Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A31 F60 I1 R1	17	65.4	2242240	99.1%	2222059	138.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,436,474	G→A	A59V (GCC→GTC)	usg ←	putative semialdehyde dehydrogenase Usg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,436,474	0	G	A	100.0%	201.3 / NA	57	A59V (GCC→GTC)	usg	putative semialdehyde dehydrogenase Usg
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/26); total (31/26)

GAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGC  >  NC_000913/2436344‑2436609

gagaGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCa                                                                                                                                <  1:995226/140‑1 (MQ=255)

gagaGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCa                                                                                                                                <  1:644685/140‑1 (MQ=255)

                 tgctgTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTacca                                                                                                               >  1:752040/1‑140 (MQ=255)

                  gctgTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTaccac                                                                                                              <  1:350640/140‑1 (MQ=255)

                   ctgTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTg                                                                                                                           <  2:132541/126‑1 (MQ=255)

                   ctgTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTg                                                                                                                           >  1:132541/1‑126 (MQ=255)

                    tgTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTAccacca                                                                                                            <  1:1095564/140‑1 (MQ=255)

                    tgTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTAccacca                                                                                                            >  1:987448/1‑140 (MQ=255)

                    tgTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTAccacca                                                                                                            >  1:957666/1‑140 (MQ=255)

                       cGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAg                                                                                                         <  2:417358/140‑1 (MQ=255)

                          tCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAgcgc                                                                                                      <  2:496899/140‑1 (MQ=255)

                           cACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCa                                                                                                     >  2:1118075/1‑140 (MQ=255)

                           cACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCa                                                                                                     >  2:724274/1‑140 (MQ=255)

                             ccAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTAccacca                                                                                                            <  1:419449/131‑1 (MQ=255)

                             ccAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTAccacca                                                                                                            >  2:419449/1‑131 (MQ=255)

                              cAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAgtt                                                                                                  >  2:51801/1‑140 (MQ=255)

                                ggCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAgcgc                                                                                                      >  1:457119/1‑134 (MQ=255)

                                ggCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAgcgc                                                                                                      <  2:457119/134‑1 (MQ=255)

                                ggCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAgttgt                                                                                                >  2:334227/1‑140 (MQ=255)

                                     ccTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGcc                                                                                           >  2:626395/1‑140 (MQ=255)

                                         aGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTgcg                                                                                       <  1:833125/140‑1 (MQ=255)

                                          gTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTgcgc                                                                                      >  1:896004/1‑140 (MQ=255)

                                               tCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCg                                                                                 >  2:1089158/1‑140 (MQ=255)

                                               tCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCg                                                                                 >  2:1094746/1‑140 (MQ=255)

                                                             cAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGc                                                                   >  2:103114/1‑140 (MQ=255)

                                                               ggCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCAt                                                                 >  2:719082/1‑140 (MQ=255)

                                                               ggCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCAt                                                                 >  2:904727/1‑140 (MQ=255)

                                                                gtaGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGc                                                                         >  2:364086/3‑131 (MQ=255)

                                                                gtaGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGc                                                                         <  1:364086/129‑1 (MQ=255)

                                                                gCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATa                                                                >  2:1056058/1‑140 (MQ=255)

                                                                    cGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGc                                                                         >  1:1116523/1‑127 (MQ=255)

                                                                    cGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGc                                                                         <  2:1116523/127‑1 (MQ=255)

                                                                     ggTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGc                                                                         >  1:356049/1‑126 (MQ=255)

                                                                     ggTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGc                                                                         <  2:356049/126‑1 (MQ=255)

                                                                     ggTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAAttt                                                           >  2:214901/1‑140 (MQ=255)

                                                                           ttcttTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCaa                                                     <  2:929610/140‑1 (MQ=255)

                                                                                 gCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGa                                               >  2:181928/1‑140 (MQ=255)

                                                                                    tGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACt                                            >  2:92651/1‑140 (MQ=255)

                                                                                      cGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAAcc                                                   >  2:363097/1‑131 (MQ=255)

                                                                                      cGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAAcc                                                   <  1:363097/131‑1 (MQ=255)

                                                                                      cGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGa                                          >  2:156698/1‑140 (MQ=255)

                                                                                      cGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGa                                          >  1:173079/1‑140 (MQ=255)

                                                                                      cGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGa                                          >  1:520200/1‑140 (MQ=255)

                                                                                          aaaaaaTGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGtt                                      <  2:520200/140‑1 (MQ=255)

                                                                                                tGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTcagcca                                <  1:591542/140‑1 (MQ=255)

                                                                                                tGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTcagcca                                <  2:302463/140‑1 (MQ=255)

                                                                                                   cAGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCg                             >  2:699861/1‑140 (MQ=255)

                                                                                                    aGCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGt                            <  2:994763/140‑1 (MQ=255)

                                                                                                     gCTGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGtt                           <  1:904727/140‑1 (MQ=255)

                                                                                                       tGCGCCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTc                         <  1:699861/140‑1 (MQ=255)

                                                                                                          gcCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTcagcca                                <  1:187569/130‑1 (MQ=255)

                                                                                                          gcCTGCGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTcagcca                                >  2:187569/1‑130 (MQ=255)

                                                                                                               cGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTcagcc                                 >  1:218340/1‑124 (MQ=255)

                                                                                                               cGTCCAGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTcagcc                                 <  2:218340/124‑1 (MQ=255)

                                                                                                                    aGTCGAATTCAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCg            <  1:724274/140‑1 (MQ=255)

                                                                                                                             cAGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAgcg   <  1:156698/140‑1 (MQ=255)

                                                                                                                              aGCGACATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAgcgc  <  2:752040/140‑1 (MQ=255)

GAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGC  >  NC_000913/2436344‑2436609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A