Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,992,311 |
C→T |
L169L (TTG→TTA) |
pgsA ← |
phosphatidylglycerophosphate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,992,311 | 0 | C | T | 100.0%
| 73.5
/ NA
| 22 | L169L (TTG→TTA) | pgsA | phosphatidylglycerophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/7); total (15/7) |
TTTGATCGTTTGCTGAAAATTACGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGCAACATTGACCACAGAGTCAGTACCGCAGCCACAAAGAAAAGTGCAATACCGGCGTACTCAACCCAAATGTTCG > NC_000913/1992236‑1992384
|
tttGATCGTTTGCTGAAAATTACGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACAt > 2:339858/1‑81 (MQ=255)
tttGATCGTTTGCTGAAAATTACGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACAt > 1:339852/1‑81 (MQ=255)
tGATCGTTTGCTGAAAATTACGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACAtt < 2:339825/80‑1 (MQ=255)
cGTTTGCTGAAAATTACGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTATAACATTGACca > 2:339850/1‑81 (MQ=255)
gTTTGCTGAAAATTACGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATAATGTAACATTGACca > 1:339848/1‑80 (MQ=255)
aaaaTTACGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAg > 1:339849/1‑81 (MQ=255)
cGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCg > 2:339860/1‑79 (MQ=255)
cGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCa < 1:339826/81‑1 (MQ=255)
gCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAg > 2:339853/1‑81 (MQ=255)
cGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGc > 2:339855/1‑80 (MQ=255)
gAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCca < 1:339827/81‑1 (MQ=255)
aaCGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCcaca < 1:405379/81‑1 (MQ=255)
cGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCcaca > 2:339845/1‑79 (MQ=255)
cGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCcaca > 1:339863/1‑79 (MQ=255)
cGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCCACaaa < 2:339828/81‑1 (MQ=255)
aCTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCCACAAAGaa > 2:339864/1‑79 (MQ=255)
aaaTCTGCACGCGCAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCCACAAAGAAAAGTGCAATACCg > 2:339857/1‑81 (MQ=255)
gcgcAGCGCTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCCACAAAGAAAAGTGCAATACCGGCGTACTCaa > 1:339870/1‑81 (MQ=255)
gcgcTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCCACAAAGAAAAGTGCAATACCGGCGTACTCAACCCaa < 1:339829/81‑1 (MQ=255)
gcTCAAATATTGTAACATTGACCACAGAGTCAGTACCGCAGCCACAAAGAAAAGTGCAATACCGGCGTACTCAACCCAAAt > 1:339859/1‑81 (MQ=255)
aaaTATTGTAACATTGACCACAGAGTCAGTACCGCAGCCACAAAGAAAAGTGCAATACCGGCGTACTCAACCCAAATGTTc > 2:339872/1‑81 (MQ=255)
aaTATTGTAACATTGACCACAGAGTCAGTACCGCAGCCACAAAGAAAAGTGCAATACCGGCGTACTCAACCCAAATGTTCg < 1:339830/81‑1 (MQ=255)
|
TTTGATCGTTTGCTGAAAATTACGCCGAAACGATCACTGATCAAGCAAATCTGCACGCGCAGCGCTCAAATATTGCAACATTGACCACAGAGTCAGTACCGCAGCCACAAAGAAAAGTGCAATACCGGCGTACTCAACCCAAATGTTCG > NC_000913/1992236‑1992384
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
