Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F29 I1 R1 13 69.3 2756640 93.6% 2580215 126.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 3,316,893 C→T E212K (GAA→AAA)  nusA ← transcription termination/antitermination L factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi3,316,8930CT98.8% 308.0 / NA 86E212K (GAA→AAA) nusAtranscription termination/antitermination L factor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (40/45);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCTTCGC  >  NC_000913_3_bme_pgi/3316748‑3317036
                                                                                                                                                 |                                                                                                                                               
tGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCATTTTAATa                                                                                                                                             <  2:846695/150‑1 (MQ=255)
tGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATa                                                                                                                                             <  3:67950/150‑1 (MQ=255)
   acacGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACCAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACgg                                                                                                                                          <  1:524144/150‑1 (MQ=255)
     acGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGaa                                                                                                                                        <  2:66494/150‑1 (MQ=255)
     acGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGaa                                                                                                                                        <  3:103109/150‑1 (MQ=255)
       gcgcgcCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACa                                                                                                                                      >  2:549449/1‑150 (MQ=255)
             cACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGa                                                                                                                                <  4:37522/150‑1 (MQ=255)
               cGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAATAGTTCGATc                                                                                                                              <  3:400203/150‑1 (MQ=255)
               cGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATc                                                                                                                              <  1:86440/150‑1 (MQ=255)
                gCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCa                                                                                                                             <  4:370105/150‑1 (MQ=255)
                 cATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAg                                                                                                                            <  3:166133/150‑1 (MQ=255)
                    aCCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCAt                                                                                                                         >  1:256051/1‑150 (MQ=255)
                       tACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTc                                                                                                                      >  4:109329/1‑150 (MQ=255)
                          gCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCgg                                                                                                                   >  2:833209/1‑150 (MQ=255)
                           cAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGc                                                                                                                  <  3:109329/150‑1 (MQ=255)
                                aCCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGCACTCTGGCCCTTCTCTTCGGCACAGTTCGCTCAGCATTTCCGGCTTGGa                                                                                                             >  3:413270/1‑150 (MQ=255)
                                  cTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAAc                                                                                                           >  4:160614/1‑150 (MQ=255)
                                    aCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTACTTCGCCGATTTCTGGCACTTTAATACGGAAAAGTTCGATCAGCATTTCCGGCTTGGAACGa                                                                                                         <  3:221740/150‑1 (MQ=255)
                                        gATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGa                                                                                                     <  2:256051/150‑1 (MQ=255)
                                        gATCGATACGTCTATCGTTGTTTTTCACCGCGATTTTCGCACGAGAACCCGGAGCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAAGACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGa                                                                                                     <  4:251088/150‑1 (MQ=255)
                                         aTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAAt                                                                                                                                              <  4:73416/108‑1 (MQ=255)
                                         aTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAAt                                                                                                                                              >  3:73416/1‑108 (MQ=255)
                                         aTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGAc                                                                                                    >  1:187005/1‑150 (MQ=255)
                                             aTACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAAc                                                                                                <  3:452405/150‑1 (MQ=255)
                                                 gTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGtt                                                                                            >  4:315134/1‑150 (MQ=255)
                                                  tttATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGaa                                                                                                                                        <  2:303786/105‑1 (MQ=255)
                                                  tttATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGaa                                                                                                                                        >  1:303786/1‑105 (MQ=255)
                                                    tATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTgcg                                                                                         >  2:350750/1‑150 (MQ=255)
                                                        gTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGcc                                                                                     >  2:213622/1‑150 (MQ=255)
                                                        gTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGcc                                                                                     >  2:27891/1‑150 (MQ=255)
                                                         ttGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCa                                                                                    >  3:352301/1‑150 (MQ=255)
                                                         ttGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCa                                                                                    >  2:854955/1‑150 (MQ=255)
                                                         ttGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCa                                                                                    >  3:376273/1‑150 (MQ=255)
                                                           ggTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCAc                                                                                   >  1:147098/1‑149 (MQ=255)
                                                           ggTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCAc                                                                                   <  2:147098/149‑1 (MQ=255)
                                                            gTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCAcgc                                                                                 <  3:109761/150‑1 (MQ=255)
                                                              tttCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACgcgc                                                                               <  1:833209/150‑1 (MQ=255)
                                                              tttCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACgcgc                                                                               >  3:133470/1‑150 (MQ=255)
                                                                    cgcgATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTcc                                                                                                                     <  4:299850/106‑1 (MQ=255)
                                                                    cgcgATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTcc                                                                                                                     >  3:299850/1‑106 (MQ=255)
                                                                    cgcgATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCgg                                                                         >  4:325607/1‑150 (MQ=255)
                                                                     gcgATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCggg                                                                        <  1:27891/150‑1 (MQ=255)
                                                                     gcaaTTTTAGCAAGAGAACCCGGATGTTTATTCACTGCTTTAGGTTCAATCACTTCTTCGCCGATTTTTACCACTTTACTACACATAACTTTCATCAGCTTTTCCTGCTTGGAAGGCGCGACCTACAGTTGCGCGCCACGCGCTTCCggg                                                                        <  4:240555/147‑1 (MQ=255)
                                                                       gATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACgg                                                                                                                                          <  2:459040/82‑1 (MQ=255)
                                                                       gATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACgg                                                                                                                                          >  1:459040/1‑82 (MQ=255)
                                                                           ttCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTCTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGCGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACg                                                                  <  4:325759/150‑1 (MQ=255)
                                                                               cACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTgcgcg                                                                                       >  2:548309/1‑125 (MQ=255)
                                                                               cACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTgcgcg                                                                                       <  1:548309/125‑1 (MQ=255)
                                                                                aCGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATa                                                             <  1:32364/150‑1 (MQ=255)
                                                                                  gagaACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATaga                                                           <  4:133470/150‑1 (MQ=255)
                                                                                  gagaACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATaga                                                           >  1:542644/1‑150 (MQ=255)
                                                                                     aaCCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCa                                                        <  3:160614/150‑1 (MQ=255)
                                                                                       cccGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGAt                                                                                                                               >  1:781148/1‑77 (MQ=255)
                                                                                       cccGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGAt                                                                                                                               <  2:781148/77‑1 (MQ=255)
                                                                                       cccGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACg                                                      <  1:350750/150‑1 (MQ=255)
                                                                                       cccGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACg                                                      <  4:224800/150‑1 (MQ=255)
                                                                                        ccGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGc                                                     >  1:836790/1‑150 (MQ=255)
                                                                                          ggATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCa                                                   >  1:445607/1‑150 (MQ=255)
                                                                                           gATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCAc                                                  <  4:351863/150‑1 (MQ=255)
                                                                                               gcgAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAg                                                                                                        >  3:216047/1‑92 (MQ=255)
                                                                                               gcgAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAg                                                                                                        <  4:216047/92‑1 (MQ=255)
                                                                                                 gAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAAcg                                             <  2:328504/149‑1 (MQ=255)
                                                                                                 gAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAAcg                                             >  1:328504/1‑149 (MQ=255)
                                                                                                    ccGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCAc                                                                                   <  1:602223/108‑1 (MQ=255)
                                                                                                    ccGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCAc                                                                                   >  2:602223/1‑108 (MQ=255)
                                                                                                            ttAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAggg                                 >  3:142153/1‑150 (MQ=255)
                                                                                                             tAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGc                                >  1:110131/1‑150 (MQ=255)
                                                                                                                     aTCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGttt                        <  2:542644/150‑1 (MQ=255)
                                                                                                                        aCTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCa                     >  3:6578/1‑150 (MQ=255)
                                                                                                                          ttcttcGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGt                                                                                                       <  2:738300/66‑1 (MQ=255)
                                                                                                                          ttcttcGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGt                                                                                                       >  1:738300/1‑66 (MQ=255)
                                                                                                                             ttcGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCgg                <  2:12136/150‑1 (MQ=255)
                                                                                                                               cGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGgca              >  1:687671/1‑150 (MQ=255)
                                                                                                                                gCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATa                                                             <  2:443162/102‑1 (MQ=255)
                                                                                                                                gCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATa                                                             >  1:443162/1‑102 (MQ=255)
                                                                                                                                 ccGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGgcagc            >  1:502187/1‑150 (MQ=255)
                                                                                                                                 ccGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGgcagc            >  4:379021/1‑150 (MQ=255)
                                                                                                                                  cGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACAAACAGTTGCGCGCCACGCGCTTCCGGGCGGACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGgcagca           >  3:129500/1‑150 (MQ=255)
                                                                                                                                       tCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCt      <  4:6578/150‑1 (MQ=255)
                                                                                                                                         tGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCgg                                                                         >  2:459668/1‑81 (MQ=255)
                                                                                                                                         tGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCgg                                                                         <  1:459668/81‑1 (MQ=255)
                                                                                                                                         tGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCgg                >  2:128677/1‑138 (MQ=255)
                                                                                                                                         tGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCgg                <  1:128677/138‑1 (MQ=255)
                                                                                                                                           gCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCTTcgc  <  2:687671/150‑1 (MQ=255)
                                                                                                                                             aCTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa                                                               <  3:244156/87‑1 (MQ=255)
                                                                                                                                             aCTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGaa                                                               >  4:244156/1‑87 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCTTCGC  >  NC_000913_3_bme_pgi/3316748‑3317036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A