Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	117	80.8	2798880	97.5%	2728908	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,701,540	T→C	T64A (ACG→GCG)	pdxJ ←	pyridoxine 5'‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,701,540	0	T	C	100.0%	161.5 / NA	45	T64A (ACG→GCG)	pdxJ	pyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/17); total (28/17)

GACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCG  >  NC_000913/2701403‑2701676

gACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCt                                                                                                                                        >  2:1054434/1‑140 (MQ=255)

    tCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACg                                                                                                                                    >  2:628847/1‑140 (MQ=255)

    tCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGC‑AAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGc                                                                                                                                   <  1:759616/140‑1 (MQ=255)

         gccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGa                                                                                                                               >  2:466500/1‑140 (MQ=255)

             ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATgcg                                                                                                                           >  1:150286/1‑140 (MQ=255)

              cTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAgg                                                                                                                                <  1:597985/134‑1 (MQ=255)

              cTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAgg                                                                                                                                >  2:597985/1‑134 (MQ=255)

               ttCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCa                                                                                                                         >  1:470409/1‑140 (MQ=255)

                      gttACTTCCTGACGCTTTTCCGGTGCCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTcgcg                                                                                                                  <  2:362751/140‑1 (MQ=255)

                      gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTcgcg                                                                                                                  <  1:354608/140‑1 (MQ=255)

                      gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTcgcg                                                                                                                  <  1:628847/140‑1 (MQ=255)

                                gACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtg                                                                                                        <  2:1357197/140‑1 (MQ=255)

                                      tttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACgg                                                                                                  <  1:293538/140‑1 (MQ=255)

                                       ttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGc                                                                                                 >  1:20460/1‑140 (MQ=255)

                                               ccAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCg                                                                                                >  1:396787/1‑133 (MQ=255)

                                               ccAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCg                                                                                                <  2:396787/133‑1 (MQ=255)

                                                               tgGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg                                                                                 >  1:944833/1‑132 (MQ=255)

                                                               tgGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg                                                                                 <  2:944833/132‑1 (MQ=255)

                                                                             cGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTccc                                                           >  1:967270/1‑140 (MQ=255)

                                                                             cGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTccc                                                           <  1:1054434/140‑1 (MQ=255)

                                                                                  aTCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg                                                      >  1:592549/1‑140 (MQ=255)

                                                                                  aTCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg                                                      >  1:1030324/1‑140 (MQ=255)

                                                                                  aTCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg                                                      >  2:408725/1‑140 (MQ=255)

                                                                                  aTCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGCGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg                                                      <  2:20460/140‑1 (MQ=255)

                                                                                    cGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCt                                                    >  1:497824/1‑140 (MQ=255)

                                                                                    cGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCt                                                    <  2:150286/140‑1 (MQ=255)

                                                                                             ctctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATaa                                           >  1:629109/1‑140 (MQ=255)

                                                                                                  cGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAAcgcg                                      <  1:466500/140‑1 (MQ=255)

                                                                                                    gTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGc                                    <  1:379740/140‑1 (MQ=255)

                                                                                                       aCCGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTg                                 >  1:517254/1‑140 (MQ=255)

                                                                                                        ccGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGc                                    <  2:526506/136‑1 (MQ=255)

                                                                                                        ccGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGc                                    >  1:526506/1‑136 (MQ=255)

                                                                                                        ccGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGc                                >  1:428556/1‑140 (MQ=255)

                                                                                                        ccGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGc                                >  2:1159623/1‑140 (MQ=255)

                                                                                                        ccGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGc                                >  2:804210/1‑140 (MQ=255)

                                                                                                         cGCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCa                               >  1:712505/1‑140 (MQ=255)

                                                                                                         cGCCATCTCAAGATTCATGCGGGTATCCCGCGCCTAACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGGGATCTTTACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCa                               <  2:967270/140‑1 (MQ=255)

                                                                                                          gCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCAc                              >  1:629318/1‑140 (MQ=255)

                                                                                                          gCCATCTCCAGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCAc                              >  2:826720/1‑140 (MQ=255)

                                                                                                                   aGATTCATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCgg                     <  1:941297/140‑1 (MQ=255)

                                                                                                                        cATGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAg                >  2:576255/1‑140 (MQ=255)

                                                                                                                         aTGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGc               >  1:427794/1‑140 (MQ=255)

                                                                                                                          tGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCg              >  2:237532/1‑140 (MQ=255)

                                                                                                                          tGCGGGTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCg              >  2:103896/1‑140 (MQ=255)

                                                                                                                              ggTATCCAGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTAc          <  1:237532/140‑1 (MQ=255)

                                                                                                                                     aGCGCCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcgc   >  1:15923/1‑140 (MQ=255)

                                                                                                                                      gcgcCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACTCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcgcg  <  1:318427/140‑1 (MQ=255)

GACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCG  >  NC_000913/2701403‑2701676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A