Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F17 I0 R1	95	78.2	1797446	84.3%	1515246	230.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,579,656	A→C	24.2%	V62G (GTG→GGG)	symE ←	toxic peptide regulated by antisense sRNA symR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,579,656	0	A	C	24.2%	76.6 / 2.6	48	V62G (GTG→GGG)	symE	toxic peptide regulated by antisense sRNA symR
Reads supporting (aligned to +/- strand): ref base A (33/2); new base C (5/8); total (38/10)
Fisher's exact test for biased strand distribution p-value = 1.21e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTG  >  NC_000913/4579423‑4579888

gTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGTTGGGCGGTGAGGCC‑AATACAGCCTTCCATCACTTTg                                                                                                                                                                                                                                        >  1:610974/1‑239 (MQ=255)

gTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTAAGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGTTGGGCGGGAGGGAC‑AAAAACGCCTTCCACAACtttt                                                                                                                                                                                                                                        >  2:730179/1‑238 (MQ=255)

          aCTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCCGTTCGCTCTCCTCGGCGGAGGGTGGGTGGGCGGGGGGGCC‑AATACAGCCCTCCATCACTTTG‑AAATCTAccc                                                                                                                                                                                                                             >  2:618761/1‑238 (MQ=255)

          aCTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCCATTTG‑ACAttaacc                                                                                                                                                                                                                              >  1:180033/1‑233 (MQ=255)

          aCTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGGTGGGCGGGTAGGAC‑AATAAAGCCTTCCATCACCTTG‑GCATCTAcc                                                                                                                                                                                                                              >  2:69914/1‑238 (MQ=255)

             gAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCCCTTTG‑ACATCTCccgccg                                                                                                                                                                                                                          >  1:374625/1‑239 (MQ=255)

               gggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACAACAACCGCCGt                                                                                                                                                                                                                         >  1:874211/1‑238 (MQ=255)

               gggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACACTTTGCACACCTGGCGTAGCGACTGCCTCAGTTCGCTCCCCTCGGGGGCGGGGGGTTGGGCGGTAAGGCC‑AACACAGCCTTCCACCCACTTG‑AAATCTAcccccgg                                                                                                                                                                                                                         >  2:526232/1‑237 (MQ=255)

                ggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGGGGGGGGGTGGGGGGGAGGGCC‑AATAAAGCCTTCCCCCCCTTTG‑ACATCACCCGCCGTg                                                                                                                                                                                                                        >  2:467750/1‑238 (MQ=255)

                ggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGGC‑AATACAGCCTTCCATCACTTTG‑CCATCTACCGCCGTg                                                                                                                                                                                                                        >  1:212738/1‑238 (MQ=255)

                ggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTg                                                                                                                                                                                                                        >  1:710451/1‑238 (MQ=255)

                ggggAAAGTTTCTACTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTg                                                                                                                                                                                                                        >  1:750574/1‑238 (MQ=255)

                          tCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGCC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCaaa                                                                                                                                                                                                             >  1:570379/1‑239 (MQ=255)

                                  gTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGGGGCAAAACcggcg                                                                                                                                                                                                      >  1:279026/1‑238 (MQ=255)

                                  gTCAGTGGAAGGCTACCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCATTAAACGCCTGCACCTGCTTTTGTTTACGCGCCGCCAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGCGGTTGGGCGGCGCGTCC‑AATACAGCCTTCCTTCACTTTG‑ACATCTACCGCCGTGCCAGTAGCAAAACcggcg                                                                                                                                                                                                      >  1:125741/1‑238 (MQ=255)

                                                cccGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCCCCGTGCCAGTGGCAAAACCGGCGGCTTCCAACCCCTg                                                                                                                                                                                        >  1:460628/1‑238 (MQ=255)

                                                 ccGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCCTCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCATCCCCTGa                                                                                                                                                                                       >  1:731156/1‑238 (MQ=255)

                                                            ccGGGAGTAACTCCGTTTACGCGTCTTTTGTTTTACCGCAAATCACCCAAAAAAACCCCTGCCCCTGTTTTTGTTTCCGCCCCGGCAGTTGGCACACTGGGGGGAGCGACTGAACCAGTCCGCTCTCCCCGGCGGCGGGTGGTGGGGCGGTGAGGAA‑AATCCAGCCTCCCATCACTTTG‑CCATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTg                                                                                                                                                                           <  2:523816/239‑1 (MQ=255)

                                                                 aGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCAAGCCACTGACCTTTCAGGGTGATGGc                                                                                                                                                                      >  1:233168/1‑239 (MQ=255)

                                                                 aGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGCGTGGGCGGGACGGAC‑AATCCAGCCTTCCATCACTTTG‑TCATCTACCGCCGTGCCCGGGGCAAAACCGACGGCTTCCACCCAATGACCCTTCAGGGTAATgac                                                                                                                                                                      >  2:176042/1‑237 (MQ=255)

                                                                          tGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑CCATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCACCCACTTACCTTTCCGGGGGATgcgggggaaaag                                                                                                                                                             >  2:802403/1‑228 (MQ=255)

                                                                                      ttCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCCCTGACCTTTCAGGGGGATGGCGGGAATACG‑GCTGTAATCCgg                                                                                                                                                >  1:445083/1‑239 (MQ=255)

                                                                                              aCCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGGATACG‑GCTGGAATCCGGGTGGCgg                                                                                                                                         >  1:452424/1‑237 (MQ=255)

                                                                                              aCCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGGGAGGGCGGGAAAACGCGCTGAAAACCGGGGAgga                                                                                                                                          >  2:422850/1‑236 (MQ=255)

                                                                                              aCCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCACCCACTGCCCTTTCCGGGGGCGGGCGGGGAAACG‑GCCGTAATCCGGGGGGCGa                                                                                                                                         >  2:880184/1‑238 (MQ=255)

                                                                                              aCCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGATTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACAGCATCAGTTCGCCCTCCTCGGCGTCGGGGGGTTGGGCGGTGAGGGC‑AATGCAGCATTCCATCCCTTTG‑ACATCTACCGCCGTGCCAGTGGCGAAACAGGAGGCTTCCAGCGCATGACCTTTCCGGGGGAGGGCGGGATAACG‑GCTGGAATCCgggggggca                                                                                                                                         >  2:662247/1‑232 (MQ=255)

                                                                                                   cAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGCGGGGAATACG‑GCTGTAAACCCGGGAGCGAccccca                                                                                                                                   >  2:332963/1‑234 (MQ=255)

                                                                                                                    gCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGGTGGCGGGGATAAG‑GCTGTAAACCGGGCAGCCACCCGCCAAAACG‑ACGGGGaaaaa                                                                                                                 >  2:102816/1‑235 (MQ=255)

                                                                                                                        gCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGGAATCCGGGTAGCGACTCGCATAACCA‑ACGGTGACATGACgg                                                                                                             >  1:186802/1‑239 (MQ=255)

                                                                                                                         cACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCAAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGAACCCCTTAACCG‑ACGGTGACATGACGGt                                                                                                            >  1:125141/1‑239 (MQ=255)

                                                                                                                          cccggCTTTTGTTTACGGCCCGAAAGTTTGCCACCCGGGCGAGGGCAAGGCATCAGTTCGCTCTCCGCGGGGGGGGGTGGTTGGGGGGTGAGGAA‑AATACAGCTTTCCATCACTTTG‑ACATTTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGtt                                                                                                           <  1:571765/235‑1 (MQ=255)

                                                                                                                             tGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGCCGGTTAtt                                                                                                        >  1:416819/1‑239 (MQ=255)

                                                                                                                                   tGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCgg                                                                                                  >  1:130179/1‑239 (MQ=255)

                                                                                                                                                      tGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATGCAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGGGATGGCGGGACTACG‑GCTGTAATCCGGGTAACGACTCGCATAAACGAAGGGGGACGAGA‑GGTTTTTTGCCGGGGGAACTTCCGGTTCGAAc                                                                               >  2:336741/1‑239 (MQ=255)

                                                                                                                                                              tGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACTTCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGGGACATGACGGTTATTTGCCGGGGGGACTTCTGCTTCGAACGGtttggc                                                                       >  1:509635/1‑235 (MQ=255)

                                                                                                                                                                      gagcggCATCAGTTCCGTCCCCCGGGCGGGGGGTGGTTGGGCGGTGAGGAA‑AATCCAGCCTCCCATCCCTTGG‑ACATTTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAAt                                                               <  1:102816/234‑1 (MQ=255)

                                                                                                                                                                           gcagtaGTTCGCTCGCGTCGGCGGGGGGTGGTGGGGCGGTGATGAC‑AATCCAGCCTTCCATCCTGTTG‑ACATCTACCGCCGGGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCgtg                                                          <  1:440662/234‑1 (MQ=255)

                                                                                                                                                                                 gTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCACGTGGCAAAACCGGCGGCTTCCAGCCCCTGCCCTTTCAGGGTGATGGCGGGAATACG‑GCCGTAATCCGGGTCGCGACTCCCCTAACCC‑CCCGTGCCATACCAGTTATTTTCCGGGGGGACGTCTGCGTCGCACGGGTGTGCAACAGAAAGCgtgtgcggg                                                    >  2:430592/1‑234 (MQ=255)

                                                                                                                                                                                           ctggggggggggTTGTTGGGGGGTAGGGCA‑AATCCACCCTTCCACCCCTTGG‑ACTGCTCCCGCCGGGGCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTa                                          <  1:430592/230‑1 (MQ=255)

                                                                                                                                                                                                gcgggggTTGTGGGGGGGTG‑GGACAAATCCCGCCTTCCA‑CCCTTTGCACTTCACCCGCCGGGCAAGGGGCAAAACCGGCGGCTGCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATtctc                                     <  2:710451/235‑1 (MQ=255)

                                                                                                                                                                                                 ggcggGTGGTGGGGGGGTGAGGAC‑ATTACAGCCTTCCACCCTTTGG‑ACATCTCCCGCCGTGCCAGTGGCAAAACCGGCGGCTGCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATtctc                                     <  1:764659/238‑1 (MQ=255)

                                                                                                                                                                                                     ggtgttTGGGCGGTGAGGAC‑AATCAACCCTCCCATCCCTTTG‑CCATCTACCGCCGGGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAgg                                 <  1:400553/238‑1 (MQ=255)

                                                                                                                                                                                                     ggtggtTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTTTGCAATAGCATGCGTGTCAGTCATAACTGCTATTTTCCAGGa                                >  1:464561/1‑239 (MQ=255)

                                                                                                                                                                                                               ggggaaGGAA‑AATCCAGCCTCCCATCCTTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAgtgatt                       <  1:513305/233‑1 (MQ=255)

                                                                                                                                                                                                                    aGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCCTAACCG‑ACGGGGACATGCCGGTTATTTGCCGGGGGGACTTCTGCTTTGAAAGGTTGGGCAATAGAATGCGTGTCAGTCATAACAGCCATTCCCCAGGAAAAgggattgggatt                 >  2:695099/1‑239 (MQ=255)

                                                                                                                                                                                                                        aaaaatcAGCCTTCCACCCATTGG‑AACTCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAg               <  1:832193/232‑1 (MQ=255)

                                                                                                                                                                                                                                 ccTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGgt     >  1:487995/1‑239 (MQ=255)

                                                                                                                                                                                                                                    tCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGGGACATGACGGTTTTTTGCCGGGGAGACTTTTGCTTCGAACGGTTGTGCAAAAGAATACGGGTCCGGCATAAATTCCATTATCCAGGAAAAGTGATTGAGAATAACGAAGCgggggga  >  2:294727/1‑235 (MQ=255)

GTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAATAAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTG  >  NC_000913/4579423‑4579888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A