Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,539,530 |
A→G |
S1021P (TCC→CCC) |
narZ ← |
nitrate reductase Z subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,539,530 | 0 | A | G | 100.0%
| 108.6
/ NA
| 31 | S1021P (TCC→CCC) | narZ | nitrate reductase Z subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/14); total (17/14) |
CTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGC > NC_000913/1539453‑1539607
|
cTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGAc > 2:291232/1‑81 (MQ=255)
aTAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGt > 2:291241/1‑80 (MQ=255)
aTAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTg < 1:291200/81‑1 (MQ=255)
gagCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGAt < 2:291201/80‑1 (MQ=255)
cGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGa > 1:291231/1‑81 (MQ=255)
gTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGAc > 2:291247/1‑81 (MQ=255)
tCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGAcc < 2:291203/81‑1 (MQ=255)
tCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGAcc < 2:291202/81‑1 (MQ=255)
gACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGAc < 1:291204/81‑1 (MQ=255)
gACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGAc > 2:291249/1‑81 (MQ=255)
gACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGAc > 2:291235/1‑81 (MQ=255)
aCAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGAc > 1:291238/1‑80 (MQ=255)
cAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCa < 1:291206/81‑1 (MQ=255)
cAGCGTGCGCCACGGAATTAACTCATGAACGATGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCa < 2:291205/81‑1 (MQ=255)
aGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAg > 2:291250/1‑81 (MQ=255)
aGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAg < 1:291207/81‑1 (MQ=255)
aCGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTgg < 2:291208/81‑1 (MQ=255)
aCGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTg > 1:291257/1‑80 (MQ=255)
aTGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTa < 2:291209/79‑1 (MQ=255)
aTGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcg > 1:291253/1‑81 (MQ=255)
aTGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcg > 2:291239/1‑81 (MQ=255)
gAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcgcg > 1:291242/1‑81 (MQ=255)
aaCGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCgg < 2:291210/81‑1 (MQ=255)
aTCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGaa > 1:291244/1‑81 (MQ=255)
cccGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAata > 1:291254/1‑81 (MQ=255)
cgcgTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATc < 1:291211/81‑1 (MQ=255)
cgcgTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATc < 1:747107/81‑1 (MQ=255)
gcgTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATcg > 2:291245/1‑81 (MQ=255)
ataGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGaa < 1:291212/80‑1 (MQ=255)
aGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGc > 1:291240/1‑81 (MQ=255)
aGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGc > 2:291265/1‑81 (MQ=255)
|
CTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGC > NC_000913/1539453‑1539607
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
