Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,165,858 |
T→A |
V64D (GTC→GAC) |
yegQ → |
putative peptidase YegQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,165,858 | 0 | T | A | 100.0%
| 101.7
/ NA
| 30 | V64D (GTC→GAC) | yegQ | putative peptidase YegQ |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (15/15); total (15/15) |
CGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGC > NC_000913/2165782‑2165935
|
cacaacatCGAATTCAACCACGAAAATCATCAGCTCGGGATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACgg < 2:368459/78‑2 (MQ=255)
caacaaCGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGt > 2:368495/1‑81 (MQ=255)
aacGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCaa > 2:368507/1‑80 (MQ=255)
aacGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCaa > 1:368512/1‑80 (MQ=255)
aaTTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTg < 1:368460/81‑1 (MQ=255)
ttCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCa < 1:368461/81‑1 (MQ=255)
cAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCAc < 1:368462/80‑1 (MQ=255)
aaCCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCg > 1:368500/1‑81 (MQ=255)
ccACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGca < 1:368463/81‑1 (MQ=255)
cGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACaa < 2:368464/81‑1 (MQ=255)
gAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAAc > 2:368503/1‑81 (MQ=255)
gAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAAc > 1:368509/1‑81 (MQ=255)
cAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCaa < 1:368466/77‑1 (MQ=255)
cAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGc < 1:368465/79‑1 (MQ=255)
cAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCt < 2:368467/80‑1 (MQ=255)
cGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCTACATTGCACCGCACAACGCCAAGCTGAAAAc > 2:368532/1‑81 (MQ=255)
aTGAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATcc < 2:368468/81‑1 (MQ=255)
gAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGt > 1:368510/1‑81 (MQ=255)
gAAGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGt > 2:368516/1‑81 (MQ=255)
aGCCCACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGa > 2:368525/1‑81 (MQ=255)
cccACGCGCTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGAcc < 1:368469/81‑1 (MQ=255)
gcgcTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGaaa < 1:368470/81‑1 (MQ=255)
cTGGGGAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCg < 2:368471/81‑1 (MQ=255)
ggggAAAAAGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCggt > 1:368529/1‑81 (MQ=255)
aaaaaGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGgtggtg > 2:368508/1‑81 (MQ=255)
aaGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGa > 1:368519/1‑80 (MQ=255)
aaGTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGa > 2:368526/1‑80 (MQ=255)
gTTTTATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAAt < 1:368472/81‑1 (MQ=255)
ttATGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATgg < 1:368473/80‑1 (MQ=255)
aTGACGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGc > 1:368521/1‑81 (MQ=255)
|
CGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACCGGTGGTGGAAATGGGGC > NC_000913/2165782‑2165935
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
