Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,775,431 |
G→A |
intergenic (+410/‑114) |
yfjW → / → ypjI |
CP4‑57 prophage; uncharacterized protein YfjW/putative UPF0401 protein YpjI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,775,431 | 0 | G | A | 100.0%
| 60.4
/ NA
| 18 | intergenic (+410/‑114) | yfjW/ypjI | CP4‑57 prophage; uncharacterized protein YfjW/putative UPF0401 protein YpjI |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (6/12); total (6/12) |
GCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAG > NC_000913/2775362‑2775505
|
gCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGc > 1:477197/1‑80 (MQ=255)
cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAat < 1:477176/81‑1 (MQ=255)
cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAat < 2:477175/81‑1 (MQ=255)
ccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAata < 1:477177/81‑1 (MQ=255)
tACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAatat > 1:477196/1‑80 (MQ=255)
aTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCa < 1:477178/81‑1 (MQ=255)
tGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCAt < 2:477179/81‑1 (MQ=255)
cATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGa < 2:477180/81‑1 (MQ=255)
ggCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCt < 1:477181/81‑1 (MQ=255)
gTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACa < 1:477182/81‑1 (MQ=255)
cTGGCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACaaa > 2:477200/1‑81 (MQ=255)
gCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAAc < 1:477183/79‑1 (MQ=255)
gCACTCCAACTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACa > 2:477198/1‑80 (MQ=255)
aaCTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGAt < 1:477184/81‑1 (MQ=255)
aCTTCCGCTTTTGGCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGAtt < 2:477185/81‑1 (MQ=255)
ttttGGCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACgg > 2:477202/1‑78 (MQ=255)
gCACAGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAAtc < 2:477186/80‑1 (MQ=255)
caGAACAGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAg > 2:477203/1‑81 (MQ=255)
|
GCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAG > NC_000913/2775362‑2775505
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
