Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F101 I1 R1	23	42.5	1457892	94.3%	1374792	144.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	3,316,538	T→G	Q330P (CAG→CCG)	nusA ←	transcription termination/antitermination L factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	3,316,538	0	T	G	100.0%	142.6 / NA	42	Q330P (CAG→CCG)	nusA	transcription termination/antitermination L factor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (26/16); total (26/16)

AGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAA  >  NC_000913_3_hsa_tpiA/3316395‑3316680

aGCCATCATCTACCAGAACAGGCGCGAAGTCTTCGTCGATGTCGAGATACTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGaa                                                                                                                                           <  1:286013/149‑1 (MQ=255)

  ccTTCTTCTACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGc                                                                                                                                         >  1:510352/1‑149 (MQ=255)

   cttcttCTACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATGGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGcc                                                                                                                                        >  1:148841/1‑149 (MQ=255)

     tcttctACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAg                                                                                                                                      <  1:416712/149‑1 (MQ=255)

      cttctACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGa                                                                                                                                     >  2:273109/1‑149 (MQ=255)

       ttctACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGAc                                                                                                                                    >  2:32986/1‑149 (MQ=255)

           aCCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCAc                                                                                                                                >  1:233218/1‑149 (MQ=255)

              aGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGtt                                                                                                                             >  1:487536/1‑149 (MQ=255)

              aGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCATGATGCTTAGCCTGCAGGTCGTCAACGGTCAGCACTGTGAGGTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGtt                                                                                                                             >  2:699435/1‑149 (MQ=255)

                  cAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGa                                                                                                                         <  1:273109/149‑1 (MQ=255)

                    gTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACCCACGTTCTGAcc                                                                                                                       >  2:482842/1‑149 (MQ=255)

                        cgAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTa                                                                                                                   >  2:497565/1‑149 (MQ=255)

                                           gagcTATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTGCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCACATCGCCTGCGCCa                                                                                                >  2:648430/1‑149 (MQ=255)

                                           gagaTATTTGGTGAAGGTGTCGATCGCTACGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCCGCCGCACGATCTGACAGGTACCGCCAATCGCGCGCGccg                                                                                                >  2:248137/1‑148 (MQ=255)

                                            agaTATTTGGTGAAGGTGTCGATCGCTGCGTACGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCCACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAACAAGACGCACGTTCTGACCGTTACGGCCAATAGCCTgcgcacg                                                                                               >  2:415283/1‑146 (MQ=255)

                                                 tttGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTAc                                                                                          >  1:337997/1‑149 (MQ=255)

                                                  ttGGTGATGGTTTTGGTCGCTGCGTGCGCTTCCGCCTGATGCTCAGCCTGCAGGTCGTCAACCGTCATGACGTTGAGTTCCCCACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTAcc                                                                                         <  2:194992/149‑1 (MQ=255)

                                                     gTGAAGGGGTCGATCGCTGCGTGCGCTTCCGTCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGc                                                                                      <  2:165592/149‑1 (MQ=255)

                                                           gtgtCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTCCCGGCTTCAAc                                                                                >  2:574226/1‑149 (MQ=255)

                                                              tCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGc                                                                             <  2:423210/149‑1 (MQ=255)

                                                                   cGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGatat                                                                        <  2:487536/149‑1 (MQ=255)

                                                                         gtgcgCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATgg                                                                  >  1:421418/1‑149 (MQ=255)

                                                                               gtCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGtt                                                            <  2:99113/148‑1 (MQ=255)

                                                                                    ccTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCt                                                       <  1:441905/149‑1 (MQ=255)

                                                                                      tGATTCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTc                                                     <  2:413089/149‑1 (MQ=255)

                                                                                        aTGCTTAGCCCACATGGCGTCAAAGGTCATCACGTTGAGTTCCGAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGAGATCCATGGTGTGTTTATCTTCAt                                                   <  2:86212/149‑1 (MQ=255)

                                                                                          gCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATcc                                                 >  2:573192/1‑149 (MQ=255)

                                                                                            ttAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccac                                               <  2:720842/149‑1 (MQ=255)

                                                                                                 cTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGa                                          <  1:580351/149‑1 (MQ=255)

                                                                                                      ggTCGCCAACGGTCATCACGTTGAGTTCGCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCTAATCGCTTGCGCCAGGTTTCCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGaa                                     <  2:324047/149‑1 (MQ=255)

                                                                                                       gtcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAg                                    <  1:291208/149‑1 (MQ=255)

                                                                                                        tcgtcAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAgcaca                                   >  2:183340/1‑145 (MQ=255)

                                                                                                                 gTCATGCCGTTGAGTTCCCAACCGCGCAGCGGCGAAGCCAGACGCACGTTCGGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGc                          <  2:570229/149‑1 (MQ=255)

                                                                                                                       aCGTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACGACGATAGAAGCAACGTCTGCCGGTGc                    >  1:92530/1‑149 (MQ=255)

                                                                                                                               ttCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCAACCCCGCTAGAATCAGCGTCTGCCCGTGCCATTGCGt            >  2:13425/1‑149 (MQ=255)

                                                                                                                               ttCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGAACATTGTTGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGt            >  1:537178/1‑149 (MQ=255)

                                                                                                                                  ccAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGAACGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATCGAAGCAACGACTGCCGGTGCCATTGCGCTaa         >  1:54705/1‑149 (MQ=255)

                                                                                                                                   cAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAAt        >  1:567802/1‑149 (MQ=255)

                                                                                                                                    aaCCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATc       >  2:550540/1‑149 (MQ=255)

                                                                                                                                      ccGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTTATCAc     >  2:422812/1‑149 (MQ=255)

                                                                                                                                         cTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGaa  >  1:619571/1‑149 (MQ=255)

                                                                                                                                         cTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGaa  >  2:515652/1‑149 (MQ=255)

AGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAA  >  NC_000913_3_hsa_tpiA/3316395‑3316680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A