Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,953,658 |
G→T |
A73S (GCA→TCA) |
cmoB → |
tRNA U34 carboxymethyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,953,658 | 0 | G | T | 100.0%
| 102.6
/ NA
| 30 | A73S (GCA→TCA) | cmoB | tRNA U34 carboxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/13); total (17/13) |
TTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGG > NC_000913/1953583‑1953734
|
tttCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAggg < 1:333468/81‑1 (MQ=255)
ccTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCaa < 2:333469/78‑1 (MQ=255)
cTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAAtt > 2:333510/1‑80 (MQ=255)
cTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAAtt > 2:333509/1‑80 (MQ=255)
tGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTa > 1:333501/1‑80 (MQ=255)
aaTTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACg > 2:333506/1‑81 (MQ=255)
aaCCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCAtt < 1:333471/80‑1 (MQ=255)
aaCCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAAGGCAAATTAAACGCAtt < 2:333470/80‑1 (MQ=255)
cGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGaaa < 1:333472/81‑1 (MQ=255)
cGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCt > 1:333514/1‑80 (MQ=255)
gTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTg < 1:333473/80‑1 (MQ=255)
ttGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATgc > 1:333518/1‑81 (MQ=255)
tGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATgcg > 1:333507/1‑81 (MQ=255)
gATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATgc < 2:333474/79‑1 (MQ=255)
ttGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCt < 2:333475/80‑1 (MQ=255)
ttGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCt < 1:333476/80‑1 (MQ=255)
tGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGa > 2:333521/1‑81 (MQ=255)
aGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCg > 1:333504/1‑81 (MQ=255)
gCGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCg < 2:333477/80‑1 (MQ=255)
cGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTg > 2:333512/1‑81 (MQ=255)
cGTTACGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTg > 2:333517/1‑81 (MQ=255)
cGGCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGgcgc < 2:333478/80‑1 (MQ=255)
ggCAGAAAGCGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCa > 1:333508/1‑80 (MQ=255)
cGAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGtt < 1:333479/81‑1 (MQ=255)
gAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTtc > 1:333522/1‑81 (MQ=255)
gAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTtc > 1:333516/1‑81 (MQ=255)
gAAGAGCCACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTtc > 2:333528/1‑81 (MQ=255)
cACTGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGt < 2:333480/81‑1 (MQ=255)
tGAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATg > 1:333526/1‑81 (MQ=255)
gAGCTCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATgg > 2:333513/1‑81 (MQ=255)
|
TTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCATAGCGTTACGGCAGAAAGCGAAGAGCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGG > NC_000913/1953583‑1953734
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
