Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,080,037 |
C→A |
L533L (CTG→CTT) |
tktA ← |
transketolase 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,080,037 | 0 | C | A | 100.0%
| 176.4
/ NA
| 50 | L533L (CTG→CTT) | tktA | transketolase 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (28/22); total (28/22) |
TGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACG > NC_000913/3079959‑3080111
|
tGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAg > 2:534937/1‑81 (MQ=255)
aCCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCAACGCGCGCGATGTTTGCAAGTTg > 1:534936/1‑81 (MQ=255)
ccGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGc < 1:534884/81‑1 (MQ=255)
cGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGct < 2:534886/81‑1 (MQ=255)
cGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGct < 2:534885/81‑1 (MQ=255)
ggTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGctc < 1:534887/81‑1 (MQ=255)
gTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGctct > 2:534945/1‑81 (MQ=255)
gCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTc < 1:534888/80‑1 (MQ=255)
aaaTCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAgttcgtt > 2:534941/1‑81 (MQ=255)
aTCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTcc < 1:534889/81‑1 (MQ=255)
cAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCctg > 2:534935/1‑81 (MQ=255)
cAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCctg > 1:534975/1‑81 (MQ=255)
aGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCctgc > 1:534909/1‑81 (MQ=255)
tCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCtgctgc > 1:534951/1‑81 (MQ=255)
ccGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTgcg > 1:534954/1‑81 (MQ=255)
cGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTgcg < 1:534890/80‑1 (MQ=255)
gCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTgcgc > 2:689673/1‑79 (MQ=255)
gCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCa < 1:534891/81‑1 (MQ=255)
cTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAg > 1:534938/1‑81 (MQ=255)
cTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAg < 2:534892/81‑1 (MQ=255)
cTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAg > 1:534969/1‑81 (MQ=255)
gACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGt > 1:534942/1‑81 (MQ=255)
aCCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAgg < 1:534893/79‑1 (MQ=255)
cGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCt > 2:534960/1‑81 (MQ=255)
gcgcAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGa < 2:534894/81‑1 (MQ=255)
gcAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACg > 2:534964/1‑81 (MQ=255)
gcAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACg < 1:534895/81‑1 (MQ=255)
cAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACgg < 1:534896/81‑1 (MQ=255)
aGTCTTTCAGCACATAACCACCGCGCGCGATATTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACggg < 2:534897/81‑1 (MQ=255)
tCTTTCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGgag < 1:534898/81‑1 (MQ=255)
ttCAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGagag > 2:534958/1‑80 (MQ=255)
cAGCACATAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGAt > 1:534962/1‑81 (MQ=255)
caTAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTg > 2:534952/1‑81 (MQ=255)
aTAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGc > 1:534965/1‑81 (MQ=255)
tAACCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCg < 2:534899/81‑1 (MQ=255)
aaCCACCGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCgg > 2:534949/1‑81 (MQ=255)
aaCCACCGCGCGCGATGTTTGCAAGTTGCTCCTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCgc > 1:534959/1‑80 (MQ=255)
accaccGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGt > 2:534950/1‑81 (MQ=255)
accaccGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGt < 2:534900/81‑1 (MQ=255)
ccaccGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTc < 1:534901/81‑1 (MQ=255)
ccaccGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTc < 1:534902/81‑1 (MQ=255)
accGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCgg < 1:534903/81‑1 (MQ=255)
accGCGCGCGATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCgg > 2:534971/1‑81 (MQ=255)
cgcgcgcgATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGc > 2:534970/1‑81 (MQ=255)
gcgcgATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGt < 1:534904/81‑1 (MQ=255)
gcgcgATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGt > 2:534955/1‑81 (MQ=255)
cgATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCt > 1:534948/1‑81 (MQ=255)
gATGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCt > 1:534967/1‑80 (MQ=255)
aTGTTTGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGa > 2:534956/1‑81 (MQ=255)
tttGCAAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACg < 1:534905/80‑1 (MQ=255)
|
TGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACG > NC_000913/3079959‑3080111
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
