Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F41 I1 R1
|
15 |
48.1 |
1673764 |
93.6% |
1566643 |
143.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_bme_pgi |
3,991,839 |
G→A |
W229* (TGG→TGA) |
cyaA → |
adenylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_bme_pgi | 3,991,839 | 0 | G | A | 98.3%
| 177.7
/ NA
| 58 | W229* (TGG→TGA) | cyaA | adenylate cyclase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (24/33); total (25/33) |
| Fisher's exact test for biased strand distribution p-value = 4.31e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACC > NC_000913_3_bme_pgi/3991693‑3991984
|
ctgctgCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACt > 2:414826/1‑149 (MQ=255)
gctgctTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTgg > 2:660310/1‑149 (MQ=255)
gctcctTGACGAATTTTAGCGTACCGCCGTGCGTCGCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTgg < 1:273801/149‑1 (MQ=255)
cagAATTTTATCGTACGGATGTTCTTTTCGCGGGTAAGCGTATTTTGTGGAATACGGTGCCGTGCGACGAAGAAGAGGATAACCACGACTATGTTATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAAGGACTGGATCTCGGt < 2:11733/147‑1 (MQ=255)
gAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCGGACGCCAAATGAATGGCTGGATCTCGGTgg > 1:402744/1‑149 (MQ=255)
gAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGACGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTgg < 1:696398/149‑1 (MQ=255)
gtttATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCtt < 1:522297/148‑1 (MQ=255)
ttATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTaa < 1:585147/149‑1 (MQ=255)
tgagTATCGCGGGGCGCCTCGCTTGTAAGTGTCTTCTGTGGATTCTGGTGTCGTGCGACGAAGAAGAGAATTACGCCGGCCAGCTGATGACGCTTTTCGCGCAGGTCGTGCTGACGCCAAATCAAGGACTGGATCTCGGTGGCTTAAGc < 2:733663/146‑1 (MQ=255)
aTCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGCCGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGc > 1:324354/1‑149 (MQ=255)
gTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTgg > 2:368936/1‑139 (MQ=255)
gTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTgg < 1:368936/139‑1 (MQ=255)
gattcGCCGGGACGCGGAGACTGTGGAAGATGGTGCCGGGCGACGAAGGAGAGTATTACGACGACGATGTGAGGACGCTTTACGCGCAGGGCGCGCTGACGCCAAATGGCTGACTGTAGCTCGGTGGCTTAAGCTCGGTTTCTGCTGaa < 2:806895/146‑1 (MQ=255)
gTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGaa < 1:765357/149‑1 (MQ=255)
cGCCGGTAAGCGTTTGCTGTGTAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGt < 2:488117/149‑1 (MQ=255)
gCCGGTAAGCGTATTCGGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTGTCTGCTGAAGAGTa < 1:481279/149‑1 (MQ=255)
tcGGTAAGCGGATTCTGTCGAATATGGGGCCGTGCGACGTAGAAGAGTATTACGACGACTATGTGATGACGCTTTACGCTCAGGGCGTGCTGACGCCAAATGAATTACTGGATCTCGGTGGCTCAAGCTCGCTTTCTGCTGAAGAGTAc < 1:636600/148‑1 (MQ=255)
gTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACttt < 1:687007/149‑1 (MQ=255)
cGTAGTCTGCGGAATATGGTGCCGTGGGCCGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGTTCTCGGTGGCTTTAGCTCGCTCTCTGCTGAAGAGTACTTTGGTGc < 1:662982/149‑1 (MQ=255)
tATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCa < 1:829693/149‑1 (MQ=255)
tATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTGAAGTACCCTTTCTGCTGAAGATCGCTTTGGTcccg > 2:809741/1‑148 (MQ=255)
tgtgGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCCGCCtt > 1:259186/1‑149 (MQ=255)
gtGGTGCCGTGCGTCGAAGAAGAGCATTCCGCCGACTTTGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGAAAGct < 2:432073/148‑1 (MQ=255)
tGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTACGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTgtcggcgc > 2:513102/1‑142 (MQ=255)
ggTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGccct > 2:485590/1‑149 (MQ=255)
gTGCCGTGCGACGAAGAAGACCATTACGCCGACTATGTGATGACGCTTTACGCGCAGGGCGTGCGGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTa < 1:74445/149‑1 (MQ=255)
tGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTAc > 1:47504/1‑149 (MQ=255)
gTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAACCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAag‑a < 1:553499/149‑1 (MQ=255)
gacgaAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTAt > 1:220234/1‑149 (MQ=255)
acgaAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATc > 2:546830/1‑149 (MQ=255)
gaagaaGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATCAATGACTGGATCTCGGTGGCTTTAGCGCGCTTTCTGCGGAAGAGTATTTTGGCGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGa > 2:421293/1‑149 (MQ=255)
aagaGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCt > 2:17160/1‑91 (MQ=255)
aagaGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCt < 1:17160/91‑1 (MQ=255)
aTTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCCGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATAc > 2:221466/1‑149 (MQ=255)
ttACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGaa < 2:183628/94‑1 (MQ=255)
ttACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGaa > 1:183628/1‑94 (MQ=255)
ttACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACa > 1:178607/1‑149 (MQ=255)
cgacgaCTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAg < 2:477338/149‑1 (MQ=255)
cgacgaCTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAg < 1:546830/149‑1 (MQ=255)
gacgacTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAGG‑AGAATCGGTTCCCAATACaaacc > 1:189647/1‑147 (MQ=255)
gacgacTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGc > 1:724466/1‑149 (MQ=255)
acgacTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCg < 1:775462/149‑1 (MQ=255)
cgacTATGGGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTGTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCgg < 1:129610/149‑1 (MQ=255)
gacTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTAc < 1:830684/120‑1 (MQ=255)
gacTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTAc > 2:830684/1‑120 (MQ=255)
acTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTa < 1:458534/149‑1 (MQ=255)
gcgcAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGa > 2:618598/1‑149 (MQ=255)
cAGGGCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGc < 2:220234/149‑1 (MQ=255)
gCGTGCTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTAt > 2:329793/1‑149 (MQ=255)
ccgACGCCAAATGAATGACGGGGTTTTGGTGGCTTAAGCTCGCTTTCTGCTGAAGGGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTg < 2:324354/147‑1 (MQ=255)
cTGACGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACCAGCATCATTG‑TGCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTg > 1:404225/1‑149 (MQ=255)
cgaagCCACATGAATGACTGGTTTTCGGTGGCGTAAGCGCGCTTTCTGCGGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTAAAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCGGGAAGCCTATTACTgg < 2:371461/145‑1 (MQ=255)
aCGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTcc > 1:569512/1‑99 (MQ=255)
aCGCCAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTcc < 2:569512/99‑1 (MQ=255)
gCCAAATCAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAAt < 1:364919/149‑1 (MQ=255)
ccAAATGAATGACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATa < 1:340235/149‑1 (MQ=255)
aatgaatgACTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATAccc < 1:471492/149‑1 (MQ=255)
atgACTGGATCTCGGTGGCCTAAGCTCCCTTTCAGATGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTATACAAG‑AGTATCGATTCCCCATACAAAGAGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAAcc > 2:524598/1‑149 (MQ=255)
|
CTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAG‑AGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACC > NC_000913_3_bme_pgi/3991693‑3991984
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
