Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,091,502 |
T→C |
R123R (CGT→CGC) |
rsmE → |
16S rRNA m(3)U1498 methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,091,502 | 0 | T | C | 100.0%
| 117.5
/ NA
| 34 | R123R (CGT→CGC) | rsmE | 16S rRNA m(3)U1498 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/17); total (17/17) |
AAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCC > NC_000913/3091425‑3091576
|
aaaTCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTg < 2:537311/81‑1 (MQ=255)
atcgaACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAAc > 2:537351/1‑78 (MQ=255)
atcgaACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAAc > 1:537367/1‑78 (MQ=255)
gaACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACaagaa < 2:537312/80‑1 (MQ=255)
aCTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCt > 1:537346/1‑81 (MQ=255)
ggTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTc < 2:537313/79‑1 (MQ=255)
gtgtAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTcagc < 2:537314/81‑1 (MQ=255)
aTTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCagaa < 1:537315/80‑1 (MQ=255)
ttACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCagaaga < 2:537316/81‑1 (MQ=255)
tACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGAt > 2:537361/1‑81 (MQ=255)
cGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGAtt < 1:537317/80‑1 (MQ=255)
ccACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCa < 1:537318/81‑1 (MQ=255)
cACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCaa > 1:537355/1‑81 (MQ=255)
aCTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAAt < 2:537319/81‑1 (MQ=255)
tCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATtgctgc < 1:537320/80‑1 (MQ=255)
gAGCGCTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCtgt > 2:537348/1‑81 (MQ=255)
gcgcTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGa < 1:537321/81‑1 (MQ=255)
gcTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAg > 1:537358/1‑80 (MQ=255)
gcTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAg > 1:537369/1‑80 (MQ=255)
cTGCGGCGTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCa < 2:537322/81‑1 (MQ=255)
gcggcgTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAgt > 2:537352/1‑81 (MQ=255)
cggcgTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAgtg > 1:537376/1‑81 (MQ=255)
gcgTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGtgtg < 1:537323/81‑1 (MQ=255)
gTTAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGt > 1:537349/1‑81 (MQ=255)
ttAAACTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTc > 2:537356/1‑81 (MQ=255)
aaaCTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCg > 2:537354/1‑80 (MQ=255)
aCTGGATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTaa > 1:537366/1‑81 (MQ=255)
gATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAAcc < 2:537326/79‑1 (MQ=255)
gATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAAcc < 1:537324/79‑1 (MQ=255)
gATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCgg > 1:537359/1‑81 (MQ=255)
gATAGTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCg < 2:537325/80‑1 (MQ=255)
gTGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGc > 2:537350/1‑81 (MQ=255)
tGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGcc < 2:537327/81‑1 (MQ=255)
tGAACGCCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGcc > 2:537364/1‑81 (MQ=255)
|
AAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCC > NC_000913/3091425‑3091576
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
