Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,887,620 |
C→T |
E124E (GAG→GAA) |
rnd ← |
RNase D |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,887,620 | 0 | C | T | 100.0%
| 74.5
/ NA
| 22 | E124E (GAG→GAA) | rnd | RNase D |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/11); total (11/11) |
CTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGG > NC_000913/1887548‑1887697
|
cTGACGTTAGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTccac < 1:322285/81‑1 (MQ=255)
gACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTccacca > 2:322340/1‑81 (MQ=255)
ttCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGa < 2:322286/81‑1 (MQ=255)
ttCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGa < 2:322287/81‑1 (MQ=255)
ggTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAg < 2:322288/80‑1 (MQ=255)
gCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAAc > 2:322327/1‑81 (MQ=255)
gCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTCCCACCATGGAAGCGAAAc > 2:322330/1‑81 (MQ=255)
ggTCTGGCCATCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAAccc > 1:322332/1‑81 (MQ=255)
ggTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAAccc < 2:322289/81‑1 (MQ=255)
cagccagTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACAt < 2:322290/81‑1 (MQ=255)
gccagTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCg > 1:322343/1‑81 (MQ=255)
ccagTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCgg < 1:322291/81‑1 (MQ=255)
ggTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTcc > 1:322322/1‑81 (MQ=255)
ggTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTc < 1:322292/80‑1 (MQ=255)
tGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGc < 2:322293/81‑1 (MQ=255)
ttGTCCAGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAg > 1:322325/1‑80 (MQ=255)
aGCGTAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGc < 2:322294/81‑1 (MQ=255)
gTAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgtg > 1:322350/1‑81 (MQ=255)
tAACGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgtgt < 1:322295/81‑1 (MQ=255)
aaCGCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgtgt > 2:322334/1‑80 (MQ=255)
gCCGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaat > 2:322312/1‑81 (MQ=255)
ccGGAATATTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaat > 2:322344/1‑80 (MQ=255)
atatTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAggg < 2:322296/81‑1 (MQ=255)
|
CTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGG > NC_000913/1887548‑1887697
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
