Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,777,442 |
C→T |
P83S (CCG→TCG) |
ydiO → |
putative acyl‑CoA dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,777,442 | 0 | C | T | 100.0%
| 75.5
/ NA
| 22 | P83S (CCG→TCG) | ydiO | putative acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/9); total (13/9) |
GAAGAATTTGGTGGTATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACG > NC_000913/1777367‑1777512
|
gaagaaTTTGGTGGTATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCa > 2:305427/1‑81 (MQ=255)
aagaaTTTGGTGGTATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCAt < 1:305403/81‑1 (MQ=255)
aTTTGGTGGTATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCAttttt < 1:305404/81‑1 (MQ=255)
tttGGTGGTATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTa < 1:305405/81‑1 (MQ=255)
gtggtATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAAtt < 2:305406/80‑1 (MQ=255)
gtATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCa < 2:305407/81‑1 (MQ=255)
aTCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAAc > 1:305439/1‑81 (MQ=255)
cccTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACgg > 2:305435/1‑81 (MQ=255)
cTGCGGATTACGTCACCCAAATGCTGGCGCTAATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTc < 2:305408/81‑1 (MQ=255)
gATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATg > 1:453478/1‑80 (MQ=255)
aTTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTa > 1:305432/1‑81 (MQ=255)
aTTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTa > 1:305431/1‑81 (MQ=255)
tCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTcaca < 1:305409/81‑1 (MQ=255)
cccAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTa > 1:305436/1‑81 (MQ=255)
aaaTGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATgc > 1:305438/1‑81 (MQ=255)
cgcTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCg < 2:305410/81‑1 (MQ=255)
gcTGATGGAAGTGTCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCgg > 1:305443/1‑81 (MQ=255)
gtgtCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCagag > 1:305437/1‑81 (MQ=255)
tCAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGagca < 2:305411/80‑1 (MQ=255)
cAAAATGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGagcagc > 2:305457/1‑81 (MQ=255)
aaaTGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTa > 2:305441/1‑81 (MQ=255)
aTGCGGTGCTTCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACg > 1:305446/1‑81 (MQ=255)
|
GAAGAATTTGGTGGTATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACG > NC_000913/1777367‑1777512
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
