Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F74 I1 R1
|
22 |
34.8 |
1210890 |
93.9% |
1137025 |
142.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,430,917 |
C→G |
intergenic (‑154/+105) |
cvpA ← / ← dedD |
colicin V production protein/membrane‑anchored periplasmic protein involved in septation |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,430,917 | 0 | C | G | 93.3%
| 83.9
/ 0.3
| 30 | intergenic (‑154/+105) | cvpA/dedD | colicin V production protein/membrane‑anchored periplasmic protein involved in septation |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base G (14/14); total (15/15) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01 |
CCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGC > NC_000913_3_hsa_tpiA/2430771‑2431059
|
ccACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATggg < 1:569418/149‑1 (MQ=255)
tACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCgt < 1:73691/149‑1 (MQ=255)
gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTc > 1:341116/1‑149 (MQ=255)
cTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGt > 2:515914/1‑149 (MQ=255)
tGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTGCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCCGCGTa > 1:400730/1‑149 (MQ=255)
gcgcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt > 2:433959/1‑149 (MQ=255)
aCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa < 2:38972/149‑1 (MQ=255)
agaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATCCGTGTCGGATGCGCCGTAAACGCCTTATCCCACCTAcc > 2:414985/1‑148 (MQ=255)
tttGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGATTCTACCCCTGCg > 1:407631/1‑149 (MQ=255)
tGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTa < 1:439981/149‑1 (MQ=255)
gCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAg < 1:363083/149‑1 (MQ=255)
aGGGAGTGCCTTCCCTCGCATGAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTGTTATGCCTTTGCGGCATGGGGCATTGCGTGTCGGATGCGGCGTAAACGCCTTATTCGACCTACGGTTCTACCCCTGCGTAGGCCt < 2:585893/149‑1 (MQ=38)
cttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGTGGCGTAAACGCCTTATCCGACCTACGGTTCCACCCCTCCGTAGGCCTCATAAGAcc > 2:167713/1‑148 (MQ=39)
cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc < 1:262359/133‑1 (MQ=255)
cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc > 2:262359/1‑133 (MQ=255)
cATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGt < 2:575536/149‑1 (MQ=255)
aaaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCa < 2:317149/149‑1 (MQ=255)
aaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg > 1:405616/1‑149 (MQ=255)
gcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAc > 2:597253/1‑123 (MQ=255)
gcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAc < 1:597253/123‑1 (MQ=255)
gAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTa < 1:556545/149‑1 (MQ=255)
aaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTaa < 2:545946/149‑1 (MQ=255)
aaCGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcgc < 2:573414/110‑1 (MQ=255)
aaCGCCATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcgc > 1:573414/1‑110 (MQ=255)
ccccATCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCGGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGtat < 2:99716/147‑1 (MQ=255)
tCGACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGccc > 1:191753/1‑149 (MQ=255)
gACTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGGGTATAGcccgt > 1:152845/1‑147 (MQ=255)
aCTTTTTATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGCCGCATCAGGCAAGACCGTATTAATTCGGCGTATACACCAtt > 1:301323/1‑149 (MQ=255)
tttttATGCCTTTGCGGCATGGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGGCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTAc > 2:549553/1‑149 (MQ=255)
tttttAAGCCTTTCAGGGATGGGGCAATGCGTGTGGGATGGGGCGTAAACCCCTTCCCAGACCTACGGTACACACCCTGAGTATGCCCGATAAGACGCGCCCGGCTCGCTTGTGGCGGTTCGGGGTTAGTTCGGCGTATAGCCCATTAc < 2:120889/149‑1 (MQ=255)
cGGCATGGGGCAATGCGTGCCGGAGGGGGCGTAAACGCCTCATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc < 2:156333/149‑1 (MQ=39)
|
CCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGC > NC_000913_3_hsa_tpiA/2430771‑2431059
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
