Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,103,953 |
G→A |
C314Y (TGC→TAC) |
mutY → |
adenine DNA glycosylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,103,953 | 0 | G | A | 100.0%
| 106.6
/ NA
| 31 | C314Y (TGC→TAC) | mutY | adenine DNA glycosylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/17); total (14/17) |
CCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGG > NC_000913/3103875‑3104024
|
ccGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACa > 2:539863/1‑81 (MQ=255)
cgcgTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACAt < 1:539812/81‑1 (MQ=255)
tttCGGCATACCTTCAGCTATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGAt > 2:539867/1‑81 (MQ=255)
ttCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGAt > 1:539864/1‑80 (MQ=255)
aCCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAAt > 1:539870/1‑81 (MQ=255)
ccTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAAt < 1:539813/80‑1 (MQ=255)
ccTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAAt < 2:539814/80‑1 (MQ=255)
ccTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATg < 2:539815/81‑1 (MQ=255)
cTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATgc > 1:539874/1‑81 (MQ=255)
ttCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGta > 2:539866/1‑80 (MQ=255)
ttCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGta > 2:539876/1‑80 (MQ=255)
tCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGtat < 2:539816/80‑1 (MQ=255)
gATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGTTGAAGGCAATGCGCTCTGGTATAACTTAgcg > 2:539868/1‑81 (MQ=255)
gATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAgc < 1:539818/80‑1 (MQ=255)
gATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGAATGAAGGCAATGCGCTCTGGTATAACTTGgcg < 2:539817/81‑1 (MQ=255)
gtgCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAccg > 1:539877/1‑81 (MQ=255)
gtgCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAcc < 1:539819/80‑1 (MQ=255)
tgCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAccgc < 1:539820/81‑1 (MQ=255)
tgCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAccg < 1:539821/80‑1 (MQ=255)
cTATGTGGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGt > 1:539878/1‑81 (MQ=255)
tgtgGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAg < 2:539822/81‑1 (MQ=255)
tgtgGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAg < 1:539823/81‑1 (MQ=255)
tgGCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGtt < 2:539824/81‑1 (MQ=255)
gCTTCCCGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTgg > 2:539881/1‑81 (MQ=255)
cGTGTCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGc < 2:539825/81‑1 (MQ=255)
gtgtCGTCATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCg > 1:539880/1‑81 (MQ=255)
gtcgtcATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGc > 2:539892/1‑81 (MQ=255)
gtcATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTcc > 2:539884/1‑81 (MQ=255)
tcATTCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTccc < 2:539826/81‑1 (MQ=255)
ttCACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTg < 1:539827/81‑1 (MQ=255)
cACCGGCTACATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTgg < 1:539828/80‑1 (MQ=255)
|
CCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGG > NC_000913/3103875‑3104024
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
