Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,124,626 |
G→T |
A374E (GCG→GAG) |
wcaI ← |
putative colanic biosynthesis glycosyl transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,124,626 | 0 | G | T | 100.0%
| 138.2
/ NA
| 38 | A374E (GCG→GAG) | wcaI | putative colanic biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (18/20); total (18/20) |
ACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCA > NC_000913/2124550‑2124702
|
aCGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCt > 1:386016/1‑80 (MQ=255)
gTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGAc < 1:385969/81‑1 (MQ=255)
acacGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCATGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACgg < 2:385970/80‑1 (MQ=255)
cacGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGAt < 1:385971/81‑1 (MQ=255)
gTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATccc < 1:385972/81‑1 (MQ=255)
ctctTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGc > 1:386018/1‑81 (MQ=255)
tctTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGc > 1:386019/1‑80 (MQ=255)
ctTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGcca > 1:386015/1‑81 (MQ=255)
tttATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGccac > 1:386025/1‑81 (MQ=255)
tttATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGccac > 2:386030/1‑81 (MQ=255)
ttATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGccac > 2:386031/1‑80 (MQ=255)
ttATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGccac > 2:386028/1‑80 (MQ=255)
tCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAgc < 2:385973/81‑1 (MQ=255)
aGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGcc > 1:386029/1‑81 (MQ=255)
gcgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCt < 1:385974/81‑1 (MQ=255)
gcgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCt > 1:386034/1‑81 (MQ=255)
cgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTc < 1:385975/81‑1 (MQ=255)
gtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTc > 1:386020/1‑80 (MQ=255)
gtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCg < 2:385976/81‑1 (MQ=255)
gcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGAc > 1:386022/1‑81 (MQ=255)
cgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGAcc > 1:386024/1‑81 (MQ=255)
gCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTcc > 2:386026/1‑81 (MQ=255)
tatTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGt > 1:386013/1‑81 (MQ=255)
aCGTGCCACCGTGTTGTGCTTGGGCAGCAGGGGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAAc < 1:385977/81‑1 (MQ=255)
gCCACCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCa > 1:386033/1‑80 (MQ=255)
aCCGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAAcc < 1:385978/81‑1 (MQ=255)
cGTGTTGTGTTTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGc < 2:385979/81‑1 (MQ=255)
ttgtgttTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATg < 2:385981/81‑1 (MQ=255)
ttgtgttTGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATg < 1:385980/81‑1 (MQ=255)
tttGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCgg < 2:385982/81‑1 (MQ=255)
tttGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCgg < 1:385983/81‑1 (MQ=255)
tGGGCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGa < 1:385984/80‑1 (MQ=255)
gggCAGCAGGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGa > 1:386040/1‑79 (MQ=255)
cagGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTc < 2:385985/81‑1 (MQ=255)
cagGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTc < 1:385986/81‑1 (MQ=255)
agGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCg < 2:385987/81‑1 (MQ=255)
agGAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCg > 2:385988/1‑81 (MQ=255)
gAGCTCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCa < 2:622209/81‑1 (MQ=255)
|
ACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCA > NC_000913/2124550‑2124702
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
