Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A36 F60 I1 R1 17 116.8 3946860 99.2% 3915285 138.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,407,734 G→A V58V (GTG→GTA)  alaA → glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,407,7340GA98.7% 536.8 / ‑4.0 153V58V (GTG→GTA) alaAglutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (70/81);  total (71/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

TCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTG  >  NC_000913/2407598‑2407870
                                                                                                                                        |                                                                                                                                        
tCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATa                                                                                                                                       <  2:1914706/140‑1 (MQ=255)
   gTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGc                                                                                                                                    <  1:449479/140‑1 (MQ=255)
         aCATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGc                                                                                                                                    <  2:1430876/134‑1 (MQ=255)
          cATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGc                                                                                                                             >  2:279837/1‑140 (MQ=255)
          cATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGc                                                                                                                             >  1:1405995/1‑140 (MQ=255)
             ccGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTa                                                                                                                          <  2:1283299/140‑1 (MQ=255)
             ccGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTa                                                                                                                          <  2:569017/140‑1 (MQ=255)
             ccGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTa                                                                                                                          <  2:1872577/140‑1 (MQ=255)
              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGc                                                                                                                                    <  1:315716/129‑1 (MQ=255)
              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTAc                                                                                                                         >  2:1024023/1‑140 (MQ=255)
              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTAc                                                                                                                         <  1:48871/140‑1 (MQ=255)
                tGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCg                                                                                                                       <  1:1068467/140‑1 (MQ=255)
                     cGGTGCTGAAAGAAGCAGAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCaa                                                                                                                  >  1:29448/1‑140 (MQ=255)
                     cGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCaa                                                                                                                  <  2:1557619/140‑1 (MQ=255)
                       gTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCt                                                                                                                     <  2:430758/135‑1 (MQ=255)
                       gTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGACAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAgg                                                                                                                >  2:745902/1‑140 (MQ=255)
                             aaaGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTa                                                                                                                          <  2:704244/124‑1 (MQ=255)
                             aaaGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTa                                                                                                                          >  1:704244/1‑124 (MQ=255)
                             aaaGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTc                                                                                                                    >  2:762691/1‑130 (MQ=255)
                             aaaGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTg                                                                                                          >  2:345041/1‑140 (MQ=255)
                             aaaGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTg                                                                                                          >  1:1814683/1‑140 (MQ=255)
                                gaagCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGa                                                                                                       <  1:1250693/140‑1 (MQ=255)
                                  agCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTc                                                                                                                    >  2:1742048/1‑125 (MQ=255)
                                  agCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGAtt                                                                                                     >  1:1864464/1‑140 (MQ=255)
                                  agCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGAtt                                                                                                     >  1:1704171/1‑140 (MQ=255)
                                  agCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATAAGCAACCTGCCTACCGCTCAAGGGTATTGCGAtt                                                                                                     >  1:980807/1‑140 (MQ=255)
                                    cAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCTAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTcc                                                                                                   >  2:166971/1‑140 (MQ=255)
                                     aaaaCGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                                                                                                  <  1:1415338/140‑1 (MQ=255)
                                       aaCGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCaaa                                                                                                <  2:1280992/140‑1 (MQ=255)
                                       aaCGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCaaa                                                                                                <  1:345041/140‑1 (MQ=255)
                                       aaCGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCaaa                                                                                                <  1:1312953/140‑1 (MQ=255)
                                           ccTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTc                                                                                            >  2:1438657/1‑140 (MQ=255)
                                                    agaagGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCcg                                                                                   <  1:1024023/140‑1 (MQ=255)
                                                    agaagGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCcg                                                                                   <  2:768559/140‑1 (MQ=255)
                                                     gaagGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCcgc                                                                                  >  1:1089648/1‑140 (MQ=255)
                                                     gaagGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                                                                                                  >  1:1908052/1‑124 (MQ=255)
                                                      aagGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCaaa                                                                                                <  1:446833/125‑1 (MQ=255)
                                                            aCAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTaaa                                                                           >  2:1594273/1‑140 (MQ=255)
                                                             cAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCcg                                                                                   >  2:1833795/1‑131 (MQ=255)
                                                             cAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAg                                                                          <  1:1723125/140‑1 (MQ=255)
                                                                ggTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCca                                                                       >  2:860911/1‑140 (MQ=255)
                                                                  tACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGTGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCcatc                                                                     >  1:1564447/1‑140 (MQ=255)
                                                                  tACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCcatc                                                                     >  1:11644/1‑140 (MQ=255)
                                                                  tACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCcatc                                                                     >  2:244029/1‑140 (MQ=255)
                                                                  tACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCca                                                                       >  2:876110/1‑138 (MQ=255)
                                                                  tACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCca                                                                       <  1:876110/138‑1 (MQ=255)
                                                                   actgaaactgaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCcatca                                                                    <  2:429077/140‑1 (MQ=255)
                                                                   actgaaactgaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCcatca                                                                    >  1:429077/1‑140 (MQ=255)
                                                                    ctgaaactgaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCcatcat                                                                   >  1:754851/1‑140 (MQ=255)
                                                                    ctgaaactgaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCcatcat                                                                   >  2:160858/1‑140 (MQ=255)
                                                                        aactgaaCATCGGCAACCTAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATgcag                                                               <  1:166971/140‑1 (MQ=255)
                                                                        aactgaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATgcag                                                               <  1:143699/140‑1 (MQ=255)
                                                                        aactgaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATgcag                                                               <  2:1923375/140‑1 (MQ=255)
                                                                        aactgaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATgcag                                                               <  2:903684/140‑1 (MQ=255)
                                                                        aactgaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATgcag                                                               <  2:11644/140‑1 (MQ=255)
                                                                           tgaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCAc                                                            >  2:1434587/1‑140 (MQ=255)
                                                                            gaaCATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACt                                                           >  1:223474/1‑140 (MQ=255)
                                                                              aCATCGACAACCCAGCCCCGTTCGGATTTGACGCGCCAGATGAAATCCTCGATGACGTAATACGCAACCTGCCTACCGCTCAGGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTAc                                                         >  2:648954/1‑140 (MQ=255)
                                                                                  cGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATgca                                                                <  1:1093244/129‑1 (MQ=255)
                                                                                  cGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAgg                                                     >  1:459216/1‑140 (MQ=255)
                                                                                  cGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAgg                                                     >  1:1608369/1‑140 (MQ=255)
                                                                                    gCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCt                                                   <  2:5109/140‑1 (MQ=255)
                                                                                    gCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCt                                                   <  2:1564262/140‑1 (MQ=255)
                                                                                       aCCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCt                                                   >  1:839611/1‑137 (MQ=255)
                                                                                          cAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGc                                             <  1:244029/140‑1 (MQ=255)
                                                                                           aGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCa                                            <  2:1089648/140‑1 (MQ=255)
                                                                                           aGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCa                                            <  2:1566594/140‑1 (MQ=255)
                                                                                             ccccGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTATCAGGCTCGTGGCATg                                          <  1:1040009/140‑1 (MQ=255)
                                                                                             ccccGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATg                                          >  1:1748225/1‑140 (MQ=255)
                                                                                               ccGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCg                                        <  1:1594273/140‑1 (MQ=255)
                                                                                               ccGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCg                                        <  2:29448/140‑1 (MQ=255)
                                                                                               ccGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCg                                        <  1:672807/140‑1 (MQ=255)
                                                                                                  ttCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCa                                                       <  2:451138/122‑1 (MQ=255)
                                                                                                  ttCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGa                                     >  1:781371/1‑140 (MQ=255)
                                                                                                  ttCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGa                                     >  2:1625360/1‑140 (MQ=255)
                                                                                                  ttCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGa                                     >  2:1611594/1‑140 (MQ=255)
                                                                                                  ttCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGa                                     >  2:1057774/1‑140 (MQ=255)
                                                                                                  ttCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGa                                     >  1:41058/1‑140 (MQ=255)
                                                                                                  ttCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACATGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGa                                     >  1:1495568/1‑140 (MQ=255)
                                                                                                   tCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGAt                                    >  2:646194/1‑140 (MQ=255)
                                                                                                       ttttGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTa                                >  1:1451871/1‑140 (MQ=255)
                                                                                                       ttttGACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTa                                <  1:1625360/140‑1 (MQ=255)
                                                                                                           gACGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGt                            <  2:1153496/140‑1 (MQ=255)
                                                                                                            aCGCGCCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTg                           >  2:338965/1‑140 (MQ=255)
                                                                                                              gcgcCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGa                         <  2:459216/140‑1 (MQ=255)
                                                                                                              gcgcCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGa                         <  2:41058/140‑1 (MQ=255)
                                                                                                               cgcCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGaa                        <  2:223474/140‑1 (MQ=255)
                                                                                                               cgcCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGaa                        <  2:1814683/140‑1 (MQ=255)
                                                                                                                gcCAGATGAAATCCTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATACAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAg                       <  1:273763/140‑1 (MQ=255)
                                                                                                                             cTCGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCgg          <  1:277481/140‑1 (MQ=255)
                                                                                                                               cGTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCa        >  1:105326/1‑140 (MQ=255)
                                                                                                                                gTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCaa       <  1:709199/140‑1 (MQ=255)
                                                                                                                                gTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCaa       >  2:709199/1‑140 (MQ=255)
                                                                                                                                gTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCaa       <  2:138018/140‑1 (MQ=255)
                                                                                                                                gTTGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCaa       >  1:1632050/1‑140 (MQ=255)
                                                                                                                                  tGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATg     <  1:1434587/140‑1 (MQ=255)
                                                                                                                                  tGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATg     <  2:1451871/140‑1 (MQ=255)
                                                                                                                                  tGACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATg     >  1:1012134/1‑140 (MQ=255)
                                                                                                                                   gACGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATgg    >  1:1071351/1‑140 (MQ=255)
                                                                                                                                     cGTAATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGgtg  <  1:646194/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTG  >  NC_000913/2407598‑2407870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A