Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,936,676 |
T→C |
S9P (TCT→CCT) |
yebK → |
DNA‑binding transcriptional repressor YebK |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,936,676 | 0 | T | C | 100.0%
| 64.2
/ NA
| 19 | S9P (TCT→CCT) | yebK | DNA‑binding transcriptional repressor YebK |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/6); total (13/6) |
TTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCC > NC_000913/1936600‑1936751
|
ttCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGcct > 2:330528/1‑79 (MQ=255)
tctttcAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGa < 1:330506/80‑1 (MQ=255)
ctttcAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGaa > 2:330529/1‑80 (MQ=255)
tcAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAAc < 1:330507/78‑1 (MQ=255)
aGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAACAttt > 1:330536/1‑80 (MQ=255)
gTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAACAttt > 2:330526/1‑79 (MQ=255)
aaaTCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAACATTTGAGCAAAt < 1:330508/81‑1 (MQ=255)
tACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCa > 1:330530/1‑80 (MQ=255)
gTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAATATTTGAGCAAATCAGAGCGCAAAGTTGcc > 2:330527/1‑79 (MQ=255)
tctTTACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGt > 1:330532/1‑81 (MQ=255)
tttACATCATGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCa > 1:330533/1‑81 (MQ=255)
atGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCt > 1:330540/1‑81 (MQ=255)
atGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCt < 1:330509/81‑1 (MQ=255)
tGAATATGCTGGAAAAAATCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCtt < 1:330510/81‑1 (MQ=255)
aaTATGCTGGAAAAAATCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCg > 2:330537/1‑81 (MQ=255)
ggAAAAAATCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATaa > 1:330538/1‑81 (MQ=255)
aaaaaaTCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAAcg > 1:330542/1‑81 (MQ=255)
aaaTCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGa > 1:330535/1‑81 (MQ=255)
tCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATcc < 1:330511/81‑1 (MQ=255)
tCCAGCCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATc > 2:330534/1‑80 (MQ=255)
|
TTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCC > NC_000913/1936600‑1936751
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
