Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,337,996 |
C→T |
G156S (GGC→AGC) |
mlaD ← |
intermembrane phospholipid transport system, substrate binding protein MlaD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,337,996 | 0 | C | T | 100.0%
| 102.9
/ NA
| 30 | G156S (GGC→AGC) | mlaD | intermembrane phospholipid transport system, substrate binding protein MlaD |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/12); total (18/12) |
GTACCCACAGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCAT > NC_000913/3337920‑3338073
|
gTACCCACAGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCtttt < 2:586730/80‑1 (MQ=255)
gTACCCACAGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTta > 2:586772/1‑81 (MQ=255)
cacaGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTAcc < 2:586731/81‑1 (MQ=255)
acaGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCg < 1:586733/81‑1 (MQ=255)
acaGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCg < 2:586734/81‑1 (MQ=255)
acaGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCg < 1:586732/81‑1 (MQ=255)
tCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAg > 2:586773/1‑80 (MQ=255)
cAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAgg > 1:586768/1‑80 (MQ=255)
aGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGaa > 2:586764/1‑81 (MQ=255)
attattACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAAt > 1:586794/1‑81 (MQ=255)
ttattaCCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATg < 1:586735/81‑1 (MQ=255)
ttattaCCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATg < 1:586736/81‑1 (MQ=255)
attaCCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAAt > 2:586774/1‑78 (MQ=255)
ttaCCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAAt > 2:586780/1‑77 (MQ=255)
taCCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTCTTACTACCGTAAAGGAACTGACCAAt > 2:586761/1‑76 (MQ=255)
ccTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATc < 1:586737/80‑1 (MQ=255)
ggCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTc > 1:586792/1‑80 (MQ=255)
ggCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTc > 1:586791/1‑80 (MQ=255)
ggCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTc > 1:586760/1‑80 (MQ=255)
gcgcAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTcc < 1:586738/80‑1 (MQ=255)
gcagcaGCTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAg > 2:586779/1‑80 (MQ=255)
cagcTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCa > 1:586782/1‑81 (MQ=255)
agcTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCAt < 2:586739/81‑1 (MQ=255)
gcTGGCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATc < 2:586740/81‑1 (MQ=255)
ggCGCATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCg > 2:586783/1‑79 (MQ=255)
cATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACt < 1:586741/81‑1 (MQ=255)
cATCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACt > 1:586786/1‑81 (MQ=255)
aTCGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACt > 1:586787/1‑80 (MQ=255)
cGCCACTATTCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAg > 2:586784/1‑81 (MQ=255)
ttCTTATTGTCATCGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTg < 2:586742/80‑1 (MQ=255)
cGCTTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCAt > 2:586788/1‑81 (MQ=255)
|
GTACCCACAGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCAT > NC_000913/3337920‑3338073
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
