Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F74 I1 R1
|
15 |
62.6 |
2301892 |
95.0% |
2186797 |
133.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_bme_pgi |
4,159,046 |
T→G |
L186R (CTG→CGG) |
oxyR → |
oxidative and nitrosative stress transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_bme_pgi | 4,159,046 | 0 | T | G | 98.0%
| 177.2
/ ‑5.8
| 52 | L186R (CTG→CGG) | oxyR | oxidative and nitrosative stress transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (29/21); minor base A (0/1); total (29/23) |
| Fisher's exact test for biased strand distribution p-value = 4.31e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCA > NC_000913_3_bme_pgi/4158904‑4159188
|
ggCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAggg > 4:197699/1‑150 (MQ=255)
cAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGa > 4:312418/1‑150 (MQ=255)
aTTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAActgc < 4:306942/150‑1 (MQ=255)
gTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATg < 1:623819/150‑1 (MQ=255)
cgcgCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGa > 2:80298/1‑150 (MQ=255)
cgcTGGTAAAAGAGAGCGAAGCATTCATTGAAGGGCCGTTGTTGGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCAAACCGCGAATGCGTACCGATGGCCGAAAAAAAAAAAAAAAAACTGCTGATGCTGGAAGATg < 4:96761/150‑1 (MQ=255)
gAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTg > 1:550564/1‑150 (MQ=255)
agagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGttt > 2:495973/1‑150 (MQ=255)
agCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCGCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcgc < 4:360461/150‑1 (MQ=255)
agCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcgc < 3:405372/150‑1 (MQ=255)
attGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATg > 1:652372/1‑150 (MQ=255)
aaGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGtt > 2:320810/1‑150 (MQ=255)
aGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt < 3:197699/150‑1 (MQ=255)
aGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt < 1:120846/150‑1 (MQ=255)
cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt > 2:143626/1‑150 (MQ=255)
tttGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGaa < 1:708949/150‑1 (MQ=255)
ttGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAg > 2:296035/1‑150 (MQ=255)
gaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg > 3:225117/1‑150 (MQ=255)
gTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGTCGGATGa < 2:704931/150‑1 (MQ=255)
tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa < 4:118371/150‑1 (MQ=255)
tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa < 1:264650/150‑1 (MQ=255)
ctatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacac > 2:158008/1‑150 (MQ=255)
atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac < 3:312418/150‑1 (MQ=255)
atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTcc > 2:618786/1‑150 (MQ=255)
tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATc > 1:187588/1‑98 (MQ=255)
tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATc < 2:187588/98‑1 (MQ=255)
aaGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt > 4:169479/1‑95 (MQ=255)
aaGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt < 3:169479/95‑1 (MQ=255)
aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgcgc < 2:15336/150‑1 (MQ=255)
aTCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGa < 2:550564/150‑1 (MQ=255)
aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa > 1:431697/1‑124 (MQ=255)
aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa < 2:431697/124‑1 (MQ=255)
aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCa > 1:513965/1‑150 (MQ=255)
ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGc < 2:509130/150‑1 (MQ=255)
cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGcc > 3:184273/1‑150 (MQ=255)
cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGcc > 2:12993/1‑150 (MQ=255)
gTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCt < 1:618786/150‑1 (MQ=255)
gCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt < 2:610571/95‑1 (MQ=255)
gCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt > 1:610571/1‑95 (MQ=255)
gCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg < 2:99319/112‑1 (MQ=255)
gCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg > 1:99319/1‑112 (MQ=255)
gAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg > 3:433205/1‑132 (MQ=255)
gAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg < 4:433205/132‑1 (MQ=255)
gAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAc > 3:34963/1‑150 (MQ=255)
cgcgAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg > 4:4278/1‑150 (MQ=255)
gcgAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc > 4:383831/1‑150 (MQ=255)
tACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg > 1:136272/1‑150 (MQ=255)
cGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg > 2:121654/1‑150 (MQ=255)
aTGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg > 2:382502/1‑88 (MQ=255)
aTGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg < 1:382502/88‑1 (MQ=255)
aTGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTACGCAACATGGTggcg > 1:309644/1‑150 (MQ=255)
gCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa > 4:371840/1‑150 (MQ=255)
|
GGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCA > NC_000913_3_bme_pgi/4158904‑4159188
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
