Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,417,900 |
C→T |
Q274* (CAG→TAG) |
yfcC → |
putative transporter YfcC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,417,900 | 0 | C | T | 100.0%
| 112.2
/ NA
| 32 | Q274* (CAG→TAG) | yfcC | putative transporter YfcC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/15); total (17/15) |
GTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGG > NC_000913/2417825‑2417977
|
gtgtACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGc < 1:412783/80‑1 (MQ=255)
tACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGAt < 1:412784/81‑1 (MQ=255)
tACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGAt < 1:412785/81‑1 (MQ=255)
tACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGAt > 1:412834/1‑81 (MQ=255)
ccTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGtt > 1:412822/1‑80 (MQ=255)
cGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGaacaa > 2:412816/1‑81 (MQ=255)
gAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGaacaac > 1:412831/1‑81 (MQ=255)
tGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTc < 2:412786/81‑1 (MQ=255)
gAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTcc > 2:412819/1‑81 (MQ=255)
aaaaaGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTcc < 2:412788/80‑1 (MQ=255)
aaaaaGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCg < 2:412787/81‑1 (MQ=255)
aaaGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGtt < 1:412789/81‑1 (MQ=255)
aaGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGttt < 1:412790/81‑1 (MQ=255)
aGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTa > 1:412830/1‑81 (MQ=255)
ccTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCttt > 1:412821/1‑81 (MQ=255)
tcttctGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTgg > 2:412841/1‑81 (MQ=255)
ctGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGAc > 2:412832/1‑81 (MQ=255)
tCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGAct < 1:412791/79‑1 (MQ=255)
aCGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGActggct > 1:412826/1‑81 (MQ=255)
aCGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGActggct > 2:412827/1‑81 (MQ=255)
aCGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGActggc < 1:412792/80‑1 (MQ=255)
gCATGAGTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTatt > 1:412837/1‑81 (MQ=255)
gTCCGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGt > 1:412839/1‑81 (MQ=255)
ccGACCGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTcc > 2:412833/1‑81 (MQ=255)
cGCTTCTTTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGAcc < 2:412793/81‑1 (MQ=255)
cttTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGAccgcc < 2:412794/79‑1 (MQ=255)
cttTCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGAccgcc < 1:412795/79‑1 (MQ=255)
tCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGt < 1:412796/78‑1 (MQ=255)
tCGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAAt > 2:412836/1‑81 (MQ=255)
cGTGAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATg > 2:412857/1‑81 (MQ=255)
gAAAAGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCCGGTATTGATTGTCCTGACCGCCGTAATGGTc > 2:412829/1‑81 (MQ=255)
aaGTAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTgg < 2:412797/81‑1 (MQ=255)
|
GTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGG > NC_000913/2417825‑2417977
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
