Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,081,649 |
C→T |
intergenic (‑14/‑264) |
tktA ← / → loiP |
transketolase 1/metalloprotease LoiP |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,081,649 | 0 | C | T | 100.0%
| 119.7
/ NA
| 34 | intergenic (‑14/‑264) | tktA/loiP | transketolase 1/metalloprotease LoiP |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/21); total (13/21) |
TTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAA > NC_000913/3081573‑3081722
|
tttCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGgat > 2:535330/1‑81 (MQ=255)
tGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGgatgat > 2:535361/1‑80 (MQ=255)
tACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGatgatg < 2:535297/79‑1 (MQ=255)
aCTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAg > 2:535346/1‑81 (MQ=255)
tGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAggg > 2:535343/1‑81 (MQ=255)
gTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCAc > 1:535353/1‑81 (MQ=255)
gTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCAc > 1:535344/1‑81 (MQ=255)
cATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGcc < 1:535299/81‑1 (MQ=255)
cATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGc < 1:535298/80‑1 (MQ=255)
cTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTa < 1:535300/81‑1 (MQ=255)
tAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTgacgac < 1:535301/81‑1 (MQ=255)
aGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTgacga > 2:535356/1‑79 (MQ=255)
aTTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAgcg < 1:535302/81‑1 (MQ=255)
ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAgcgc < 2:535303/81‑1 (MQ=255)
ggCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGcgcg < 1:535304/80‑1 (MQ=255)
aaGCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGtttt < 2:535305/81‑1 (MQ=255)
gCTCTTTACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTgg < 1:535306/81‑1 (MQ=255)
tACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCt < 1:535308/78‑1 (MQ=255)
tACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTa < 1:535307/79‑1 (MQ=255)
tACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTAc < 1:535309/80‑1 (MQ=255)
tACGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACg > 2:535349/1‑81 (MQ=255)
aCGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACg < 2:535310/80‑1 (MQ=255)
aCGTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGc > 2:535366/1‑81 (MQ=255)
gTGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCg < 2:535311/81‑1 (MQ=255)
tGAGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCgg < 2:535312/81‑1 (MQ=255)
aGGACATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGa < 2:535313/80‑1 (MQ=255)
aCATTTTGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAAt < 1:535314/81‑1 (MQ=255)
ttttGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTg > 1:535357/1‑81 (MQ=255)
ttttGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTg < 1:535315/81‑1 (MQ=255)
ttGACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGc > 2:535358/1‑80 (MQ=255)
gACTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGTGCTACGCCGGAAAATTTGCcaa > 1:535347/1‑81 (MQ=255)
aCTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCcaac < 2:535316/81‑1 (MQ=255)
aCTCCAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCcaa > 1:535345/1‑80 (MQ=255)
cAGATTGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCcaacaa < 2:535317/79‑1 (MQ=255)
|
TTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAA > NC_000913/3081573‑3081722
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
