Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,316,520 |
A→T |
D115V (GAT→GTT) |
rcsB → |
DNA‑binding transcriptional activator RcsB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,316,520 | 0 | A | T | 100.0%
| 70.7
/ NA
| 22 | D115V (GAT→GTT) | rcsB | DNA‑binding transcriptional activator RcsB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (9/13); total (9/13) |
AACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAA > NC_000913/2316447‑2316594
|
aacCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGcc > 2:394512/1‑80 (MQ=255)
cccGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGaa < 1:568610/81‑1 (MQ=255)
ggCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGc < 2:394490/81‑1 (MQ=255)
cGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGctc > 2:394505/1‑81 (MQ=255)
gATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCT‑AAACAAGGTGCACCGACCGTTCTGCCGAAAGCtctc < 2:394491/81‑1 (MQ=255)
aTTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCtctc < 2:394492/81‑1 (MQ=255)
aGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCTCTcgcc < 1:394493/78‑1 (MQ=255)
tGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCt < 1:394494/81‑1 (MQ=255)
tGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAg > 1:394515/1‑81 (MQ=255)
aTCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAggg > 2:394521/1‑80 (MQ=255)
cTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAggg > 1:394527/1‑78 (MQ=255)
tCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAAttt < 2:394495/80‑1 (MQ=255)
ggATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCgg < 2:394497/81‑1 (MQ=255)
ggATCGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCgg < 1:394496/81‑1 (MQ=255)
cGTGCTGAAACAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAg > 1:394522/1‑81 (MQ=255)
aaCAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTtctc > 1:394517/1‑81 (MQ=255)
aaCAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTtctc < 2:394498/81‑1 (MQ=255)
aaCAAGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTtctc < 1:394499/81‑1 (MQ=255)
cAAGGTGCACCGAACGTTCTGAAGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGc < 1:394500/81‑1 (MQ=255)
aaGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGcc > 1:394519/1‑81 (MQ=255)
aGGTGCACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCt > 1:394514/1‑81 (MQ=255)
cACCGACCGTTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTgg < 1:394501/81‑1 (MQ=255)
gaccgtTCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGaaa > 1:394530/1‑80 (MQ=255)
|
AACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAA > NC_000913/2316447‑2316594
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
