCAGGTAACAG‑AAAAACCCCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTaa                              <  1:825083/238‑1 (MQ=255)
                                                                                                                                                                                                           aaagGCCAGGTTTGAAGGGAACAG‑AAAAACCCCTTACCCCATAATATTCCCCGACACCATCAGGGTGA‑AGGGGATTAAGCCCACTTTGTGAGCATCAGCAACCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGtt                                <  2:640546/234‑1 (MQ=255)
                                                                                                                                                                                                               gCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTa                         >  1:783170/1‑239 (MQ=255)
                                                                                                                                                                                                               gCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTa                         >  1:536211/1‑239 (MQ=255)
                                                                                                                                                                                                                    gTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTAATTAATATAGTTCTTCGTTAAGGTTAAAAAt                    >  2:297319/1‑239 (MQ=255)
                                                                                                                                                                                                                            gTAACAG‑AAAAACCCCTGCCCCCATAATTTTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCGAc            <  2:372009/239‑1 (MQ=255)
                                                                                                                                                                                                                             tAACAG‑AAAAAACCCTGTCCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTCAGCATCAGCATCCGAAGACATAATTAAACTCCTTCGAAGGTGAGATTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCGAc            <  1:803486/238‑1 (MQ=255)
                                                                                                                                                                                                                             tAACAA‑AAAACCCCCTGCCCCCATAATATTCCCCGACCCCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCg              <  2:404362/236‑1 (MQ=255)
                                                                                                                                                                                                                                 cg‑AAAAACACCTGACCCCATAATAGTCCCCGACACCATCGGGGGTA‑CGGGGATTAAGCCCCCTTTGGGAGCATCATAATCATAAGACATAATTAAACACCTGGAAAGGCGAGCTTCTTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCGACAGTGc       <  2:534440/238‑1 (MQ=255)
                                                                                                                                                                                                                                 aG‑AAAACCCCCGGGCCCCAAAATATTCCCCCCCACCAACCGGGTGA‑AGGGGATTAAGCCCACTTTGTGGGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGGCAGTTTCGTGACAATTAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCGACAGTGc       <  2:547296/239‑1 (MQ=255)
                                                                                                                                                                                                                                 aG‑AAAAACCCCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCGACAGTGc       <  1:153396/239‑1 (MQ=255)
                                                                                                                                                                                                                                  aaaaaaaCCCCTGACCCCGTAAGATGTCCCGACGCCATCAGGGGGA‑AGGGGAGTAAGCCCACTTTGTGAGCCACAGCAGCCGAAAACATAATGAAACTCCTGCGAAGGCGAGCTTCGTGGCAATAAATTACGTCATATCATACGCCTGCATTCGATAGGTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCGACAGTGc       <  2:329967/237‑1 (MQ=255)
                                                                                                                                                                                                                                       aaCCCCTGACCCCAGAATTTTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCGACAGTGCGTtct  <  1:274779/239‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            
CATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTG‑CACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAG‑AAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGA‑CGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATATAGCTCTTCGTTAAGGTTAAAAATCAGGCGACAGTGCGTTCT  >  NC_000913/4336720‑4337186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A