Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,281,475 |
A→T |
M538L (ATG→TTG) |
narG → |
nitrate reductase A subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,281,475 | 0 | A | T | 100.0%
| 97.4
/ NA
| 28 | M538L (ATG→TTG) | narG | nitrate reductase A subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (11/17); total (11/17) |
CGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGA > NC_000913/1281399‑1281550
|
cGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgttgat > 2:229646/1‑81 (MQ=255)
ggCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGAtt < 2:229610/81‑1 (MQ=255)
cGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTAt > 2:229656/1‑81 (MQ=255)
tttCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTAtcg > 2:229663/1‑81 (MQ=255)
tctcGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTAtcgtc > 2:229661/1‑81 (MQ=255)
gCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTg < 2:229611/81‑1 (MQ=255)
cAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGc < 1:229612/81‑1 (MQ=255)
aGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCg < 1:229613/81‑1 (MQ=255)
gCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCgg > 1:229660/1‑81 (MQ=255)
ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAAcc < 1:229614/81‑1 (MQ=255)
ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAAcc < 2:229615/81‑1 (MQ=255)
ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAAc > 2:229655/1‑80 (MQ=255)
gCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGCCGGTGCGGGGCTGAACCACt < 2:229616/81‑1 (MQ=255)
tCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGt < 2:229617/81‑1 (MQ=255)
gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTAt > 1:229649/1‑81 (MQ=255)
ccGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa < 1:229618/81‑1 (MQ=255)
ccGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa > 1:229662/1‑81 (MQ=255)
cGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCAc > 1:229666/1‑81 (MQ=255)
gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc > 1:229665/1‑81 (MQ=255)
tttGCCGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGat < 1:229619/81‑1 (MQ=255)
ccGATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATg < 1:229620/80‑1 (MQ=255)
gATAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc > 2:229672/1‑81 (MQ=255)
tAACGCTGATAAAACGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTa < 2:229621/81‑1 (MQ=255)
aaaCGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa < 1:229623/81‑1 (MQ=255)
aaaCGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa > 1:229669/1‑81 (MQ=255)
aaaCGCACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa < 2:229622/81‑1 (MQ=255)
cACGGTCGTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAAc < 2:229624/81‑1 (MQ=255)
gTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTg < 2:229625/80‑1 (MQ=255)
gTTCGTTGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGa < 1:229626/81‑1 (MQ=255)
|
CGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGA > NC_000913/1281399‑1281550
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
