Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F60 I1 R1	18	74.2	2511834	99.1%	2489227	137.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,535,940	A→T	D215E (GAT→GAA)	ompR ←	DNA‑binding transcriptional dual regulator OmpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,535,940	0	A	T	100.0%	187.4 / NA	54	D215E (GAT→GAA)	ompR	DNA‑binding transcriptional dual regulator OmpR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (26/28); total (26/28)

AGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGT  >  NC_000913/3535805‑3536072

aGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTtctt                                                                                                                                  <  1:495055/140‑1 (MQ=255)

           aGCAATAACGTCCGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATg                                                                                                                       >  2:218638/1‑140 (MQ=255)

           aGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATg                                                                                                                       <  1:289241/140‑1 (MQ=255)

             cAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATgcg                                                                                                                     >  1:830301/1‑140 (MQ=255)

                 aaCGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGgcgc                                                                                                                 >  2:922915/1‑140 (MQ=255)

                     tACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGAc                                                                                                             >  1:1214462/1‑140 (MQ=255)

                     tACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGGACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGAc                                                                                                             >  1:862052/1‑140 (MQ=255)

                          gCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGaa                                                                                                        <  2:1109405/140‑1 (MQ=255)

                          gCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGaa                                                                                                        >  1:438224/1‑140 (MQ=255)

                             aaTGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATc                                                                                                     <  1:864967/140‑1 (MQ=255)

                             aaTGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATc                                                                                                     >  2:1181073/1‑140 (MQ=255)

                               tGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTg                                                                                                   >  1:141606/1‑140 (MQ=255)

                               tGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTg                                                                                                   <  1:807718/140‑1 (MQ=255)

                                 aaCTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCa                                                                                                 <  2:559592/140‑1 (MQ=255)

                                    ttCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGTACGt                                                                                              <  1:367482/140‑1 (MQ=255)

                                    ttCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGt                                                                                              <  1:852001/140‑1 (MQ=255)

                                         ggCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATg                                                                                         <  1:922915/140‑1 (MQ=255)

                                            gagaAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAg                                                                                      >  1:665028/1‑140 (MQ=255)

                                            gagaAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAg                                                                                      <  2:862052/140‑1 (MQ=255)

                                                aGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGtt                                                                                  <  1:1181073/140‑1 (MQ=255)

                                                     aaTCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCAtt                                                                             <  2:830301/140‑1 (MQ=255)

                                                           cTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGaa                                                                       <  2:438224/140‑1 (MQ=255)

                                                                    tAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGa                                                              >  2:199909/1‑140 (MQ=255)

                                                                       agCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTg                                                                            <  1:500081/123‑1 (MQ=255)

                                                                       agCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTg                                                                            >  2:500081/1‑123 (MQ=255)

                                                                        gCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCAc                                                          <  2:665028/140‑1 (MQ=255)

                                                                           gTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACggg                                                       <  1:27364/140‑1 (MQ=255)

                                                                            tCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACggac                                                      >  2:565195/1‑138 (MQ=255)

                                                                            tCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGc                                                      >  2:118929/1‑140 (MQ=255)

                                                                            tCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGc                                                      >  1:688261/1‑140 (MQ=255)

                                                                                  aCAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGtt                                                <  2:688261/140‑1 (MQ=255)

                                                                                    aaaGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTtca                                              <  1:199909/140‑1 (MQ=255)

                                                                                      aGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTtcatc                                            >  1:809001/1‑140 (MQ=255)

                                                                                          gTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCt                                        <  1:118929/140‑1 (MQ=255)

                                                                                           tAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGCGCAAGGTTCATCAGCtt                                       >  1:123229/1‑140 (MQ=255)

                                                                                            aGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCGCGACCACGGGCAAGGTTCATCAGCTTa                                      <  1:218638/140‑1 (MQ=255)

                                                                                                    ccccagacGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGga                              >  2:1178524/1‑140 (MQ=255)

                                                                                                          acGGTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCgg                        >  1:391697/1‑140 (MQ=255)

                                                                                                             gTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTc                     >  2:1204044/1‑140 (MQ=255)

                                                                                                             gTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTc                     >  1:83352/1‑140 (MQ=255)

                                                                                                             gTCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTc                     >  2:67241/1‑140 (MQ=255)

                                                                                                              tCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTc                     <  2:582627/139‑1 (MQ=255)

                                                                                                              tCTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTc                     >  1:582627/1‑139 (MQ=255)

                                                                                                               cTGAATGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCAc                   >  2:1036905/1‑140 (MQ=255)

                                                                                                                  aaTGTAACGCGGATGCGCTGGTTCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCAcgcg                <  1:1236953/140‑1 (MQ=255)

                                                                                                                  aaTGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCAcgcg                <  2:141606/140‑1 (MQ=255)

                                                                                                                    tGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGa              <  2:1214462/140‑1 (MQ=255)

                                                                                                                    tGTAACGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGa              <  1:67241/140‑1 (MQ=255)

                                                                                                                       aaCGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATgg           >  2:863696/1‑140 (MQ=255)

                                                                                                                        aCGCGGATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGc          <  1:863696/140‑1 (MQ=255)

                                                                                                                            ggATGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGAc      >  2:259609/1‑140 (MQ=255)

                                                                                                                              aTGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCa    >  2:732719/1‑140 (MQ=255)

                                                                                                                              aTGCGCTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCa    >  1:496739/1‑140 (MQ=255)

                                                                                                                                gcgcTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGt  <  2:809001/140‑1 (MQ=255)

                                                                                                                                gcgcTGGTTCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGt  <  2:155091/140‑1 (MQ=255)

AGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGT  >  NC_000913/3535805‑3536072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A