Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,485,178 |
A→G |
E706E (GAA→GAG) |
hrpA → |
ATP‑dependent RNA helicase HrpA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,485,178 | 0 | A | G | 100.0%
| 65.5
/ NA
| 20 | E706E (GAA→GAG) | hrpA | ATP‑dependent RNA helicase HrpA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/10); total (10/10) |
GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATT > NC_000913/1485100‑1485247
|
ggggCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGcc < 2:261932/81‑1 (MQ=255)
ggggCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGcc < 2:261933/81‑1 (MQ=255)
ggggCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGcc < 2:261934/81‑1 (MQ=255)
tgctgcGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTggg > 2:261957/1‑80 (MQ=255)
tgatgcGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTtgg > 2:261962/4‑80 (MQ=255)
gACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCGCTGGGAACGGGCGCAGGgc > 1:261969/1‑81 (MQ=255)
aaTGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACCGGGAACGGGCGCAGGGCGCGGTGa > 1:261963/1‑81 (MQ=255)
aTGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGAt < 2:261935/81‑1 (MQ=255)
tGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATg > 1:261943/1‑81 (MQ=255)
gggTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATgg > 2:261970/1‑81 (MQ=255)
ggTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATgg < 2:261936/80‑1 (MQ=255)
ggAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAAc > 2:261959/1‑81 (MQ=255)
gAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAAc < 1:261937/80‑1 (MQ=255)
aGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGaaa > 2:261976/1‑80 (MQ=255)
cTCAGCATTTGATTAAACGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTc < 2:261938/80‑1 (MQ=255)
aTTAAACGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGt < 1:261939/81‑1 (MQ=255)
cGCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGcc > 2:261988/1‑80 (MQ=255)
gCACCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGa > 1:261974/1‑81 (MQ=255)
aCCTACAGCGAGCCGCACTGGGAACGGGCGCGGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGAtt < 2:261940/81‑1 (MQ=255)
aCCTACAGCGAGCCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGAtt < 1:261941/81‑1 (MQ=255)
|
GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATT > NC_000913/1485100‑1485247
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
