Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F95 I1 R1	21	58.4	2051072	94.9%	1946467	140.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,204,477	G→T	intergenic (+188/‑119)	yehK → / → yehL	uncharacterized protein/putative hexameric AAA+ MoxR family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,204,477	0	G	T	100.0%	116.9 / NA	34	intergenic (+188/‑119)	yehK/yehL	uncharacterized protein/putative hexameric AAA+ MoxR family ATPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/18); total (16/18)

CAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAATCATCTGCA  >  NC_000913_3_hsa_tpiA/2204334‑2204621

cAATGGCCTGGATTATAAAGCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTtttttt                                                                                                                                            <  2:627297/150‑1 (MQ=255)

 aaTGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGtttttttt                                                                                                                                           >  2:38328/1‑150 (MQ=255)

            ttATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGa                                                                                                                                >  1:490789/1‑150 (MQ=255)

              ataAATCCCATTATCTTAATTGCAACGGGGTCAAGCACAGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATc                                                                                                                              <  1:797672/150‑1 (MQ=255)

                       aTTATCTTAATTGCAACGGGGTCCATCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCa                                                                                                                     <  1:314347/150‑1 (MQ=255)

                          aTCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGat                                                                                                                  <  2:409115/150‑1 (MQ=255)

                           tCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGata                                                                                                                 >  1:989469/1‑150 (MQ=255)

                                atggCACCGGCGTCCAGACGTGTGTATGATTCGTTCGTTGGAACACGATGTTGCACAGGCTTTGGTGTTAGGCCTTAAAATAGTAAATAAAAGAACCGGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATAttttt                                                                                                            <  2:295877/147‑1 (MQ=255)

                                aTTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATAttttt                                                                                                            <  1:38328/150‑1 (MQ=255)

                                 ttGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATAtttttt                                                                                                           >  2:699476/1‑149 (MQ=255)

                                 ttGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTc                                                                                                           >  1:771530/1‑150 (MQ=255)

                                   gCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATACAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTCCTGTGATCTGCTTTCCAGATATTTTTcta                                                                                                         >  2:255032/1‑148 (MQ=255)

                                        ggggTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGata                                                                                                                 >  1:159388/1‑137 (MQ=255)

                                        ggggTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGata                                                                                                                 <  2:159388/137‑1 (MQ=255)

                                           gTCCAGCCGTGGTTATAATACGTTCGTTAAAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAaacaa                                                                                                 <  1:381121/150‑1 (MQ=255)

                                            tCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAaacaac                                                                                                <  2:490789/150‑1 (MQ=255)

                                                  cGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATgc                                                                                          <  2:458558/150‑1 (MQ=255)

                                                   gTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATgcg                                                                                         >  1:412317/1‑150 (MQ=255)

                                                    tGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATgcgc                                                                                        <  2:599912/150‑1 (MQ=255)

                                                          aaaTGCGTTCGTAAGAAGAAGATAATGCACAGGCTGTGGTGTTAGGCGTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTCCTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACa                                                                                  <  2:962119/149‑1 (MQ=255)

                                                              tcgttcgtTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTa                                                                              <  2:921606/150‑1 (MQ=255)

                                                                    gttGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGCTATTTTTCGCTCAAACAACTAATGCGCCAAAAATTTATTgcgc                                                                        >  1:342390/1‑150 (MQ=255)

                                                                               aTGTTGCACAGCTTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTACTTTCCAGATATTTCTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCg                                                             <  1:901770/150‑1 (MQ=255)

                                                                                        aGGCTGTGGCGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTATTTTTATCTGCTTTCCAGATATTTTTCTCTCAATCATCTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCa                                                    >  2:609299/1‑150 (MQ=255)

                                                                                         ggCCGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCTCGTAAAATATCGTTTATTTCAt                                                   >  2:994696/1‑150 (MQ=255)

                                                                                                   ttAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTGACCAGCAGTTTTTTTTTTTTATTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAGTTATTGTGCGTAACATTTTTTGCGCGTAAATTATCGTTTTTTTTTTTTATACtttt                                         >  2:897857/1‑150 (MQ=255)

                                                                                                       gCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAgg                                     >  1:980529/1‑150 (MQ=255)

                                                                                                                  gTAAATAAAAGAACCTGCCTCTCCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAatatat                          <  1:612264/150‑1 (MQ=255)

                                                                                                                   taaataaaAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTCTTTCATTCATACATTTCAGGGATGAatatatt                         >  2:861175/1‑149 (MQ=255)

                                                                                                                   taaataaaAGAACCTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATg                         >  1:727790/1‑150 (MQ=255)

                                                                                                                                cTGCCTCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGaata            >  2:630176/1‑150 (MQ=255)

                                                                                                                                   ccTCACCAGCATTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAatc         <  2:412317/150‑1 (MQ=255)

                                                                                                                                     tCACCAGCAGTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAatcac       >  1:604108/1‑149 (MQ=255)

                                                                                                                                          agcagTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAATCATCTGCa  <  2:272540/150‑1 (MQ=255)

                                                                                                                                          agcagTTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAATCATCTGCa  <  1:255032/150‑1 (MQ=255)

CAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAATCATCTGCA  >  NC_000913_3_hsa_tpiA/2204334‑2204621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A