Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A35 F60 I1 R1	20	36.7	1244762	99.1%	1233559	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,284	A→G	noncoding (12/143 nt)	ryfD ←	small regulatory RNA RyfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,284	0	A	G	100.0%	190.3 / NA	49	noncoding (12/143 nt)	ryfD	small regulatory RNA RyfD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/24); total (25/24)

TTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCG  >  NC_000913/2734147‑2734402

tttATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTc                                                                                                                      >  2:281478/1‑140 (MQ=255)

     taGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCtt                                                                                                                 <  1:281478/140‑1 (MQ=255)

      aGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTg                                                                                                                <  1:330498/140‑1 (MQ=255)

       gTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTg                                                                                                                <  1:285073/139‑1 (MQ=255)

       gTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTg                                                                                                                >  2:285073/1‑139 (MQ=255)

            aCGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCtt                                                                                                          <  2:199873/140‑1 (MQ=255)

                 ccAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGt                                                                                                     >  2:375639/1‑140 (MQ=255)

                      cGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTatat                                                                                                <  2:381327/140‑1 (MQ=255)

                      cGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTatat                                                                                                >  1:381327/1‑140 (MQ=255)

                         aTAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAgg                                                                                                      >  1:609951/1‑131 (MQ=255)

                         aTAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAgg                                                                                                      <  2:609951/131‑1 (MQ=255)

                          tAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAg                                                                                             <  1:100984/139‑1 (MQ=255)

                          tAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAg                                                                                             >  2:100984/1‑139 (MQ=255)

                                ctccCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATg                                                                                      >  2:366064/1‑140 (MQ=255)

                                   ccATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGaaa                                                                                   >  1:513479/1‑140 (MQ=255)

                                    cATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAAc                                                                                  <  2:298018/140‑1 (MQ=255)

                                          ggATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCa                                                                            >  2:4485/1‑140 (MQ=255)

                                           gATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCAt                                                                           <  2:240766/140‑1 (MQ=255)

                                             tCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTC‑TTAGGTTATATCAGCCAAATGAAACTTGCCATACg                                                                        <  2:494778/140‑1 (MQ=255)

                                              cAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACg                                                                        >  2:187395/1‑140 (MQ=255)

                                                 aGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGAcc                                                                     >  1:531998/1‑140 (MQ=255)

                                                  gTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCg                                                                    <  2:531998/140‑1 (MQ=255)

                                                            gagaTTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGt                                                          >  2:71366/1‑140 (MQ=255)

                                                                 tAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGcga                                                     >  1:277785/1‑140 (MQ=255)

                                                                  aaaTGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGcgac                                                    <  2:455862/140‑1 (MQ=255)

                                                                    aTGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGcgacga                                                  <  1:71366/140‑1 (MQ=255)

                                                                         gTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAaga                                             >  1:286108/1‑140 (MQ=255)

                                                                         gTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAACCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAaga                                             >  2:527714/1‑140 (MQ=255)

                                                                            aTCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTc                                                         >  2:19540/1‑125 (MQ=255)

                                                                            aTCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTc                                                         <  1:19540/125‑1 (MQ=255)

                                                                            aTCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGagaa                                          <  2:286108/140‑1 (MQ=255)

                                                                              cccTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAG‑AAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAgaagaa                                       >  1:588545/1‑140 (MQ=255)

                                                                                cTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGaaa                                      <  2:277785/140‑1 (MQ=255)

                                                                                     ttattCAAGGTTATTGACCAGATTAATGTGAAAAG‑AAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCa                                <  2:588545/140‑1 (MQ=255)

                                                                                        ttCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCAtt                              >  2:525942/1‑140 (MQ=255)

                                                                                        ttCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCAtt                              >  2:96658/1‑140 (MQ=255)

                                                                                           aaGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTc                           <  1:366064/140‑1 (MQ=255)

                                                                                             ggTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                         <  1:187395/140‑1 (MQ=255)

                                                                                              gTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGt                        <  2:513479/140‑1 (MQ=255)

                                                                                              gTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGt                        <  1:4485/140‑1 (MQ=255)

                                                                                                 aTTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                         >  1:57991/1‑136 (MQ=255)

                                                                                                 aTTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                         <  2:57991/136‑1 (MQ=255)

                                                                                                  ttGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTatat                    >  2:574708/1‑140 (MQ=255)

                                                                                                       cAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                         <  2:141609/130‑1 (MQ=255)

                                                                                                       cAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                         >  1:141609/1‑130 (MQ=255)

                                                                                                       cAGATTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACa               <  1:525942/140‑1 (MQ=255)

                                                                                                          aTTAATGTGAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACg            >  1:196409/1‑140 (MQ=255)

                                                                                                               tgtgAAAAGAAAATCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTcgc       >  1:473836/1‑140 (MQ=255)

                                                                                                                    aaagaaaaTCACGCGTACCGGGTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGccgccg  >  2:513546/1‑140 (MQ=255)

TTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCG  >  NC_000913/2734147‑2734402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A