Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F30 I1 R1	25	134.5	4568722	99.0%	4523034	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,573,868	A→T	V485V (GTA→GTT)	yjiT →	putative uncharacterized protein YjiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,573,868	0	A	T	99.4%	569.8 / ‑4.3	159	V485V (GTA→GTT)	yjiT	putative uncharacterized protein YjiT
Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (70/88); total (70/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 159 total aligned reads displayed.

GTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAA  >  NC_000913/4573732‑4573998

gTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCa                                                                                                                                 >  2:1964815/1‑140 (MQ=255)

 tCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCaa                                                                                                                                >  2:607756/1‑140 (MQ=255)

   aGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCaaaa                                                                                                                              <  2:2050156/140‑1 (MQ=255)

   aGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCaaaa                                                                                                                              >  2:638421/1‑140 (MQ=255)

     gACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGt                                                                                                                            <  2:835562/140‑1 (MQ=255)

     gACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGt                                                                                                                            <  1:1431194/140‑1 (MQ=255)

      aCGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGt                                                                                                                            >  2:1389767/1‑139 (MQ=255)

      aCGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGt                                                                                                                            <  1:1389767/139‑1 (MQ=255)

        gttacagttacaGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGt                                                                                                                            >  1:599715/1‑137 (MQ=255)

        gttacagttacaGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGt                                                                                                                            <  2:599715/137‑1 (MQ=255)

        gttacagttacaGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCAt                                                                                                                         <  1:521496/140‑1 (MQ=255)

        gttacagttacaGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCAt                                                                                                                         <  2:1002098/140‑1 (MQ=255)

        gttacagttacaGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCAt                                                                                                                         <  2:1877509/140‑1 (MQ=255)

          tacagttacagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCAttt                                                                                                                       >  1:1215001/1‑140 (MQ=255)

          tacagttacagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCAttt                                                                                                                       >  2:1158889/1‑140 (MQ=255)

             agttacagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTa                                                                                                                    <  1:1088766/140‑1 (MQ=255)

             agttacagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTa                                                                                                                    <  1:607756/140‑1 (MQ=255)

             agttacagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTa                                                                                                                    <  1:626639/140‑1 (MQ=255)

             agttacagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTa                                                                                                                    <  2:1347944/140‑1 (MQ=255)

                 acagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACt                                                                                                                <  2:1532775/140‑1 (MQ=255)

                 acagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACt                                                                                                                <  1:1964815/140‑1 (MQ=255)

                  cagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTc                                                                                                                           >  1:1139553/1‑128 (MQ=255)

                   agCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTAc                                                                                                              >  2:256400/1‑140 (MQ=255)

                    gCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTAcc                                                                                                             >  2:1462524/1‑140 (MQ=255)

                         tGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACt                                                                                                                >  1:2109374/1‑132 (MQ=255)

                         tGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTAcc                                                                                                             >  1:2115790/1‑135 (MQ=255)

                         tGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGata                                                                                                        >  1:1476922/1‑140 (MQ=255)

                               ttATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCaa                                                                                                   >  2:2184646/1‑139 (MQ=255)

                               ttATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCaa                                                                                                   <  1:2184646/139‑1 (MQ=255)

                                  tCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCa                                                                                               >  2:604832/1‑140 (MQ=255)

                                   cGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCAt                                                                                              <  1:2231438/140‑1 (MQ=255)

                                     gATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTg                                                                                            <  1:1506150/140‑1 (MQ=255)

                                      aTTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGa                                                                                           >  2:1957790/1‑140 (MQ=255)

                                       ttCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGAt                                                                                          >  1:1161425/1‑140 (MQ=255)

                                         cGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGt                                                                                        >  1:1324249/1‑140 (MQ=255)

                                           cACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTaa                                                                                      >  1:765935/1‑140 (MQ=255)

                                           cACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTaa                                                                                      <  2:1324249/140‑1 (MQ=255)

                                            aCCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAAc                                                                                     >  2:1283023/1‑140 (MQ=255)

                                              cGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAAcgc                                                                                   <  1:604832/140‑1 (MQ=255)

                                                  agagAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTa                                                                               <  2:1694404/140‑1 (MQ=255)

                                                      aaCAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTa                                                                                       <  2:1859899/128‑1 (MQ=255)

                                                      aaCAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTa                                                                                       >  1:1859899/1‑128 (MQ=255)

                                                      aaCAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACg                                                                           >  1:1364332/1‑140 (MQ=255)

                                                      aaCAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACg                                                                           >  1:732172/1‑140 (MQ=255)

                                                          aaTCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCt                                                                       <  1:688861/140‑1 (MQ=255)

                                                             cAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGAc                                                                    >  2:1373921/1‑140 (MQ=255)

                                                              aGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGa                                                                     >  1:1774136/1‑138 (MQ=255)

                                                               gCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACaa                                                                  >  1:1429149/1‑140 (MQ=255)

                                                                cATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGa                                                                     >  2:1655820/1‑136 (MQ=255)

                                                                cATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGa                                                                     <  1:508660/136‑1 (MQ=255)

                                                                cATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGa                                                                     >  2:508660/1‑136 (MQ=255)

                                                                cATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGa                                                                     <  1:1655820/136‑1 (MQ=255)

                                                                cATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACaaa                                                                 >  2:1982732/1‑140 (MQ=255)

                                                                cATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACaaa                                                                 >  2:1769258/1‑140 (MQ=255)

                                                                cATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACaaa                                                                 >  1:796420/1‑140 (MQ=255)

                                                                 aTCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACaaaa                                                                <  1:256400/140‑1 (MQ=255)

                                                                   cGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACa                                                              <  2:2140278/140‑1 (MQ=255)

                                                                   cGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACa                                                              <  1:200026/140‑1 (MQ=255)

                                                                   cGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACa                                                              >  1:1303792/1‑140 (MQ=255)

                                                                   cGGAAGG‑TTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGt                                                                          >  2:1139274/1‑127 (MQ=255)

                                                                   cGGAAGG‑TTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATGGATGTAACGCGTTACACGt                                                                          <  1:1139274/127‑1 (MQ=255)

                                                                     gAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGc                                                            >  2:890902/1‑140 (MQ=255)

                                                                          tttGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTAc                                                       >  2:1815114/1‑140 (MQ=255)

                                                                          tttGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTAc                                                       >  1:1288811/1‑140 (MQ=255)

                                                                            tGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTAcag                                                     >  2:853437/1‑140 (MQ=255)

                                                                             gCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTAcagc                                                    <  1:1815114/140‑1 (MQ=255)

                                                                             gCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTAcagc                                                    >  2:482222/1‑140 (MQ=255)

                                                                             gCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTAcagc                                                    <  1:989395/140‑1 (MQ=255)

                                                                               tctAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACATGTGCTGACAAAACAGCATTAcagcca                                                  <  1:1643263/140‑1 (MQ=255)

                                                                                    aTGGAAAGCGTGCAAGCTGGGTTTGTCGTCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAgg                                             <  1:1722745/140‑1 (MQ=255)

                                                                                    aTGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTAcagcca                                                  <  1:868616/135‑1 (MQ=255)

                                                                                    aTGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAgg                                             <  2:1476922/140‑1 (MQ=255)

                                                                                        aaaGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGt                                         >  1:1899980/1‑140 (MQ=255)

                                                                                             gTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAgg                                             >  2:454951/1‑131 (MQ=255)

                                                                                             gTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAgg                                             <  1:454951/131‑1 (MQ=255)

                                                                                             gTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGc                                    >  1:2238594/1‑140 (MQ=255)

                                                                                              tGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCg                                   >  2:733258/1‑140 (MQ=255)

                                                                                               gCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTa                                        >  1:1329131/1‑134 (MQ=255)

                                                                                               gCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTa                                        <  2:1329131/134‑1 (MQ=255)

                                                                                                 aaGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGGCAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTg                                >  1:341481/1‑140 (MQ=255)

                                                                                                     tGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATc                            <  2:1161425/140‑1 (MQ=255)

                                                                                                     tGGGTTTGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATc                            <  1:2017605/140‑1 (MQ=255)

                                                                                                         tttGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATg                        <  2:143784/140‑1 (MQ=255)

                                                                                                          ttGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGt                       <  2:1613545/140‑1 (MQ=255)

                                                                                                           tGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTc                      <  2:211231/140‑1 (MQ=255)

                                                                                                           tGTCATCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTc                      <  2:130379/140‑1 (MQ=255)

                                                                                                                tCCAGATGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTc                 <  1:890902/140‑1 (MQ=255)

                                                                                                                     aTGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGa            >  1:1723157/1‑140 (MQ=255)

                                                                                                                     aTGAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGa            <  1:111455/140‑1 (MQ=255)

                                                                                                                       gAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACt          <  1:1909909/140‑1 (MQ=255)

                                                                                                                       gAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACt          >  2:203503/1‑140 (MQ=255)

                                                                                                                       gAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACt          <  1:902041/140‑1 (MQ=255)

                                                                                                                       gAAACATTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACt          <  1:1373921/140‑1 (MQ=255)

                                                                                                                            aTTTATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAAcc     <  1:733258/140‑1 (MQ=255)

                                                                                                                              ttATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTa   <  2:326944/140‑1 (MQ=255)

                                                                                                                              ttATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTa   >  1:1314071/1‑140 (MQ=255)

                                                                                                                               tATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAg               >  1:152808/1‑127 (MQ=255)

                                                                                                                               tATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAg               <  2:152808/127‑1 (MQ=255)

                                                                                                                               tATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTaa  >  2:1929825/1‑140 (MQ=255)

                                                                                                                               tATTGGTGTTCCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTaa  >  2:1733269/1‑140 (MQ=255)

GTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAA  >  NC_000913/4573732‑4573998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A