Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,662,456 |
T→A |
L634* (TTG→TAG) |
ynfF → |
putative selenate reductase YnfF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,662,456 | 0 | T | A | 100.0%
| 61.2
/ NA
| 18 | L634* (TTG→TAG) | ynfF | putative selenate reductase YnfF |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (9/9); total (9/9) |
GATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTGGGAATTGAAAAAA > NC_000913/1662379‑1662532
|
gATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTAGaa < 2:309259/81‑1 (MQ=255)
gAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTAGAAAACACCTTc < 1:309260/81‑1 (MQ=255)
aaaaTGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTAGAAAACACCTTCggg > 2:309283/1‑81 (MQ=255)
cacTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTAt > 1:309285/1‑81 (MQ=255)
acTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTAtt > 1:309292/1‑81 (MQ=255)
ccGCGCATTCCGTGAAGATCCACAGGCAAACCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGc > 1:309296/1‑81 (MQ=255)
gcATTCCGTGAAGATCCACAGGCAAACCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAg < 2:309261/81‑1 (MQ=255)
ttCCGTGAAGATCCACAGGCAAACCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGAtt < 2:309262/81‑1 (MQ=255)
catGAAGATCCACAGGCAGACCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCa < 2:309263/79‑1 (MQ=255)
aTCCACAGGCAAACCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCt > 1:309290/1‑81 (MQ=255)
aTCCACAGGCAAACCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCt < 2:309264/81‑1 (MQ=255)
ggCAAACCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTGGGAAtt < 1:309265/81‑1 (MQ=255)
aaaCCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTGGGAATTGaa < 2:309266/81‑1 (MQ=255)
aaaCCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTGGGAATTGa > 1:309286/1‑80 (MQ=255)
aaaCCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTGGGAATTGa > 2:309287/1‑80 (MQ=255)
aCCCGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTGGGAATTGaaa > 2:309295/1‑80 (MQ=255)
ccGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTGGGAATTGaaaaaa < 2:309267/81‑1 (MQ=255)
cGTAGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTGGGAATTGaaaa > 1:309298/1‑78 (MQ=255)
|
GATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTGGGAATTGAAAAAA > NC_000913/1662379‑1662532
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
