Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A22 F59 I1 R1	12	40.7	1542742	93.9%	1448634	144.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	3,734,142	C→A	V141V (GTC→GTA)	xylH →	D‑xylose ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	3,734,142	0	C	A	100.0%	110.8 / NA	38	V141V (GTC→GTA)	xylH	D‑xylose ABC transporter permease
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (25/13); total (25/13)

CTGTTAGGTGGCGTCGCGGCGATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGC  >  NC_000913_3_bme_pgi/3733996‑3734286

cTGTTAGGTGGCGTCGTAGCGATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAAc                                                                                                                                                <  1:748289/149‑1 (MQ=255)

             tCGCGGCGATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCCCTGGCATg                                                                                                                                   >  2:227572/1‑149 (MQ=255)

               gcggcgATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGCCTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGtt                                                                                                                                 <  1:539372/149‑1 (MQ=255)

                 ggcgATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTGCCGTCATTTATGGTAACCCTCGCGGGCATGTTgg                                                                                                                               >  1:354289/1‑149 (MQ=255)

                          tgACGTCTGGTTTGGGTGGTCATTGCTATTTTGTATCATTGGGACGCTGGTTGCGGGACTGCCTCGCGGTGCCTGGGACGGATGGTGGGTCGCGTGCAGTAAAGGCCCTTCGTTTATTGTAACCCTCGCGGGCATGTTGGCATTTcgcg                                                                                                                      <  2:513535/149‑1 (MQ=255)

                          tgACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTcgcg                                                                                                                      <  1:225456/149‑1 (MQ=255)

                            cccgcgggTTGGACTGGCCTGTGCCAATTACCTTCATTGTGGCGCTGGTCCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGc                                                                                                                    <  2:595035/143‑1 (MQ=255)

                              gTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCAt                                                                                                                  >  1:124886/1‑149 (MQ=255)

                                   gTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCa                                                                                                             >  2:168009/1‑149 (MQ=255)

                                        gCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTTCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCGTATATTGTAAACTTCTCGGGGAAGTTGCAATTTAGCGGAATACTCATTgcg                                                                                                        >  2:500171/1‑147 (MQ=255)

                                        gCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGc                                                                                                        >  1:717188/1‑149 (MQ=255)

                                         cTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACACTCACGGGCATGTTGGCATTTCGCGGCATACCCATTGGCa                                                                                                       >  2:164862/1‑149 (MQ=255)

                                                 tGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATTCTCATTGGCATCACCAAc                                                                                               <  1:285020/149‑1 (MQ=255)

                                                    cACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGc                                                                                            >  1:193877/1‑149 (MQ=255)

                                                     aCTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAGAGTCCCTTCATGTGTTGTAACGGTCGCGGGCGTGTTGTCATTTCGCGGCACACACATTGGCCTCGCCGACGGCa                                                                                           >  2:327833/1‑149 (MQ=255)

                                                           catcatTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTg                                                                                     >  1:24704/1‑149 (MQ=255)

                                                            atcacTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGCTGGTGGGTCGCGTACCGTAAAGTCCCTTCATCCATTGTACCCCTCGGGGGCATGTTGGCATTTCGCGGCGTACTCATTGGCAGCACCCACGGCaacaccgt                                                                                    >  2:585920/1‑142 (MQ=255)

                                                                     aCGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCccac                                                                           <  2:193877/149‑1 (MQ=255)

                                                                     aCGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCccac                                                                           >  2:123577/1‑149 (MQ=255)

                                                                                gggACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGa                                                                >  1:131651/1‑149 (MQ=255)

                                                                                    cTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTc                                                            >  2:20674/1‑149 (MQ=255)

                                                                                    cTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCGTCATATATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCCCCGGGATGTc                                                            >  1:196106/1‑149 (MQ=255)

                                                                                             ggTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTgg                                                   <  2:639835/149‑1 (MQ=255)

                                                                                                       aCGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATGTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTAt                                         <  1:548736/149‑1 (MQ=255)

                                                                                                        cGGATTGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAATCCTCGCGTGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTAtc                                        <  1:89/149‑1 (MQ=255)

                                                                                                           aTGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTcc                                     >  2:559459/1‑149 (MQ=255)

                                                                                                                     gcgTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATACCCCACCAGCGCCGCGATGTCACAAATTGGGCAAACCTATCTCcccccccgccc                           >  2:110088/1‑144 (MQ=255)

                                                                                                                           cGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTCCCGGCtt                     >  2:332479/1‑149 (MQ=255)

                                                                                                                                aGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatca                >  2:523972/1‑149 (MQ=255)

                                                                                                                                     cTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGgc           >  1:117263/1‑149 (MQ=255)

                                                                                                                                      ttCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGCGTTCATCATTGgct          >  1:332114/1‑148 (MQ=255)

                                                                                                                                       tCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGCCTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAGGCTATCTCCCCGCCAGTACCGGCTTCGTCATTGgcgc         >  1:172918/1‑149 (MQ=255)

                                                                                                                                         aTTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAACTGGGCAAAGCTAtctc                                      >  2:162269/1‑118 (MQ=255)

                                                                                                                                         aTTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAACTGGGCAAAGCTAtctc                                      <  1:162269/118‑1 (MQ=255)

                                                                                                                                          tttATTGTAACCCTCGCGGGCATGTTGGCATTTCACGCCAGATTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCCCAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGACTTCATCATTGgcactct      >  2:165585/1‑144 (MQ=255)

                                                                                                                                            tATTGTAACCCGCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTg    <  1:318728/149‑1 (MQ=255)

                                                                                                                                             aTTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTgg   <  1:594132/149‑1 (MQ=255)

                                                                                                                                              ttGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATAATCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGc  >  1:303960/1‑149 (MQ=255)

CTGTTAGGTGGCGTCGCGGCGATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGC  >  NC_000913_3_bme_pgi/3733996‑3734286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A