Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,378,194 |
C→A |
A356S (GCA→TCA) |
menD ← |
2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,378,194 | 0 | C | A | 100.0%
| 134.9
/ NA
| 38 | A356S (GCA→TCA) | menD | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (23/15); total (23/15) |
CAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTC > NC_000913/2378120‑2378266
|
cAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTc > 2:405710/1‑81 (MQ=255)
aGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTcc < 2:405668/81‑1 (MQ=255)
aTAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGc < 1:405669/81‑1 (MQ=255)
tAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGcc < 1:405670/81‑1 (MQ=255)
tAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGcc < 2:405671/81‑1 (MQ=255)
gTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAg < 2:405672/81‑1 (MQ=255)
cGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGgc > 2:405739/1‑81 (MQ=255)
atgcgatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCggg < 1:405673/81‑1 (MQ=255)
cgatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGAtt > 2:405750/1‑81 (MQ=255)
cgatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGAtt < 1:405674/81‑1 (MQ=255)
gatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGAttt > 1:405740/1‑81 (MQ=255)
atgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGAttt > 1:405711/1‑80 (MQ=255)
tgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTc > 2:405738/1‑80 (MQ=255)
cAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCa > 1:405721/1‑81 (MQ=255)
aGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCAc > 1:405709/1‑81 (MQ=255)
gcgcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAgg > 2:405730/1‑81 (MQ=255)
cgcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAgg < 1:405675/80‑1 (MQ=255)
gcTTCGCCAAACGCATCACGGCGTGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGc > 2:405729/1‑81 (MQ=255)
gcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGc > 1:405715/1‑81 (MQ=255)
tCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTgg > 2:405727/1‑81 (MQ=255)
gCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGc < 2:405676/80‑1 (MQ=255)
aaaCGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGttt > 1:405714/1‑81 (MQ=255)
aaaCGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGttt > 1:405722/1‑81 (MQ=255)
aCGCATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGttttt > 1:405734/1‑81 (MQ=255)
cATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTg < 2:405677/81‑1 (MQ=255)
cATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTg < 1:405678/81‑1 (MQ=255)
cATCACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTg > 2:405716/1‑81 (MQ=255)
cACGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCg > 2:405717/1‑81 (MQ=255)
aCGGCGGGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCg > 1:405708/1‑80 (MQ=255)
cggcggGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGa > 2:405724/1‑81 (MQ=255)
gcggGCAATAACCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATg < 1:405679/81‑1 (MQ=255)
aaCCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccag > 1:405741/1‑81 (MQ=255)
aCCGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagc < 1:405680/81‑1 (MQ=255)
cGCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagc > 2:405731/1‑79 (MQ=255)
gCCTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagccag < 1:405681/81‑1 (MQ=255)
ccTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGt > 2:405735/1‑81 (MQ=255)
ccTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGt < 1:405682/81‑1 (MQ=255)
cTGCATTGACTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTc > 1:405732/1‑81 (MQ=255)
|
CAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTC > NC_000913/2378120‑2378266
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
